Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP08_F_A16
(882 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 235 5e-63
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 9e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 3e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 124 2e-29
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 1.5
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.7
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.5
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.7
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 6.2
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 8.2
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 235 bits (576), Expect = 5e-63
Identities = 101/132 (76%), Positives = 117/132 (88%)
Frame = +2
Query: 137 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 316
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 317 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 496
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 497 RKEAESCDCLQG 532
R+EAE+CD LQG
Sbjct: 121 RREAEACDALQG 132
Score = 88.2 bits (209), Expect = 1e-18
Identities = 40/59 (67%), Positives = 47/59 (79%)
Frame = +1
Query: 622 RIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCVDNEALYDICFRTLNYPHP 798
R+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+C+DNEAL I TL P
Sbjct: 162 RMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSP 220
Score = 44.0 bits (99), Expect = 3e-05
Identities = 34/118 (28%), Positives = 52/118 (44%), Gaps = 3/118 (2%)
Frame = +3
Query: 522 ASRGFQXXXXXXXXXXXXXXXXXXXKIREEYPXQNNEHIFSCTIT*SVGHSSRTVQRH-- 695
A +GFQ KIREEYP + + + ++ + S V+ +
Sbjct: 129 ALQGFQLTHSLGGGTGSGMGTLLLSKIREEYP---DRMMATFSVAPAPKSSDTVVEPYNA 185
Query: 696 TFSSSIS*KHRRNLLCRQRGSLRYLLPHA-KLSTPTYGDLNHLVSXTMSGVTTCLRXP 866
T S ++ C +L + + K+ +P+Y DLNHLVS M+GVTT R P
Sbjct: 186 TLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFP 243
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 135 bits (326), Expect = 9e-33
Identities = 62/134 (46%), Positives = 85/134 (63%), Gaps = 2/134 (1%)
Frame = +2
Query: 137 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 310
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 311 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 490
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 491 VVRKEAESCDCLQG 532
+R+ A++C LQG
Sbjct: 121 KIRRIADNCSGLQG 134
Score = 63.3 bits (147), Expect = 4e-11
Identities = 27/54 (50%), Positives = 38/54 (70%)
Frame = +1
Query: 637 YSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCVDNEALYDICFRTLNYPHP 798
+SV P+P+VS +VVEPYN+ L+ H ++ D T+ VDNE+ YDIC R L+ P
Sbjct: 169 FSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERP 222
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 130 bits (314), Expect = 3e-31
Identities = 59/138 (42%), Positives = 86/138 (62%), Gaps = 6/138 (4%)
Frame = +2
Query: 137 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 298
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 299 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 478
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 479 SVLDVVRKEAESCDCLQG 532
SVL+ +R+ A++C LQG
Sbjct: 121 SVLERIRRMADNCSGLQG 138
Score = 68.5 bits (160), Expect = 1e-12
Identities = 29/54 (53%), Positives = 40/54 (74%)
Frame = +1
Query: 637 YSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCVDNEALYDICFRTLNYPHP 798
+SV P+P+VS +VVEPYN+ L+ H ++N+D T+ VDNEA YDIC R L+ P
Sbjct: 173 FSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERP 226
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 124 bits (299), Expect = 2e-29
Identities = 52/133 (39%), Positives = 90/133 (67%), Gaps = 2/133 (1%)
Frame = +2
Query: 140 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 319
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 320 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 493
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 494 VRKEAESCDCLQG 532
+ +EA+ D L+G
Sbjct: 122 IDREADGSDSLEG 134
Score = 49.2 bits (112), Expect = 8e-07
Identities = 26/60 (43%), Positives = 36/60 (60%), Gaps = 1/60 (1%)
Frame = +1
Query: 622 RIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCVDNEALYDICFRTLNYPHP 798
+I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I L+ +P
Sbjct: 164 KIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNP 223
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/24 (54%), Positives = 14/24 (58%)
Frame = +3
Query: 795 PTYGDLNHLVSXTMSGVTTCLRXP 866
PT+ N LVS MS TT LR P
Sbjct: 223 PTFHQQNQLVSTVMSASTTTLRYP 246
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 28.3 bits (60), Expect = 1.5
Identities = 18/44 (40%), Positives = 24/44 (54%)
Frame = +3
Query: 666 GHSSRTVQRHTFSSSIS*KHRRNLLCRQRGSLRYLLPHAKLSTP 797
G+ RT +R F S ++R +LL R SLR L P A +TP
Sbjct: 70 GNRDRTTERSAFRS----RYRGSLLNRNSPSLRSLSPPATPATP 109
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +2
Query: 440 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 538
A+GH G ELV + D +RK++E+ L+ P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.5
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 176 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 277
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 371 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 472
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 6.2
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -1
Query: 513 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 376
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 8.2
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 392 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 523
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,235,211
Number of Sequences: 5004
Number of extensions: 63389
Number of successful extensions: 196
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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