Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP10_F_C20
(876 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 205 7e-54
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 196 3e-51
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 106 5e-24
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 95 1e-20
SPBC18H10.16 |||amino acid permease, unknown 9|Schizosaccharomyc... 29 1.1
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 205 bits (500), Expect = 7e-54
Identities = 89/122 (72%), Positives = 103/122 (84%)
Frame = +2
Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 325
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 326 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 505
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 506 RI 511
+I
Sbjct: 121 KI 122
Score = 72.9 bits (171), Expect = 5e-14
Identities = 31/56 (55%), Positives = 42/56 (75%)
Frame = +3
Query: 513 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVS 680
R++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVS
Sbjct: 123 RRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVS 178
Score = 56.8 bits (131), Expect = 4e-09
Identities = 27/45 (60%), Positives = 29/45 (64%)
Frame = +2
Query: 740 DCAFMVDNEXIYDIXXRXLXXXRPTYXXLNRXIGQIXSSXPASLR 874
DC FMVDNE YDI R L RP+Y LNR I Q+ SS ASLR
Sbjct: 199 DCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLR 243
Score = 29.5 bits (63), Expect = 0.66
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +1
Query: 688 VEPYNSILTTHTT 726
VEPYNS+LTTH T
Sbjct: 182 VEPYNSVLTTHAT 194
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 196 bits (479), Expect = 3e-51
Identities = 90/127 (70%), Positives = 105/127 (82%), Gaps = 5/127 (3%)
Frame = +2
Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 310
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 311 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 490
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 491 DLVLDRI 511
D VL+RI
Sbjct: 120 DSVLERI 126
Score = 74.1 bits (174), Expect = 2e-14
Identities = 31/56 (55%), Positives = 42/56 (75%)
Frame = +3
Query: 513 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVS 680
R++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVS
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVS 182
Score = 58.4 bits (135), Expect = 1e-09
Identities = 28/45 (62%), Positives = 29/45 (64%)
Frame = +2
Query: 740 DCAFMVDNEXIYDIXXRXLXXXRPTYXXLNRXIGQIXSSXPASLR 874
DC FMVDNE YDI R L RPTY LNR I Q+ SS ASLR
Sbjct: 203 DCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLR 247
Score = 29.5 bits (63), Expect = 0.66
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +1
Query: 688 VEPYNSILTTHTT 726
VEPYNS+LTTH T
Sbjct: 186 VEPYNSVLTTHAT 198
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 106 bits (254), Expect = 5e-24
Identities = 51/120 (42%), Positives = 69/120 (57%)
Frame = +2
Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 325
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 326 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 505
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Score = 28.7 bits (61), Expect = 1.1
Identities = 13/45 (28%), Positives = 19/45 (42%)
Frame = +2
Query: 740 DCAFMVDNEXIYDIXXRXLXXXRPTYXXLNRXIGQIXSSXPASLR 874
D F +DNE + I L P+Y LN + + + S R
Sbjct: 197 DETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFR 241
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 95.1 bits (226), Expect = 1e-20
Identities = 48/123 (39%), Positives = 75/123 (60%), Gaps = 2/123 (1%)
Frame = +2
Query: 149 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 328
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 329 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 502
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 503 DRI 511
D I
Sbjct: 120 DMI 122
Score = 29.1 bits (62), Expect = 0.87
Identities = 15/51 (29%), Positives = 23/51 (45%)
Frame = +3
Query: 522 ADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 674
AD L+GF + H L+ERL+ Y KK ++++P Q
Sbjct: 126 ADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176
>SPBC18H10.16 |||amino acid permease, unknown 9|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1050
Score = 28.7 bits (61), Expect = 1.1
Identities = 14/56 (25%), Positives = 26/56 (46%)
Frame = +3
Query: 618 SVDYGKKSKLEFAIYPAPQVSLPRRALQLYPHHPHNXXAXXTVLSWSTMXPSMTSA 785
S + K S F + +S+P ++ ++P + T L WST+ ++ SA
Sbjct: 224 SAIFAKASNALFVVIILSTISIPISSIFVHPFKDPSLLVHFTGLKWSTLMKNLASA 279
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.1
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 225 STASSLMARCPQTRPSGVETILSTLSSARPELAST 329
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 244 WPDAHRQDHRGWRRFFQHFLQR 309
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,362,068
Number of Sequences: 5004
Number of extensions: 68943
Number of successful extensions: 227
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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