Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP07_F_B22
         (891 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23C4.15 |rpb5||DNA-directed RNA polymerase I, II and III sub...   136   3e-33
SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb...    27   3.6  
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca...    27   4.7  
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch...    27   4.7  
SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc...    26   6.3  
SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo...    26   6.3  
SPBC3B9.01 |||Hsp70 nucleotide exchange factor |Schizosaccharomy...    26   8.3  

>SPAC23C4.15 |rpb5||DNA-directed RNA polymerase I, II and III
           subunit Rpb5 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 210

 Score =  136 bits (330), Expect = 3e-33
 Identities = 73/182 (40%), Positives = 107/182 (58%), Gaps = 1/182 (0%)
 Frame = +2

Query: 116 DDAETYKLWRIRKTVMQLCHDRGYLVTQDELDQTLEQFKEQF-GDKPSEKRPARSDLIVL 292
           ++    +++R  KT  QL HDRGY V+Q ELD TL+QFK    G   +  R   S     
Sbjct: 4   EEKNIVRVFRAWKTAHQLVHDRGYGVSQAELDLTLDQFKAMHCGMGRNLDRTTLS--FYA 61

Query: 293 VAHNDDPTDQMFVFFPDEAKIGIKTIKTYCTRMQEENIHRAIVVVQAGMSPSAKQSLVDM 472
              ND     +++ F  E  +GIK ++T+   + + N    I++    M+PSA + +  +
Sbjct: 62  KPSNDSNKGTIYIEFAKEPSVGIKEMRTFVHTLGDHNHKTGILIYANSMTPSAAKIIATV 121

Query: 473 APKYILEQFLESELLINITEHELVPEHIVLTPDEKQELLARYKLKENMLMRIQAGDPVGE 652
             ++ +E F ES+L++NIT HELVP+HI+L+PDEK+ELL RYKL+E  L RIQ  DPV  
Sbjct: 122 TGQFTIETFQESDLIVNITHHELVPKHILLSPDEKKELLDRYKLRETQLPRIQLADPVAR 181

Query: 653 IL 658
            L
Sbjct: 182 YL 183



 Score = 51.2 bits (117), Expect = 2e-07
 Identities = 22/30 (73%), Positives = 27/30 (90%)
 Frame = +3

Query: 645 LARYFGLKRGQVVKIIRSSETAGRYISYRL 734
           +ARY GLKRG+VVKI+R SET+GRY SYR+
Sbjct: 179 VARYLGLKRGEVVKIVRRSETSGRYNSYRI 208


>SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 401

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
 Frame = +2

Query: 389 MQEENIHRAIV--VVQAGMSP--SAKQSLVDMAPKYILEQFLE 505
           + + N+HR I+    + GM    SAKQ L D+ P  I+  +LE
Sbjct: 78  VDKSNLHRQIIHSTSKQGMHKAISAKQFLEDLNPNVIINTYLE 120


>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
            synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 2410

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 17/59 (28%), Positives = 33/59 (55%), Gaps = 2/59 (3%)
 Frame = +2

Query: 470  MAPKYILEQFLESELLINITEHE-LVPEHIVLTPDEKQELLARYKLKENMLMR-IQAGD 640
            +AP Y+L   +   L ++  E + L P   + TP+EK+E+ A Y + + +  + +Q G+
Sbjct: 1285 LAPCYLLPDIIPCALSLHNAEFQGLWP---LRTPEEKEEVCAVYNISQRVCTKYVQFGN 1340


>SPCC162.08c |nup211||nuclear pore complex associated
           protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1837

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 16/81 (19%), Positives = 36/81 (44%), Gaps = 9/81 (11%)
 Frame = +2

Query: 194 TQDELDQTLEQFKEQFGDKPSEKRPARSDLIVL---------VAHNDDPTDQMFVFFPDE 346
           ++DELD++ +  +EQ   K  E +   S L +          +  N    + +     D+
Sbjct: 738 SKDELDESYKSLQEQLASKKIEVQNVSSQLSICNSQLEQSNHIVDNLKSENLLLTSVKDK 797

Query: 347 AKIGIKTIKTYCTRMQEENIH 409
            K  +  +++  + +Q++N H
Sbjct: 798 LKADLSNLESKLSSLQQDNFH 818


>SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1085

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
 Frame = +2

Query: 206  LDQTLEQFKEQFGDKPSEKRPARSDLI-VLVAHNDDPTDQM 325
            LD+ L++ KE   D   EK     DL+  LV +ND+  D +
Sbjct: 914  LDRLLQKVKEHSEDNTKEKHQQLLDLLESLVGNNDNLIDSI 954


>SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1616

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 15/73 (20%), Positives = 38/73 (52%), Gaps = 4/73 (5%)
 Frame = -3

Query: 427  YNNNSPMNILFLHSCTICLYSFYTN-FSFIRKENKHL---IRWIIIMSH*HNQITSCWSF 260
            +N +  +  +F  S   C Y+   + ++F   ++K +   + WI+++ H  +   + W+ 
Sbjct: 982  WNVSDTLKNMFSTSDFSCAYNSVEDLYAFTSMKSKEILPEVLWIMLLVHLADLCENSWAS 1041

Query: 259  LTWFIAKLLFKLF 221
            +    A++LF++F
Sbjct: 1042 VRNGAAQILFRIF 1054


>SPBC3B9.01 |||Hsp70 nucleotide exchange factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 287

 Score = 25.8 bits (54), Expect = 8.3
 Identities = 14/38 (36%), Positives = 19/38 (50%), Gaps = 1/38 (2%)
 Frame = -1

Query: 189 KYPRSWHNCITVLRIRQSL*VSASSSMFYNLL-KTDKS 79
           K P SW   I +L ++ S+        FY LL + DKS
Sbjct: 168 KIPNSWEMLIEILELKHSVMTKRVIFFFYALLIQEDKS 205


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,327,396
Number of Sequences: 5004
Number of extensions: 70292
Number of successful extensions: 185
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)