Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP10_F_D04
         (884 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q9Y113 Cluster: Negative elongation factor B homolog; n...   179   1e-43
UniRef50_UPI0000E4898B Cluster: PREDICTED: hypothetical protein;...   122   9e-27
UniRef50_Q8WX92 Cluster: Negative elongation factor B; n=64; Eut...    99   7e-20
UniRef50_A7T0W2 Cluster: Predicted protein; n=1; Nematostella ve...    92   1e-17
UniRef50_Q54Q08 Cluster: Putative uncharacterized protein; n=1; ...    37   0.59 

>UniRef50_Q9Y113 Cluster: Negative elongation factor B homolog; n=6;
           Endopterygota|Rep: Negative elongation factor B homolog
           - Drosophila melanogaster (Fruit fly)
          Length = 594

 Score =  179 bits (435), Expect = 1e-43
 Identities = 97/193 (50%), Positives = 118/193 (61%)
 Frame = +2

Query: 188 GTGLEEVGVPGQTFLRDALTSCTDPLKAIEEFQLENGIXXXXXXXXXXXXXXHGVRRLDF 367
           GTGLE+V +PGQ +LR+ALTSCTDPLKAIE FQLENG+              HGVRRLDF
Sbjct: 12  GTGLEDVNIPGQAYLREALTSCTDPLKAIESFQLENGVLLPSLRPMLPLLDLHGVRRLDF 71

Query: 368 HTSVLEELREKLIAHIXXXXXXXXXXXXXXXXXXXXXXFPVVRVKALRPVIMGILKNIPH 547
           HTS++EELR+KLIAHI                      FPVVRVK+LRPV+M IL+N  H
Sbjct: 72  HTSLMEELRDKLIAHINEMGQKEPRERDKKLKELLVKSFPVVRVKSLRPVVMAILRNTQH 131

Query: 548 IDDKYLRVLVRDRELYNDTDTEGEASNMARQPVTVWR*SFSTPQSVHQGXKKMFYLIMKI 727
           IDDKYL++LVRDRELY DTDTE +   + R   +++    S   S +   K+        
Sbjct: 132 IDDKYLKILVRDRELYADTDTEVK-RQIWRDNQSLFGDEVSPLLSQYIREKEHILFDHTN 190

Query: 728 SLIYFFPPSPKVR 766
               FF P+PKVR
Sbjct: 191 LNNLFFHPTPKVR 203



 Score = 68.9 bits (161), Expect = 2e-10
 Identities = 39/67 (58%), Positives = 44/67 (65%)
 Frame = +3

Query: 615 VKRQIWQDNQSLFGDEVSPLLSQYIRXXRRCSI*S*KSH*SIFFPLHQK*EXXGAVVQXL 794
           VKRQIW+DNQSLFGDEVSPLLSQYIR            + ++FF    K    G VVQ L
Sbjct: 154 VKRQIWRDNQSLFGDEVSPLLSQYIREKEHILFDHTNLN-NLFFHPTPKVRRQGEVVQKL 212

Query: 795 AHMIGTS 815
           A+MIGTS
Sbjct: 213 ANMIGTS 219


>UniRef50_UPI0000E4898B Cluster: PREDICTED: hypothetical protein;
           n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
           hypothetical protein - Strongylocentrotus purpuratus
          Length = 640

 Score =  122 bits (295), Expect = 9e-27
 Identities = 61/150 (40%), Positives = 84/150 (56%)
 Frame = +2

Query: 194 GLEEVGVPGQTFLRDALTSCTDPLKAIEEFQLENGIXXXXXXXXXXXXXXHGVRRLDFHT 373
           G EEVG+ G   LR+ALT+CTDPL+AIE+FQ ENGI              H ++R  FH 
Sbjct: 3   GFEEVGIAGSVHLREALTNCTDPLQAIEDFQSENGILLPTLQPALPLLDLHMIQRFAFHH 62

Query: 374 SVLEELREKLIAHIXXXXXXXXXXXXXXXXXXXXXXFPVVRVKALRPVIMGILKNIPHID 553
           S++EELREKL++                        FP++RVK+++PV+M ++K +P I 
Sbjct: 63  SIMEELREKLMSRAQELAKSDTKGRFKTIEDLLSKCFPLIRVKSIQPVVMSVMKCLPKIP 122

Query: 554 DKYLRVLVRDRELYNDTDTEGEASNMARQP 643
           +KYL VLV DR LY+    E +     R P
Sbjct: 123 EKYLTVLVEDRSLYDVASIEVKQQIWQRNP 152



 Score = 58.4 bits (135), Expect = 2e-07
 Identities = 32/69 (46%), Positives = 40/69 (57%)
 Frame = +3

Query: 609 LRVKRQIWQDNQSLFGDEVSPLLSQYIRXXRRCSI*S*KSH*SIFFPLHQK*EXXGAVVQ 788
           + VK+QIWQ N SLFGDEVSPLL+ YI+        S   +  +FF +  K    G VVQ
Sbjct: 141 IEVKQQIWQRNPSLFGDEVSPLLTDYIKNKESVLFGSEAVNSQMFFLVSPKMRRQGHVVQ 200

Query: 789 XLAHMIGTS 815
            L  M+G S
Sbjct: 201 KLTGMVGKS 209


>UniRef50_Q8WX92 Cluster: Negative elongation factor B; n=64;
           Euteleostomi|Rep: Negative elongation factor B - Homo
           sapiens (Human)
          Length = 580

 Score =   99 bits (238), Expect = 7e-20
 Identities = 58/193 (30%), Positives = 101/193 (52%), Gaps = 2/193 (1%)
 Frame = +2

Query: 194 GLEEVGVPGQTFLRDALTSCTDPLKAIEEFQLENGIXXXXXXXXXXXXXXHGVRRLDFHT 373
           GL+++GV     L++ LT+CT+PLKAIE+FQ ENG+              HG  RL+FH 
Sbjct: 4   GLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQ 63

Query: 374 SVLEELREKLIAHI-XXXXXXXXXXXXXXXXXXXXXXFPVVRVKALRPVIMGILKNIPHI 550
           SV +ELR+KL+  +                       F +V++ +L+PV+M ++K++P +
Sbjct: 64  SVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHLPKV 123

Query: 551 DDKYLRVLVRDRELYNDTDTEGEASNMARQPVTVWR*SFSTPQSVHQGXKKMFYLIMKIS 730
            +K L++++ D+ELY     E +   + +    ++    S     +   K+      ++S
Sbjct: 124 PEKKLKLVMADKELYRACAVEVK-RQIWQDNQALFGDEVSPLLKQYILEKESALFSTELS 182

Query: 731 LIY-FFPPSPKVR 766
           +++ FF PSPK R
Sbjct: 183 VLHNFFSPSPKTR 195


>UniRef50_A7T0W2 Cluster: Predicted protein; n=1; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 577

 Score = 92.3 bits (219), Expect = 1e-17
 Identities = 50/140 (35%), Positives = 71/140 (50%)
 Frame = +2

Query: 194 GLEEVGVPGQTFLRDALTSCTDPLKAIEEFQLENGIXXXXXXXXXXXXXXHGVRRLDFHT 373
           G E  G+PG   LR AL++ +D    IE FQ +NGI              HGVRRLDFH+
Sbjct: 3   GFERAGLPGIEHLRQALSNSSDLAATIESFQHDNGILLPSLQSALPFLDLHGVRRLDFHS 62

Query: 374 SVLEELREKLIAHIXXXXXXXXXXXXXXXXXXXXXXFPVVRVKALRPVIMGILKNIPHID 553
           SV+E LRE ++  +                      FP + VK LRPV++ ++K++P I+
Sbjct: 63  SVMEHLRESVLEKV-------KDLSTQKLEEILEQSFPFIHVKELRPVVVAVMKHLPKIN 115

Query: 554 DKYLRVLVRDRELYNDTDTE 613
           D+ L  +  D +LY D   E
Sbjct: 116 DECLEHIANDAKLYEDCPIE 135



 Score = 52.0 bits (119), Expect = 2e-05
 Identities = 32/70 (45%), Positives = 41/70 (58%), Gaps = 1/70 (1%)
 Frame = +3

Query: 609 LRVKRQIWQDNQSLFGDEVSPLLSQYIRXXRRCSI*S*KSH*S-IFFPLHQK*EXXGAVV 785
           + VKRQIW  +  LFGD V PLL+QYI   +   + S ++H S  FF +  K     A+V
Sbjct: 134 IEVKRQIWSKHHHLFGDAVGPLLNQYIE-AKYTLLHSTEAHNSHSFFSVPSKTRRQHAIV 192

Query: 786 QXLAHMIGTS 815
           Q LA MIG S
Sbjct: 193 QGLAKMIGKS 202


>UniRef50_Q54Q08 Cluster: Putative uncharacterized protein; n=1;
           Dictyostelium discoideum AX4|Rep: Putative
           uncharacterized protein - Dictyostelium discoideum AX4
          Length = 854

 Score = 37.1 bits (82), Expect = 0.59
 Identities = 14/27 (51%), Positives = 21/27 (77%)
 Frame = +3

Query: 609 LRVKRQIWQDNQSLFGDEVSPLLSQYI 689
           + VKRQIW  N+ LF +++ PL++QYI
Sbjct: 137 IEVKRQIWIVNEELFKEKIKPLINQYI 163


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 718,106,132
Number of Sequences: 1657284
Number of extensions: 11974322
Number of successful extensions: 25808
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 25085
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25794
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 79522270534
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)