Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP12_F_J06
(960 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 314 2e-86
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 309 5e-85
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 161 1e-40
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 132 9e-32
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S... 31 0.32
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 9.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 9.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 314 bits (770), Expect = 2e-86
Identities = 137/200 (68%), Positives = 165/200 (82%)
Frame = +2
Query: 167 HVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVF 346
HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+VPR+++
Sbjct: 8 HVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIY 67
Query: 347 VDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLAD 526
VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD
Sbjct: 68 VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127
Query: 527 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNS 706
C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS
Sbjct: 128 NCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNS 187
Query: 707 ILTTHTTLEHSDCAFMVDNE 766
+LTTH TL+ +DC FMVDNE
Sbjct: 188 VLTTHATLDLADCTFMVDNE 207
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 309 bits (758), Expect = 5e-85
Identities = 138/205 (67%), Positives = 168/205 (81%), Gaps = 5/205 (2%)
Frame = +2
Query: 167 HVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHV 331
HVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSETG GK V
Sbjct: 8 HVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFV 66
Query: 332 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 511
PR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RI
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 512 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVV 691
R++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST+VV
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVV 186
Query: 692 EPYNSILTTHTTLEHSDCAFMVDNE 766
EPYNS+LTTH TL++SDC FMVDNE
Sbjct: 187 EPYNSVLTTHATLDNSDCTFMVDNE 211
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 161 bits (391), Expect = 1e-40
Identities = 79/198 (39%), Positives = 112/198 (56%)
Frame = +2
Query: 173 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 352
GQ G Q+G A W EHG+ G T + N +F+E GK+VPRAV VD
Sbjct: 10 GQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVD 67
Query: 353 LEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQC 532
LEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 533 TGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSIL 712
LQGF + H LL+ ++ +Y + F++ PAP+ S VVEPYN+ L
Sbjct: 128 DALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATL 187
Query: 713 TTHTTLEHSDCAFMVDNE 766
+ H +E+SD F +DNE
Sbjct: 188 SMHQLVENSDETFCIDNE 205
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 132 bits (318), Expect = 9e-32
Identities = 72/200 (36%), Positives = 112/200 (56%), Gaps = 3/200 (1%)
Frame = +2
Query: 173 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 352
GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +++PRA+ +D
Sbjct: 11 GQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILID 68
Query: 353 LEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLAD 526
LEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D I + AD
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 527 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYN 703
L+GF + H L+ERL+ Y KK ++++P Q VS VV+PYN
Sbjct: 128 GSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYN 187
Query: 704 SILTTHTTLEHSDCAFMVDN 763
S+L ++D ++DN
Sbjct: 188 SLLALKRLTLNADSVVVLDN 207
>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 30.7 bits (66), Expect = 0.32
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +2
Query: 626 YGKKSKLEFAIYPAPQVSTAVVE-PYNSILTTHTTLEHSDCAFMVDNEXSM 775
Y + K+E YP ++ V + PYN +L ++LE ++DN S+
Sbjct: 110 YNSRWKIEIPSYPVREIPYVVADTPYNKLLCPLSSLEWVSKLILMDNANSI 160
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 9.1
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 225 STASSLMARCPQTRPSGVETILSTLSSARPELAST 329
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 9.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 244 WPDAHRQDHRGWRRFFQHFLQR 309
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,230,573
Number of Sequences: 5004
Number of extensions: 65307
Number of successful extensions: 175
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 491307756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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