Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP12_F_D08
(872 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 53 6e-08
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 38 0.002
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 32 0.093
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 29 0.86
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met... 27 4.6
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 4.6
SPBC32H8.09 |||WD repeat protein, human WDR8 family|Schizosaccha... 26 6.1
SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyce... 26 8.1
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 26 8.1
SPBC27.08c |sua1|SPBC28F2.01c|sulfate adenylyltransferase |Schiz... 26 8.1
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 26 8.1
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 52.8 bits (121), Expect = 6e-08
Identities = 29/87 (33%), Positives = 50/87 (57%)
Frame = +2
Query: 437 NIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEK 616
N+I F EL + C + + AE A +GP+ + LA KY + I+ E++EK
Sbjct: 39 NLILFPELITSGYE-CGNT---FTQIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEK 94
Query: 617 HSDILWNTAVVISDTGNVIGKHRKNHI 697
S+I++N+ + I++ GN+ G +RK H+
Sbjct: 95 QSNIIYNSCIYITENGNLGGVYRKVHL 121
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 37.5 bits (83), Expect = 0.002
Identities = 34/130 (26%), Positives = 60/130 (46%)
Frame = +2
Query: 308 RIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCT 487
R ++G++Q +A D+ N Q + K+++ A + G N+I E++N P+ T
Sbjct: 42 RAFRIGLVQ--LANTKDKSENLQLARL-----KVLEAA-KNGSNVIVLPEIFNSPYG--T 91
Query: 488 REKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 667
+ E E E P+ L +A + + E+ L+NTA+V +G
Sbjct: 92 GYFNQYAEPIE--ESSPSYQALSSMAKDTKTYLFGGSIP--ERKDGKLYNTAMVFDPSGK 147
Query: 668 VIGKHRKNHI 697
+I HRK H+
Sbjct: 148 LIAVHRKIHL 157
>SPBC336.05c |||S-adenosylmethionine-
dependentmethyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 378
Score = 32.3 bits (70), Expect = 0.093
Identities = 21/64 (32%), Positives = 31/64 (48%)
Frame = -3
Query: 549 KVVVGPSSADSANSHHGCFSLVQNAKGIFHNSWKQMMLTPSWPATSMIFLTLLKIAFFCS 370
++ V S ++A SH CF QN+ + + + +P T F+ LLK AFF
Sbjct: 232 QIAVFHQSKNNAASH--CFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDH 289
Query: 369 LTGR 358
L GR
Sbjct: 290 LFGR 293
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 29.1 bits (62), Expect = 0.86
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = +2
Query: 521 SAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDIL 631
SAE+ + + + +++VS++LE DEKH D++
Sbjct: 979 SAENTTSFSIFAAQGLTDFLIVVSNLLEMDEKHVDVV 1015
>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
Met11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 26.6 bits (56), Expect = 4.6
Identities = 15/55 (27%), Positives = 27/55 (49%)
Frame = -2
Query: 436 NTLLAGNIDDLLDFIENCFLLLVDWTIGGHRDGMLNYSYLDNSRGSGLLVLGRES 272
N ++ + + D N F+ ++ GH+D + + S GSG VLGR++
Sbjct: 341 NQVVVPSQQPVADKSSNLFVTSKQSSVSGHKDNLTEEAPFSVSEGSG--VLGRQA 393
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 26.6 bits (56), Expect = 4.6
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = -2
Query: 400 DFIENCFLLLVDWTIGGHRDGMLNYSYLDNSRGSGLLVLGRESVCGDVEVSL 245
+FI+ CF + + GH D +++ S +S GS +G S D++VSL
Sbjct: 661 EFIQRCFHFADEASPDGHSDTLIDISDHMSSTGSENRSVGANS---DIKVSL 709
>SPBC32H8.09 |||WD repeat protein, human WDR8
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 483
Score = 26.2 bits (55), Expect = 6.1
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -1
Query: 74 LRKLXLPTXKS*GIPYSVNGNW 9
L++ LPT S GI +S +GNW
Sbjct: 182 LKECKLPTIDSTGIHWSPDGNW 203
>SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +1
Query: 661 RKRDRKTSQEPHS*KSAILTNPTTTWKVT 747
RKR++KT E H + + L W+VT
Sbjct: 136 RKREKKTEIEKHLERISFLNKVPENWQVT 164
>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 502
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 139 NNLTGRDLEEFNRIHFGRRNNLEI 210
NN+ R +EE N I G+R LE+
Sbjct: 8 NNILKRHIEEDNNIDNGKRKKLEL 31
>SPBC27.08c |sua1|SPBC28F2.01c|sulfate adenylyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 490
Score = 25.8 bits (54), Expect = 8.1
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = -1
Query: 791 HRDLAISGRKYRMAGVTFHVVVGFVKIAD 705
HR+L + K A V H VVG K D
Sbjct: 202 HRELTVRAAKQHGARVLIHPVVGMTKPGD 230
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +1
Query: 109 ETHSLESII-NNNLTGRDLEEFNRIHFGRRNNLEIKLK 219
E H +E +I + N+T DL F + FG+ N ++ K
Sbjct: 369 EFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFK 406
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,526,617
Number of Sequences: 5004
Number of extensions: 73052
Number of successful extensions: 222
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 208
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 221
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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