Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP14_F_A11.2
(1326 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 404 e-113
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 400 e-112
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 206 5e-54
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 167 2e-42
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 32 0.20
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 404 bits (994), Expect = e-113
Identities = 180/254 (70%), Positives = 214/254 (84%)
Frame = +1
Query: 142 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 321
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 322 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 501
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 502 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 681
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 682 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 861
VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITA
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240
Query: 862 SLRFDGALNVDLTE 903
SLRF+G+LNVDL E
Sbjct: 241 SLRFEGSLNVDLAE 254
Score = 102 bits (245), Expect = 1e-22
Identities = 46/71 (64%), Positives = 51/71 (71%)
Frame = +3
Query: 906 QTNLVPYPRIHFPLVTYAPVISXXKAYXEQXSVAXIXNACFEPANXMVKXXPGHGKXMAX 1085
QTNLVPYPRIHFPLVTYAP++S KA+ E SV I N CFEP N MVK P G+ MA
Sbjct: 256 QTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMAT 315
Query: 1086 CMLYRGXXXPR 1118
C+LYRG PR
Sbjct: 316 CLLYRGDVIPR 326
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 400 bits (986), Expect = e-112
Identities = 184/259 (71%), Positives = 216/259 (83%), Gaps = 5/259 (1%)
Frame = +1
Query: 142 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 306
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 307 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 486
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 487 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 666
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 667 QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIV 846
QVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+V
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239
Query: 847 SSITASLRFDGALNVDLTE 903
SSITASLRF G+LNVDL E
Sbjct: 240 SSITASLRFAGSLNVDLNE 258
Score = 101 bits (242), Expect = 2e-22
Identities = 45/71 (63%), Positives = 51/71 (71%)
Frame = +3
Query: 906 QTNLVPYPRIHFPLVTYAPVISXXKAYXEQXSVAXIXNACFEPANXMVKXXPGHGKXMAX 1085
QTNLVPYPRIHFPLVTY+P++S KA+ E SV I N CFEP N MVK P G+ MA
Sbjct: 260 QTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMAT 319
Query: 1086 CMLYRGXXXPR 1118
C+LYRG PR
Sbjct: 320 CLLYRGDVIPR 330
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 206 bits (503), Expect = 5e-54
Identities = 100/252 (39%), Positives = 144/252 (57%)
Frame = +1
Query: 142 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 321
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 322 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 501
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 502 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 681
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 682 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 861
VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTT 238
Query: 862 SLRFDGALNVDL 897
S RF G LN DL
Sbjct: 239 SFRFPGELNSDL 250
Score = 53.2 bits (122), Expect = 8e-08
Identities = 22/68 (32%), Positives = 38/68 (55%)
Frame = +3
Query: 900 RVQTNLVPYPRIHFPLVTYAPVISXXKAYXEQXSVAXIXNACFEPANXMVKXXPGHGKXM 1079
++ N+VP+PR+HF +V +AP+ + + + SV + F+ N MV P HG+ +
Sbjct: 252 KLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYL 311
Query: 1080 AXCMLYRG 1103
L+RG
Sbjct: 312 TVAALFRG 319
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 167 bits (407), Expect = 2e-42
Identities = 90/254 (35%), Positives = 143/254 (56%), Gaps = 3/254 (1%)
Frame = +1
Query: 145 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 324
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 325 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVL 498
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 499 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 675
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 676 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSI 855
VV+PYNS+L ++D ++DN A+ I L + PT+ N+L+ ++S+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239
Query: 856 TASLRFDGALNVDL 897
T +LR+ G +N DL
Sbjct: 240 TTTLRYPGYMNNDL 253
Score = 27.9 bits (59), Expect = 3.3
Identities = 17/54 (31%), Positives = 24/54 (44%), Gaps = 4/54 (7%)
Frame = +3
Query: 912 NLVPYPRIHFPLVTYAPV----ISXXKAYXEQXSVAXIXNACFEPANXMVKXXP 1061
+L+P PR HF L +Y P + KA + +V + P N MV P
Sbjct: 259 SLIPSPRCHFLLTSYTPFTNQQVEEAKA-IRKTTVLDVMRRLLLPKNQMVSVNP 311
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 31.9 bits (69), Expect = 0.20
Identities = 16/43 (37%), Positives = 26/43 (60%)
Frame = -1
Query: 891 HIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 763
H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 176 HRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,197,742
Number of Sequences: 5004
Number of extensions: 83895
Number of successful extensions: 253
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 237
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 248
length of database: 2,362,478
effective HSP length: 75
effective length of database: 1,987,178
effective search space used: 727307148
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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