Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP12_F_I12
(945 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 42 1e-04
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 34 0.025
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 34 0.025
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 33 0.059
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 0.096
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 32 0.14
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.55
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 28 1.7
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 28 2.2
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 26 6.7
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 6.7
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 8.9
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 42.3 bits (95), Expect = 1e-04
Identities = 25/59 (42%), Positives = 25/59 (42%), Gaps = 3/59 (5%)
Frame = -1
Query: 711 PXPPPPXXXGWGXXXAP---PXPQXFXGXXPPPPWGGXXXPPPXXGGXXPXPPPXXPPP 544
P PPPP AP P P G PPPP PPP G P PPP PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPP------PPPGVAGAGP-PPPPPPPP 783
Score = 33.9 bits (74), Expect = 0.034
Identities = 12/18 (66%), Positives = 12/18 (66%)
Frame = -2
Query: 599 PPPXXAGXXPPPPPXXPP 546
PPP AG PPPPP PP
Sbjct: 766 PPPGVAGAGPPPPPPPPP 783
Score = 30.3 bits (65), Expect = 0.41
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = -2
Query: 944 PPPXGXPPXGXGXXPPPPP 888
PPP PP G PPPPP
Sbjct: 761 PPPPPPPPGVAGAGPPPPP 779
Score = 27.9 bits (59), Expect = 2.2
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = -2
Query: 944 PPPXGXPPXGXGXXPPPPPGXXXXXPXXXP 855
P P P G PPPPPG P P
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
Score = 27.5 bits (58), Expect = 2.9
Identities = 19/62 (30%), Positives = 19/62 (30%)
Frame = -2
Query: 944 PPPXGXPPXGXGXXPPPPPGXXXXXPXXXPGXKRGPXRGXXGGXPPXFFXPGGXPPPXXG 765
PP P P PPP P P P G G PP P PPP
Sbjct: 736 PPAVIVPTPAPAPIPVPPPAPIMGGPPPPP-----PPPGVAGAGPP----PPPPPPPAVS 786
Query: 764 XG 759
G
Sbjct: 787 AG 788
Score = 27.1 bits (57), Expect = 3.8
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = -2
Query: 599 PPPXXAGXXPPPPPXXPPPXXXXXG 525
PPP PPPPP PPP G
Sbjct: 752 PPPAPIMGGPPPPP--PPPGVAGAG 774
Score = 26.2 bits (55), Expect = 6.7
Identities = 15/45 (33%), Positives = 15/45 (33%), Gaps = 2/45 (4%)
Frame = -2
Query: 902 PPPPPGXXXXXPXXXP--GXKRGPXRGXXGGXPPXFFXPGGXPPP 774
PPPPP P P P G PP G PPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 34.3 bits (75), Expect = 0.025
Identities = 21/66 (31%), Positives = 21/66 (31%), Gaps = 3/66 (4%)
Frame = -1
Query: 735 GGGXXXXXPXPPPPXXXGWGXXXAPPXPQXFXGXXPPPPWGG---XXXPPPXXGGXXPXP 565
G P PPPP G P PQ PPPP PPP
Sbjct: 330 GSSNSSLPPPPPPPRSNAAGSIPLP--PQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSN 387
Query: 564 PPXXPP 547
PP PP
Sbjct: 388 PPAPPP 393
Score = 31.1 bits (67), Expect = 0.24
Identities = 31/130 (23%), Positives = 32/130 (24%), Gaps = 2/130 (1%)
Frame = -2
Query: 926 PPXGXGXXPPPPPGXXXXXPXXXPGXKRGPXRGXXGGXPPXFFXPGGXPPPXXGXGVFFK 747
PP G PPPPP P PP PG P G +
Sbjct: 354 PPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAI--PGRSAPALPPLGNASR 411
Query: 746 XXXXXXGXXXKXPXXPPPXXGXGXPXXPPXGHXFFXXXXXXXXXGXXXXPPPXXAG--XX 573
P PP P P G PP AG
Sbjct: 412 TSTPPVPTPPSLPPSAPPSLPPSAPPSLPMG-----APAAPPLPPSAPIAPPLPAGMPAA 466
Query: 572 PPPPPXXPPP 543
PP PP P P
Sbjct: 467 PPLPPAAPAP 476
Score = 28.7 bits (61), Expect = 1.3
Identities = 18/59 (30%), Positives = 18/59 (30%), Gaps = 3/59 (5%)
Frame = -1
Query: 711 PXPPPPXXXGWGXXXAPPXPQXFXGXXPPPPW---GGXXXPPPXXGGXXPXPPPXXPPP 544
P PPPP G PPPP P P G P PPP P
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAP 370
Score = 28.3 bits (60), Expect = 1.7
Identities = 17/53 (32%), Positives = 17/53 (32%)
Frame = -1
Query: 705 PPPPXXXGWGXXXAPPXPQXFXGXXPPPPWGGXXXPPPXXGGXXPXPPPXXPP 547
P P G APP P PP P G PP P P P P
Sbjct: 433 PSAPPSLPMGAPAAPPLPPSAP-IAPPLPAGMPAAPPLPPAAPAPPPAPAPAP 484
Score = 25.8 bits (54), Expect = 8.9
Identities = 19/60 (31%), Positives = 19/60 (31%), Gaps = 4/60 (6%)
Frame = -1
Query: 711 PXPPPPXXXGWGXXXA----PPXPQXFXGXXPPPPWGGXXXPPPXXGGXXPXPPPXXPPP 544
P PPP A PP P PPP PP G PP PPP
Sbjct: 291 PLPPPSSRVSAAALAANKKRPPPP-------PPPSRRNRGKPPIGNGSSNSSLPPPPPPP 343
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 34.3 bits (75), Expect = 0.025
Identities = 20/55 (36%), Positives = 21/55 (38%), Gaps = 1/55 (1%)
Frame = -1
Query: 705 PPPPXXXGWGXXXAPPXPQXFXGXXP-PPPWGGXXXPPPXXGGXXPXPPPXXPPP 544
PP P G APP P+ P P P G P G P PPP PP
Sbjct: 1150 PPVPAPSG-----APPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPP 1199
Score = 33.1 bits (72), Expect = 0.059
Identities = 18/69 (26%), Positives = 22/69 (31%)
Frame = +3
Query: 729 PPPGXTLKKNPXPXXGGGXPPXVKKXRGXPPXXTPXGPPFXPRXXXXXXXPXPRGGGGXL 908
P P ++ P P G PP K G PP P P P+ P +
Sbjct: 1160 PVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPV 1219
Query: 909 XXPXGGXSP 935
P G P
Sbjct: 1220 PTPSAGLPP 1228
Score = 33.1 bits (72), Expect = 0.059
Identities = 15/40 (37%), Positives = 16/40 (40%)
Frame = -1
Query: 663 PPXPQXFXGXXPPPPWGGXXXPPPXXGGXXPXPPPXXPPP 544
PP P+ G P PP P G P PPP PP
Sbjct: 1179 PPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPP 1218
Score = 30.7 bits (66), Expect = 0.31
Identities = 20/70 (28%), Positives = 24/70 (34%), Gaps = 1/70 (1%)
Frame = +3
Query: 729 PPPGXTLKKNPXPXXGGGXPPXVKKXRGXPPXXTPXGPPFXPR-XXXXXXXPXPRGGGGX 905
P P ++ P P G PP K PP P G P P+ P P G
Sbjct: 1122 PVPKPSVAAPPVPVPSGA-PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPP 1180
Query: 906 LXXPXGGXSP 935
+ P G P
Sbjct: 1181 VPKPAAGVPP 1190
Score = 29.1 bits (62), Expect = 0.95
Identities = 17/57 (29%), Positives = 17/57 (29%), Gaps = 1/57 (1%)
Frame = -2
Query: 944 PPPXGXPPXGX-GXXPPPPPGXXXXXPXXXPGXKRGPXRGXXGGXPPXFFXPGGXPP 777
P P G PP PP P P P P G PP G PP
Sbjct: 1134 PVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPP 1190
Score = 29.1 bits (62), Expect = 0.95
Identities = 14/41 (34%), Positives = 15/41 (36%)
Frame = -1
Query: 666 APPXPQXFXGXXPPPPWGGXXXPPPXXGGXXPXPPPXXPPP 544
APP P+ G P PP P G P P P P
Sbjct: 1197 APPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAP 1237
Score = 27.9 bits (59), Expect = 2.2
Identities = 19/55 (34%), Positives = 20/55 (36%), Gaps = 1/55 (1%)
Frame = -1
Query: 705 PPPPXXXGWGXXXAPPXPQXFXGXXPPP-PWGGXXXPPPXXGGXXPXPPPXXPPP 544
PP P G APP P+ P P P G P P P P P PP
Sbjct: 1112 PPVPAPSG-----APPVPKPSVAAPPVPVPSGAPPVPKPSVAAP-PVPAPSGAPP 1160
Score = 27.9 bits (59), Expect = 2.2
Identities = 18/46 (39%), Positives = 18/46 (39%), Gaps = 5/46 (10%)
Frame = -1
Query: 666 APPXPQXFXGXXP-PPPWGG-XXXPPPXXGGXXPXPP---PXXPPP 544
APP P G P P P G PPP P P P PPP
Sbjct: 1168 APPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPP 1213
Score = 26.6 bits (56), Expect = 5.1
Identities = 15/42 (35%), Positives = 16/42 (38%), Gaps = 1/42 (2%)
Frame = -1
Query: 666 APPXPQXFXGXXP-PPPWGGXXXPPPXXGGXXPXPPPXXPPP 544
APP P+ P P P G P P P P P PP
Sbjct: 1101 APPVPKPSVAVPPVPAPSGAPPVPKPSVAAP-PVPVPSGAPP 1141
Score = 26.6 bits (56), Expect = 5.1
Identities = 14/43 (32%), Positives = 17/43 (39%)
Frame = +3
Query: 729 PPPGXTLKKNPXPXXGGGXPPXVKKXRGXPPXXTPXGPPFXPR 857
P P ++ P P G PP K PP P G P P+
Sbjct: 1103 PVPKPSVAVPPVPAPSGA-PPVPKPSVAAPPVPVPSGAPPVPK 1144
Score = 25.8 bits (54), Expect = 8.9
Identities = 14/41 (34%), Positives = 14/41 (34%)
Frame = -1
Query: 666 APPXPQXFXGXXPPPPWGGXXXPPPXXGGXXPXPPPXXPPP 544
APP P PP P P P P PP P P
Sbjct: 1031 APPIP--VPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAP 1069
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/54 (29%), Positives = 17/54 (31%)
Frame = -1
Query: 705 PPPPXXXGWGXXXAPPXPQXFXGXXPPPPWGGXXXPPPXXGGXXPXPPPXXPPP 544
PP P G PP P+ P P P P G P P P P
Sbjct: 1083 PPVPAPSG-----IPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAP 1131
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 33.1 bits (72), Expect = 0.059
Identities = 21/56 (37%), Positives = 22/56 (39%)
Frame = +2
Query: 545 GGGXWGGGXGXXPPXXGGGXXXPPQGGGGXXPXKXCGXGGAXXXPXPXXXGGGGXG 712
G G +GGG G PP GG GG G G GG P G GG G
Sbjct: 189 GFGGFGGGSGGPPPGPGGFGGF---GGFGGEGHHHGGHGGFGGGPGGFEGGPGGFG 241
Score = 25.8 bits (54), Expect = 8.9
Identities = 19/56 (33%), Positives = 20/56 (35%)
Frame = +2
Query: 545 GGGXWGGGXGXXPPXXGGGXXXPPQGGGGXXPXKXCGXGGAXXXPXPXXXGGGGXG 712
G G +GGG G GG P GGG G GG G GG G
Sbjct: 222 GHGGFGGGPGGFEGGPGGFGGGPGGFGGGLG-----GFGGGPGGFGGGPGGHGGPG 272
Score = 25.8 bits (54), Expect = 8.9
Identities = 13/31 (41%), Positives = 13/31 (41%)
Frame = +1
Query: 544 GGGXXGGGGGXXPAXXGGGXXXXPPRGGGXP 636
GGG G GGG G G P G G P
Sbjct: 241 GGGPGGFGGGLGGFGGGPGGFGGGPGGHGGP 271
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 26.6 bits (56), Expect(2) = 0.096
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = -3
Query: 568 PPPPXPPPPXXXXXG 524
PPPP PPPP G
Sbjct: 945 PPPPPPPPPLVSAAG 959
Score = 24.2 bits (50), Expect(2) = 0.096
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = -3
Query: 574 PXPPPPXPPPP 542
P PPP PPPP
Sbjct: 942 PAFPPPPPPPP 952
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.9 bits (69), Expect = 0.14
Identities = 22/63 (34%), Positives = 22/63 (34%)
Frame = -1
Query: 732 GGXXXXXPXPPPPXXXGWGXXXAPPXPQXFXGXXPPPPWGGXXXPPPXXGGXXPXPPPXX 553
GG P PP A P PQ PPPP PPP P PP
Sbjct: 1679 GGMAPAHPVSTPPVRP----QSAAP-PQMSAPTPPPPPMS---VPPPPSAPPMPAGPPSA 1730
Query: 552 PPP 544
PPP
Sbjct: 1731 PPP 1733
Score = 26.2 bits (55), Expect = 6.7
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = -1
Query: 711 PXPPPPXXXGWGXXXAPPXPQXFXGXXPPP 622
P PPPP APP P PPP
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.9 bits (64), Expect = 0.55
Identities = 12/26 (46%), Positives = 12/26 (46%)
Frame = -1
Query: 621 PWGGXXXPPPXXGGXXPXPPPXXPPP 544
P G PPP G P PP PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 27.1 bits (57), Expect = 3.8
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = -2
Query: 944 PPPXGXPPXGXGXXPPPPPG 885
PPP G P PPPPPG
Sbjct: 13 PPPPGFEPPSQ-PPPPPPPG 31
Score = 23.4 bits (48), Expect(2) = 7.3
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = -2
Query: 923 PXGXGXXPPPPPG 885
P G PPPPPG
Sbjct: 5 PPGNPPPPPPPPG 17
Score = 20.6 bits (41), Expect(2) = 7.3
Identities = 8/18 (44%), Positives = 8/18 (44%)
Frame = -2
Query: 812 PPXFFXPGGXPPPXXGXG 759
PP F P PPP G
Sbjct: 14 PPPGFEPPSQPPPPPPPG 31
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 28.3 bits (60), Expect = 1.7
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = -2
Query: 599 PPPXXAGXXPPPPPXXPPP 543
P P G PPPPP P P
Sbjct: 347 PTPQVQGSRPPPPPPMPAP 365
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 27.9 bits (59), Expect = 2.2
Identities = 21/61 (34%), Positives = 21/61 (34%), Gaps = 8/61 (13%)
Frame = -1
Query: 705 PPPPXXXGWGXXXAPPXPQXFXG-XXPPPPW---GGXXXPPP----XXGGXXPXPPPXXP 550
PPPP G PP G PPPP G PPP G P PP
Sbjct: 208 PPPPMHHKPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHE 267
Query: 549 P 547
P
Sbjct: 268 P 268
Score = 27.9 bits (59), Expect = 2.2
Identities = 21/61 (34%), Positives = 21/61 (34%), Gaps = 8/61 (13%)
Frame = -1
Query: 705 PPPPXXXGWGXXXAPPXPQXFXG-XXPPPPW---GGXXXPPP----XXGGXXPXPPPXXP 550
PPPP G PP G PPPP G PPP G P PP
Sbjct: 247 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHE 306
Query: 549 P 547
P
Sbjct: 307 P 307
Score = 27.1 bits (57), Expect = 3.8
Identities = 20/56 (35%), Positives = 20/56 (35%), Gaps = 8/56 (14%)
Frame = -1
Query: 705 PPPPXXXGWGXXXAPPXPQXFXG-XXPPPPW---GGXXXPPP----XXGGXXPXPP 562
PPPP G PP G PPPP G PPP G P PP
Sbjct: 286 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 341
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 26.2 bits (55), Expect = 6.7
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -3
Query: 568 PPPPXPPPP 542
PPPP PPPP
Sbjct: 306 PPPPPPPPP 314
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.2 bits (55), Expect = 6.7
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -3
Query: 568 PPPPXPPPP 542
PPPP PPPP
Sbjct: 189 PPPPPPPPP 197
Score = 22.2 bits (45), Expect(2) = 7.7
Identities = 8/16 (50%), Positives = 8/16 (50%)
Frame = -2
Query: 593 PXXAGXXPPPPPXXPP 546
P A P PPP PP
Sbjct: 229 PKQADPLPAPPPPPPP 244
Score = 21.8 bits (44), Expect(2) = 7.7
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = -2
Query: 572 PPPPPXXPPP 543
PPPPP PP
Sbjct: 239 PPPPPPTLPP 248
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/55 (29%), Positives = 16/55 (29%), Gaps = 1/55 (1%)
Frame = -1
Query: 705 PPPPXXXGWGXXXAPPXPQXFXGXXPPP-PWGGXXXPPPXXGGXXPXPPPXXPPP 544
PP P APP P P P P P PP PPP
Sbjct: 151 PPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPP 205
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,007,644
Number of Sequences: 5004
Number of extensions: 36084
Number of successful extensions: 337
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 42
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 481321826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -