Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP12_F_H21
(880 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 47 4e-06
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 43 5e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.010
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 32 0.094
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 31 0.22
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 30 0.50
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 29 0.66
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 1.5
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 2.7
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 2.7
SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 27 3.5
SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 27 4.7
SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit L37|... 27 4.7
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 26 8.1
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 46.8 bits (106), Expect = 4e-06
Identities = 25/89 (28%), Positives = 46/89 (51%), Gaps = 1/89 (1%)
Frame = +3
Query: 399 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSES 578
D DG+ + E ++ G +E +++ ++ D D +G+I EFL + M ++
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDT 81
Query: 579 -RRNIVEQAFKKLDKTGDGAITIDDIKGV 662
V +AFK DK G+G IT++++ V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHV 110
Score = 37.1 bits (82), Expect = 0.003
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +3
Query: 387 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 545
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 43.2 bits (97), Expect = 5e-05
Identities = 28/107 (26%), Positives = 53/107 (49%), Gaps = 6/107 (5%)
Frame = +3
Query: 381 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEEL-FSQFDTDSSGSISLDEFLIKI 557
R+F +D+DG ++ +EF+ + + NK E + F +D D G IS E + +
Sbjct: 60 RLFSVVDEDGGGDVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVL 119
Query: 558 RPPMSESRR-----NIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQT 683
+ + + R IV++ ++DK DG I+ ++ K + S + T
Sbjct: 120 KMMVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKDIVSGSNVT 166
Score = 27.5 bits (58), Expect = 2.7
Identities = 23/86 (26%), Positives = 39/86 (45%), Gaps = 1/86 (1%)
Frame = +3
Query: 387 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRP- 563
F ++D + S +++ EFL I + A LFS D D G + EF+ +
Sbjct: 30 FIKIDANQSGSIDRNEFL-SIPSVA---SNPLASRLFSVVDEDGGGDVDFQEFINSLSVF 85
Query: 564 PMSESRRNIVEQAFKKLDKTGDGAIT 641
+ ++ ++ AFK D DG I+
Sbjct: 86 SVHGNKEEKLKFAFKIYDIDRDGYIS 111
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.5 bits (78), Expect = 0.010
Identities = 18/97 (18%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
Frame = +3
Query: 375 LGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL-I 551
+ F+ D D ++ E ++ G KSE ++ FD G + +++F+ +
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 552 KIRPPMSESRRNIVEQAFKKLDKTGDGAITIDDIKGV 662
+ +++AF+ D G I++ +++ V
Sbjct: 99 MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRV 135
Score = 33.1 bits (72), Expect = 0.054
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +3
Query: 381 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 548
R F DDD + ++ KE ++ E E + +FD D G I+ EF+
Sbjct: 114 RAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
Score = 32.7 bits (71), Expect = 0.071
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = +3
Query: 465 ELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 644
E + + E F FD+D +I E +R + ++ V + + DKTG G + +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 645 DD 650
+D
Sbjct: 93 ED 94
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 32.3 bits (70), Expect = 0.094
Identities = 23/100 (23%), Positives = 42/100 (42%), Gaps = 2/100 (2%)
Frame = +3
Query: 360 QGILGLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLD 539
Q + G+ ++FR + S +LN+ EF + GL + E LF + G ++ +
Sbjct: 484 QELDGITKVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG-VTYE 542
Query: 540 EFLIKIRPPMSESRRNIVEQAFKKLDKTGDGA--ITIDDI 653
F + + + R+ Q DG +T DD+
Sbjct: 543 RFTEIVMEELED--RDSARQVLYAFCDVADGKSYVTSDDL 580
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 31.1 bits (67), Expect = 0.22
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 1/79 (1%)
Frame = +3
Query: 420 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVE 596
LNK EF K+ + S AE +F+ FD D +G I EF+ + + +
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLI 102
Query: 597 QAFKKLDKTGDGAITIDDI 653
AF+ D +G I+ D++
Sbjct: 103 WAFQLYDLDNNGLISYDEM 121
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 29.9 bits (64), Expect = 0.50
Identities = 18/74 (24%), Positives = 31/74 (41%)
Frame = +3
Query: 432 EFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKK 611
E+ G+ + + S LF +FD +GS+SL + + I + F+
Sbjct: 564 EWAKGLDAAAINNSSSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISFIFEL 623
Query: 612 LDKTGDGAITIDDI 653
D GDG + D+
Sbjct: 624 YDFNGDGFMDKPDV 637
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 29.5 bits (63), Expect = 0.66
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +1
Query: 475 KVKPKNSSVNSTQTVVAQSVLMNSLLKSVLPCRNRVVT-L*NKHSRSLTRLVTVQSQL 645
K+ KN+ S + + ++ L L L + +++ L NK S TRL +QSQL
Sbjct: 765 KLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQL 822
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 28.3 bits (60), Expect = 1.5
Identities = 23/80 (28%), Positives = 39/80 (48%), Gaps = 2/80 (2%)
Frame = +3
Query: 462 LELNKSEA--EELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGA 635
L LN S EE F + D D SG +S +EF + + ++R IV+ FK+ +G
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEFQHFV--SLLKTRSEIVD-IFKEY-TSGSDK 381
Query: 636 ITIDDIKGVYSVDSQTRYXS 695
++++ + S + R S
Sbjct: 382 MSLEQFRHFLSTSQKARLDS 401
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 27.5 bits (58), Expect = 2.7
Identities = 22/71 (30%), Positives = 32/71 (45%)
Frame = +3
Query: 435 FLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKL 614
F+ +E+ LE E FSQ +S + SL+ I P SRR IVEQ ++
Sbjct: 680 FILPNEESLLEKYWINYNESFSQLSRESLFT-SLESPFTDIESPTIVSRRKIVEQRKLRM 738
Query: 615 DKTGDGAITID 647
+K +D
Sbjct: 739 EKESFQETNVD 749
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 27.5 bits (58), Expect = 2.7
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +3
Query: 567 MSESRRNIVEQAFKKLDKTGDGAITIDDIK 656
++ S+ +++AF LDK GDG I +D+K
Sbjct: 42 LTSSQIQELKEAFALLDKDGDGNIGREDVK 71
>SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 488
Score = 27.1 bits (57), Expect = 3.5
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 206 KPKTSSNIAWHRPMSAGSVQEEELMQK 286
KPK S ++W+ + GS +E EL+ K
Sbjct: 56 KPKKDSLLSWNILLKKGSYKENELLAK 82
>SPCC622.12c |||NADP-specific glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 451
Score = 26.6 bits (56), Expect = 4.7
Identities = 12/41 (29%), Positives = 18/41 (43%), Gaps = 2/41 (4%)
Frame = +1
Query: 166 GCNVILNSKNVFRKTE--NIFQHCVAQTDVCWFRPGGRADA 282
GC + N+ E +F+ A + CW PG A+A
Sbjct: 331 GCRYVAEGSNMGSSAEAVEVFEKSRASGEGCWLAPGKAANA 371
>SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit
L37|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 26.6 bits (56), Expect = 4.7
Identities = 16/48 (33%), Positives = 23/48 (47%)
Frame = +1
Query: 424 TKKNSFMASRKQGWNLIKVKPKNSSVNSTQTVVAQSVLMNSLLKSVLP 567
+ +NS + K+ + +V PK NS T AQ V + SVLP
Sbjct: 32 SSRNSSSSLVKRSYVSSRVSPKKPQHNSDATSSAQKVANKTHTSSVLP 79
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/40 (27%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = -2
Query: 444 HKGIL-LCSVVYFHHHPCDGKFFQVPRSPEAPRDKQSKRS 328
H+G++ +C++VY KF + P++ E R +K++
Sbjct: 686 HRGLVCICNIVYSKDQEIFNKFIKTPKAVETLRTYITKQA 725
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,917,516
Number of Sequences: 5004
Number of extensions: 55537
Number of successful extensions: 163
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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