Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP07_F_M23
(887 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 39 0.001
SPAC27E2.02 |||IMPACT homolog|Schizosaccharomyces pombe|chr 1|||... 37 0.004
SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosacc... 28 1.5
SPAC2G11.04 |||RNA-binding protein, G-patch type |Schizosaccharo... 27 3.6
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 27 3.6
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 26 6.2
SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos... 26 6.2
SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6 |Schi... 26 6.2
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 38.7 bits (86), Expect = 0.001
Identities = 18/62 (29%), Positives = 36/62 (58%)
Frame = +2
Query: 353 EVLLYVTLPEDYPSISPPKFELSAPWMDRQTKTNLHKTLHEIYLDNVGETVIFQWVEMIR 532
++++ LPE YP SPP F L + W+ + K L +L +++ + + + V+F +E +R
Sbjct: 71 DLVMEFFLPEAYPFNSPPTFFLKSSWLPLKQKRVLTSSLIKLW-NEIHDCVLFDAIEHVR 129
Query: 533 EV 538
+
Sbjct: 130 SI 131
>SPAC27E2.02 |||IMPACT homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 280
Score = 36.7 bits (81), Expect = 0.004
Identities = 33/106 (31%), Positives = 57/106 (53%), Gaps = 2/106 (1%)
Frame = +2
Query: 230 VDNLSRQAEEIEALKSIYTEELTIDSEITRSYTIRIEENKKEVLLYVTLPEDYPSISPPK 409
++N +E+ AL+SIY L SE + +YT+ I ++ V L + P DYP+ +P
Sbjct: 1 MENNEEFQDELLALESIYPSCLLPISEQSFTYTLSIPDS--SVRLNIQFPLDYPNSAPT- 57
Query: 410 FELSAPWMDRQTKTNLHKTLHEIYLDNV--GETVIFQWVEMIREVL 541
L A +D KTL E L +V G+ IF ++++++E++
Sbjct: 58 -VLDAYGID--------KTLAEDVLLSVATGDVCIFSYMDLLKELV 94
Score = 29.1 bits (62), Expect = 0.88
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +3
Query: 648 EIIADRKSIFXGHAAEVHSINDVKAVL 728
E I DRKS F HA V+S +V+ L
Sbjct: 148 EPITDRKSTFMAHATRVYSTEEVREAL 174
>SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase
Ubp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1141
Score = 28.3 bits (60), Expect = 1.5
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = -1
Query: 470 ARFCEDSFWFVCPSTVQTIRISVEKSTGNLLVMSRKE 360
A F E+ FW VC T + + + V + G L+ +E
Sbjct: 53 AVFDENDFWCVCQKTRKHLHVKVRRDRGKPLIEEPEE 89
>SPAC2G11.04 |||RNA-binding protein, G-patch type
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 301
Score = 27.1 bits (57), Expect = 3.6
Identities = 19/83 (22%), Positives = 36/83 (43%), Gaps = 5/83 (6%)
Frame = +2
Query: 335 IEENKKEVLLYVTLPEDYPSI----SPPKFELSAP-WMDRQTKTNLHKTLHEIYLDNVGE 499
I++N E + +V D S +P F++ + + N ++ DN+G+
Sbjct: 87 IQKNANENIPHVQCRNDSQSTVTTRNPTPFKIEVGIYPSKPNNLNKEPPASTVHSDNLGD 146
Query: 500 TVIFQWVEMIREVLQTVCKVEKK 568
V +WVE+ + T V K+
Sbjct: 147 AVEHEWVELYDPLFPTSYSVFKE 169
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 27.1 bits (57), Expect = 3.6
Identities = 32/112 (28%), Positives = 52/112 (46%), Gaps = 14/112 (12%)
Frame = +2
Query: 248 QAEEIEALKSIYT---EELTIDS--EITRS--YTIRI---EENKKEVL---LYVTLPEDY 388
Q EIEALK+I+ EEL + + +T Y I + N K + L + L Y
Sbjct: 14 QENEIEALKAIFMDDFEELKVRNAWNVTNGHVYCIHLCSRSANSKSIAKLDLCIELGRSY 73
Query: 389 PSISPP-KFELSAPWMDRQTKTNLHKTLHEIYLDNVGETVIFQWVEMIREVL 541
P + P K + ++ Q + L K L D +GE +IF+ ++++ L
Sbjct: 74 PYVKPVIKLQNGENVLNSQIRFLLDK-LDTKAKDLLGEEMIFELASIVQDYL 124
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 26.2 bits (55), Expect = 6.2
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = +2
Query: 251 AEEIEALKSIYTEELTIDSEITRSYTIRIEENKKEVLLYVTLPEDYPSISP 403
A ++EAL ++ ++L +DS + R+ E V+L+ T+ + PS P
Sbjct: 1272 AHQVEALMELFEQKLNVDS-LPTDANDRLRE--ATVVLFGTVAQHLPSNDP 1319
>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 26.2 bits (55), Expect = 6.2
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -3
Query: 630 GQFISIVVRSKLSTSGSVTSIFFSTLHTV 544
G +I ++ L T G +TS FFS L +V
Sbjct: 105 GLLQNINTKNPLGTGGKITSAFFSLLQSV 133
>SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 624
Score = 26.2 bits (55), Expect = 6.2
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +3
Query: 429 GWTDKPKRIFTKPCTKYT 482
GW P+ +FT+P T++T
Sbjct: 430 GWLSLPRSLFTQPFTEFT 447
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,959,739
Number of Sequences: 5004
Number of extensions: 53995
Number of successful extensions: 170
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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