Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP07_F_E14
         (868 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   220   2e-58
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   217   2e-57
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   125   7e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    90   3e-19
SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pomb...    26   8.0  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  220 bits (538), Expect = 2e-58
 Identities = 100/156 (64%), Positives = 124/156 (79%)
 Frame = +3

Query: 264 PQTRPSGVETILSTLSSVETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGK 443
           P+T     +   ST  S ETG GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGK
Sbjct: 38  PETASQNSDGGFSTFFS-ETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGK 96

Query: 444 EDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMER 623
           EDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH            LL+ER
Sbjct: 97  EDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLER 156

Query: 624 LSVDYGKKSKLEFAIYPAPQVSTXVVEPYNSILTTH 731
           L+++Y KKSKL+F++YPAPQVST VVEPYNS+LTTH
Sbjct: 157 LAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTH 192



 Score = 75.4 bits (177), Expect = 1e-14
 Identities = 32/40 (80%), Positives = 36/40 (90%)
 Frame = +1

Query: 748 SDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSS 867
           +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSS
Sbjct: 198 ADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSS 237



 Score = 52.0 bits (119), Expect = 1e-07
 Identities = 21/24 (87%), Positives = 22/24 (91%)
 Frame = +1

Query: 154 MRECISVHVGQAGVQIGNACWELY 225
           MRE IS+HVGQAG QIGNACWELY
Sbjct: 1   MREIISIHVGQAGTQIGNACWELY 24



 Score = 38.7 bits (86), Expect = 0.001
 Identities = 16/30 (53%), Positives = 19/30 (63%)
 Frame = +2

Query: 227 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFS 316
           CLEHGIQP+G M  +      D  F+TFFS
Sbjct: 25  CLEHGIQPNGYMNPETASQNSDGGFSTFFS 54


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  217 bits (529), Expect = 2e-57
 Identities = 92/138 (66%), Positives = 117/138 (84%)
 Frame = +3

Query: 318 ETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEI 497
           ETG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE+
Sbjct: 59  ETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118

Query: 498 VDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 677
           +D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPA
Sbjct: 119 IDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178

Query: 678 PQVSTXVVEPYNSILTTH 731
           PQVST VVEPYNS+LTTH
Sbjct: 179 PQVSTSVVEPYNSVLTTH 196



 Score = 80.6 bits (190), Expect = 3e-16
 Identities = 35/42 (83%), Positives = 38/42 (90%)
 Frame = +1

Query: 742 EHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSS 867
           ++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSS
Sbjct: 200 DNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSS 241



 Score = 54.4 bits (125), Expect = 2e-08
 Identities = 27/44 (61%), Positives = 31/44 (70%), Gaps = 1/44 (2%)
 Frame = +1

Query: 154 MRECISVHVGQAGVQIGNACWELY-LPGARHPA*WPDAHRQDHR 282
           MRE ISVHVGQAGVQIGNACWELY L     P  +P  + + H+
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHK 44


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  125 bits (302), Expect = 7e-30
 Identities = 58/138 (42%), Positives = 83/138 (60%)
 Frame = +3

Query: 318 ETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEI 497
           E   GK+VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+
Sbjct: 53  EAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAEL 112

Query: 498 VDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 677
            D VLD +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PA
Sbjct: 113 ADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPA 172

Query: 678 PQVSTXVVEPYNSILTTH 731
           P+ S  VVEPYN+ L+ H
Sbjct: 173 PKSSDTVVEPYNATLSMH 190



 Score = 39.5 bits (88), Expect = 6e-04
 Identities = 17/44 (38%), Positives = 27/44 (61%)
 Frame = +1

Query: 733 QXXEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVS 864
           Q  E+SD  F +DNEA+  I    L I+ P+Y +LN L+  +++
Sbjct: 191 QLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMA 234



 Score = 27.5 bits (58), Expect = 2.6
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = +1

Query: 154 MRECISVHVGQAGVQIGNACW 216
           MRE + +  GQ G Q+G A W
Sbjct: 1   MREIVHIQAGQCGNQVGAAFW 21


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 90.2 bits (214), Expect = 3e-19
 Identities = 48/133 (36%), Positives = 77/133 (57%), Gaps = 3/133 (2%)
 Frame = +3

Query: 333 KHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDL 506
           +++PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + 
Sbjct: 59  RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFED 117

Query: 507 VLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ- 683
           ++D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q 
Sbjct: 118 IMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQS 177

Query: 684 VSTXVVEPYNSIL 722
           VS  VV+PYNS+L
Sbjct: 178 VSDVVVQPYNSLL 190



 Score = 28.3 bits (60), Expect = 1.5
 Identities = 11/41 (26%), Positives = 23/41 (56%)
 Frame = +1

Query: 745 HSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSS 867
           ++D   ++DN A+  I    L  + PT+   N+L+  ++S+
Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSA 238



 Score = 27.5 bits (58), Expect = 2.6
 Identities = 11/19 (57%), Positives = 13/19 (68%)
 Frame = +2

Query: 227 CLEHGIQPDGQMPTDKTIG 283
           CLEHGI PDG + +  T G
Sbjct: 26  CLEHGIGPDGTLESFATEG 44


>SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 990

 Score = 25.8 bits (54), Expect = 8.0
 Identities = 11/40 (27%), Positives = 17/40 (42%)
 Frame = +1

Query: 199 IGNACWELYLPGARHPA*WPDAHRQDHRGWRRFFQHFLQS 318
           +  A W++ LP       WP + R     +RR     L+S
Sbjct: 412 VEKAWWKMLLPSPEQWNSWPSSSRDSFNSYRRDLGDLLES 451


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,494,265
Number of Sequences: 5004
Number of extensions: 69930
Number of successful extensions: 208
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)