Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP08_F_L20
         (840 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   181   1e-46
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   177   2e-45
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   107   1e-24
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    74   2e-14
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe...    28   1.4  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  181 bits (440), Expect = 1e-46
 Identities = 99/197 (50%), Positives = 121/197 (61%), Gaps = 2/197 (1%)
 Frame = +2

Query: 254 PQTRPSGVETILSTLSSXETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGK 433
           P+T     +   ST  S ETG GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGK
Sbjct: 38  PETASQNSDGGFSTFFS-ETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGK 96

Query: 434 EDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGT--ALGSLPY*W 607
           EDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FHSFGGGT    G+L    
Sbjct: 97  EDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLER 156

Query: 608 SVSPLTTARSLNWSSPSTPRLRFPLXVVEPYNSIXXXXXXXXXXXXCFHGRQ*XHL*HLP 787
                T    L +S    P++     VVEPYNS+             F            
Sbjct: 157 LAMEYTKKSKLQFSVYPAPQV--STSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICR 214

Query: 788 PXXDIXRPT*TNLNRLI 838
              DI RP+  NLNRLI
Sbjct: 215 RNLDIERPSYENLNRLI 231



 Score = 85.0 bits (201), Expect = 1e-17
 Identities = 36/53 (67%), Positives = 40/53 (75%)
 Frame = +1

Query: 145 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFF 303
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFF
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFF 53


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  177 bits (431), Expect = 2e-45
 Identities = 89/177 (50%), Positives = 110/177 (62%)
 Frame = +2

Query: 308 ETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEI 487
           ETG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE+
Sbjct: 59  ETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118

Query: 488 VDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALGSLPY*WSVSPLTTARSLNWSSPSTPR 667
           +D VL+RIR++AD C+GLQGFL+FHSFGGGT  G          +   +  N      P 
Sbjct: 119 IDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178

Query: 668 LRFPLXVVEPYNSIXXXXXXXXXXXXCFHGRQ*XHL*HLPPXXDIXRPT*TNLNRLI 838
            +    VVEPYNS+             F               DI RPT  NLNRLI
Sbjct: 179 PQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLI 235



 Score = 79.0 bits (186), Expect = 8e-16
 Identities = 40/58 (68%), Positives = 42/58 (72%), Gaps = 5/58 (8%)
 Frame = +1

Query: 145 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFF 303
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFF
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFF 57


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  107 bits (258), Expect = 1e-24
 Identities = 48/94 (51%), Positives = 63/94 (67%)
 Frame = +2

Query: 308 ETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEI 487
           E   GK+VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+
Sbjct: 53  EAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAEL 112

Query: 488 VDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALG 589
            D VLD +R+ A+ C  LQGF + HS GGGT  G
Sbjct: 113 ADAVLDVVRREAEACDALQGFQLTHSLGGGTGSG 146



 Score = 34.7 bits (76), Expect = 0.017
 Identities = 14/34 (41%), Positives = 18/34 (52%)
 Frame = +1

Query: 145 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDG 246
           MRE + +  GQ G Q+G A W     EHG+   G
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAG 34



 Score = 27.9 bits (59), Expect = 1.9
 Identities = 11/33 (33%), Positives = 20/33 (60%)
 Frame = +1

Query: 580 GSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVS 678
           GSG  +LL+ ++  +Y  +    F++ PAP+ S
Sbjct: 144 GSGMGTLLLSKIREEYPDRMMATFSVAPAPKSS 176


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 74.1 bits (174), Expect = 2e-14
 Identities = 35/91 (38%), Positives = 56/91 (61%), Gaps = 2/91 (2%)
 Frame = +2

Query: 323 KHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDL 496
           +++PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + 
Sbjct: 59  RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFED 117

Query: 497 VLDRIRKLADQCTGLQGFLIFHSFGGGTALG 589
           ++D I + AD    L+GF + HS  GGT  G
Sbjct: 118 IMDMIDREADGSDSLEGFSLLHSIAGGTGSG 148



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 21/42 (50%), Positives = 27/42 (64%)
 Frame = +1

Query: 148 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIG 273
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T G
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEG 44



 Score = 28.3 bits (60), Expect = 1.4
 Identities = 13/31 (41%), Positives = 19/31 (61%)
 Frame = +1

Query: 580 GSGFTSLLMERLSVDYGKKSKLEFAIYPAPQ 672
           GSG  S L+ERL+  Y KK    ++++P  Q
Sbjct: 146 GSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176


>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 830

 Score = 28.3 bits (60), Expect = 1.4
 Identities = 14/46 (30%), Positives = 26/46 (56%)
 Frame = -3

Query: 634 SCRSQRRDAPSIRK*TQSRTSTEGVEDQESLQTGTLVSELADSVQN 497
           S +       SIR  ++SR+S+ G + Q S+Q+GT    ++D  ++
Sbjct: 98  SSQKSSNSRKSIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,416,997
Number of Sequences: 5004
Number of extensions: 70471
Number of successful extensions: 218
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 211
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 414453330
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)