Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP07_F_P08
(885 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 47 3e-06
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 44 2e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.010
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 32 0.12
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 32 0.12
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 30 0.50
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 29 0.67
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.88
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 1.5
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 2.7
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 2.7
SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 27 3.6
SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 27 4.7
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 27 4.7
SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit L37|... 27 4.7
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 26 8.2
SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 26 8.2
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 47.2 bits (107), Expect = 3e-06
Identities = 30/107 (28%), Positives = 53/107 (49%), Gaps = 1/107 (0%)
Frame = +3
Query: 405 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSES 584
D DG+ + E ++ G +E +++ ++ D D +G+I EFL + M ++
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDT 81
Query: 585 -RRNIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQTRYKSGEETEDV 722
V +AFK DK G+G IT++++ V + S S EE D+
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHV--LTSLGERLSQEEVADM 126
Score = 37.1 bits (82), Expect = 0.003
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +3
Query: 393 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 551
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 44.4 bits (100), Expect = 2e-05
Identities = 33/144 (22%), Positives = 67/144 (46%), Gaps = 6/144 (4%)
Frame = +3
Query: 276 EELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGI 455
E + ++ + + +++ L + AS L R+F +D+DG ++ +EF+ +
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDEDGGGDVDFQEFINSL 82
Query: 456 KETGLELNKSEAEEL-FSQFDTDSSGSISLDEFLIKIRPPMSESRR-----NIVEQAFKK 617
+ NK E + F +D D G IS E + ++ + + R IV++ +
Sbjct: 83 SVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIME 142
Query: 618 LDKTGDGAITIDDIKGVYSVDSQT 689
+DK DG I+ ++ K + S + T
Sbjct: 143 VDKDRDGKISFEEFKDIVSGSNVT 166
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.5 bits (78), Expect = 0.010
Identities = 18/97 (18%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
Frame = +3
Query: 381 LGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL-I 557
+ F+ D D ++ E ++ G KSE ++ FD G + +++F+ +
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 558 KIRPPMSESRRNIVEQAFKKLDKTGDGAITIDDIKGV 668
+ +++AF+ D G I++ +++ V
Sbjct: 99 MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRV 135
Score = 33.1 bits (72), Expect = 0.054
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +3
Query: 387 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 554
R F DDD + ++ KE ++ E E + +FD D G I+ EF+
Sbjct: 114 RAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
Score = 32.7 bits (71), Expect = 0.071
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = +3
Query: 471 ELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 650
E + + E F FD+D +I E +R + ++ V + + DKTG G + +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 651 DD 656
+D
Sbjct: 93 ED 94
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 31.9 bits (69), Expect = 0.12
Identities = 22/96 (22%), Positives = 40/96 (41%), Gaps = 2/96 (2%)
Frame = +3
Query: 378 GLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLI 557
G+ ++FR + S +LN+ EF + GL + E LF + G ++ + F
Sbjct: 488 GITKVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG-VTYERFTE 546
Query: 558 KIRPPMSESRRNIVEQAFKKLDKTGDGA--ITIDDI 659
+ + + R+ Q DG +T DD+
Sbjct: 547 IVMEELED--RDSARQVLYAFCDVADGKSYVTSDDL 580
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 31.9 bits (69), Expect = 0.12
Identities = 28/104 (26%), Positives = 46/104 (44%), Gaps = 6/104 (5%)
Frame = +3
Query: 426 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVE 602
LNK EF K+ + S AE +F+ FD D +G I EF+ + + +
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLI 102
Query: 603 QAFKKLDKTGDGAITIDD----IKGVYS-VDSQTRYKSGEETED 719
AF+ D +G I+ D+ + +Y V S + E+T +
Sbjct: 103 WAFQLYDLDNNGLISYDEMLRIVDAIYKMVGSMVKLPEDEDTPE 146
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 29.9 bits (64), Expect = 0.50
Identities = 18/74 (24%), Positives = 31/74 (41%)
Frame = +3
Query: 438 EFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKK 617
E+ G+ + + S LF +FD +GS+SL + + I + F+
Sbjct: 564 EWAKGLDAAAINNSSSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISFIFEL 623
Query: 618 LDKTGDGAITIDDI 659
D GDG + D+
Sbjct: 624 YDFNGDGFMDKPDV 637
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 29.5 bits (63), Expect = 0.67
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +1
Query: 481 KVKPKNSSVNSTQTVVAQSVLMNSLLKSVLPCRNRVVT-L*NKHSRSLTRLVTVQSQL 651
K+ KN+ S + + ++ L L L + +++ L NK S TRL +QSQL
Sbjct: 765 KLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQL 822
>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 29.1 bits (62), Expect = 0.88
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3
Query: 636 GAITIDDIKGVYSVDSQTRYKSGEETEDVY 725
G I+I D GVYS + T YKS E+ E ++
Sbjct: 126 GVISIHDSTGVYSQITTTPYKSLEDYEQLF 155
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 28.3 bits (60), Expect = 1.5
Identities = 23/80 (28%), Positives = 39/80 (48%), Gaps = 2/80 (2%)
Frame = +3
Query: 468 LELNKSEA--EELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGA 641
L LN S EE F + D D SG +S +EF + + ++R IV+ FK+ +G
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEFQHFV--SLLKTRSEIVD-IFKEY-TSGSDK 381
Query: 642 ITIDDIKGVYSVDSQTRYKS 701
++++ + S + R S
Sbjct: 382 MSLEQFRHFLSTSQKARLDS 401
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 27.5 bits (58), Expect = 2.7
Identities = 22/71 (30%), Positives = 32/71 (45%)
Frame = +3
Query: 441 FLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKL 620
F+ +E+ LE E FSQ +S + SL+ I P SRR IVEQ ++
Sbjct: 680 FILPNEESLLEKYWINYNESFSQLSRESLFT-SLESPFTDIESPTIVSRRKIVEQRKLRM 738
Query: 621 DKTGDGAITID 653
+K +D
Sbjct: 739 EKESFQETNVD 749
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 27.5 bits (58), Expect = 2.7
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +3
Query: 573 MSESRRNIVEQAFKKLDKTGDGAITIDDIK 662
++ S+ +++AF LDK GDG I +D+K
Sbjct: 42 LTSSQIQELKEAFALLDKDGDGNIGREDVK 71
>SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 488
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +3
Query: 213 KPKTSSNIAWHRPMSAGSVQEEELMQK 293
KPK S ++W+ + GS +E EL+ K
Sbjct: 56 KPKKDSLLSWNILLKKGSYKENELLAK 82
>SPCC622.12c |||NADP-specific glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 451
Score = 26.6 bits (56), Expect = 4.7
Identities = 12/41 (29%), Positives = 18/41 (43%), Gaps = 2/41 (4%)
Frame = +2
Query: 173 GCNVILNSKNVFRKTE--NIFQHCVAQTDVCWFRPGGRADA 289
GC + N+ E +F+ A + CW PG A+A
Sbjct: 331 GCRYVAEGSNMGSSAEAVEVFEKSRASGEGCWLAPGKAANA 371
>SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1369
Score = 26.6 bits (56), Expect = 4.7
Identities = 35/149 (23%), Positives = 61/149 (40%), Gaps = 11/149 (7%)
Frame = +3
Query: 321 DPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEEL 500
D E+ + S +S + L I + GS+L E F I G+ + AE
Sbjct: 793 DNYEQAESIISSLSSSALSELSYISESSMNIGSRL--DERF---IDANGVAIRDFSAELT 847
Query: 501 FSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKK--------LDKTGDGAITIDD 656
+ + +S G +S+D FL K++ + + + F+K DK DD
Sbjct: 848 YLTPE-NSKGKLSIDHFLNKVQSRWHDEEHHYYKTGFRKRVYKYLKIKDKKSKDVDPDDD 906
Query: 657 IKGVYSVDSQT--RYKSGEETE-DVYXXV 734
+ +++ T RYKS T ++Y +
Sbjct: 907 LVNQLPLNAYTKPRYKSAASTRLNIYQRI 935
>SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit
L37|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 26.6 bits (56), Expect = 4.7
Identities = 16/48 (33%), Positives = 23/48 (47%)
Frame = +1
Query: 430 TKKNSFMASRKQGWNLIKVKPKNSSVNSTQTVVAQSVLMNSLLKSVLP 573
+ +NS + K+ + +V PK NS T AQ V + SVLP
Sbjct: 32 SSRNSSSSLVKRSYVSSRVSPKKPQHNSDATSSAQKVANKTHTSSVLP 79
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 25.8 bits (54), Expect = 8.2
Identities = 10/34 (29%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -1
Query: 450 HKGIL-LCSVVYFHHHPCDGKFFQVPRSLKHLGT 352
H+G++ +C++VY KF + P++++ L T
Sbjct: 686 HRGLVCICNIVYSKDQEIFNKFIKTPKAVETLRT 719
>SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 981
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/47 (27%), Positives = 26/47 (55%)
Frame = -2
Query: 725 IYIFSFFAALISGLRIDRVNTFNVINCDCTVTSLVKLLECLFYNVTT 585
I+ FF A+ S ++ +N+ N +CT S+ +++ YNV++
Sbjct: 572 IHSVRFFHAIQSHFHLEGPIRYNM-NSNCTPNSIASIIQKKSYNVSS 617
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,585,697
Number of Sequences: 5004
Number of extensions: 50113
Number of successful extensions: 174
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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