Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP06_F_J21
(944 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 136 6e-33
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 72 1e-13
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 65 1e-11
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 56 7e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 54 4e-08
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 41 3e-04
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 34 0.034
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 29 1.3
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 28 1.7
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 27 5.1
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 6.7
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 136 bits (328), Expect = 6e-33
Identities = 68/113 (60%), Positives = 83/113 (73%)
Frame = +1
Query: 148 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVNADGNGTI 327
LT+EQIAEF+EAFSLFD+D DG IT+ ELG VMRSLGQ+PT AELQDMINEV+ADGNGTI
Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65
Query: 328 DFSRVLDNDGAQDEGHG*RGXNPARPSRVFDKDGNGFISAAELXHVMTNLGXK 486
DF+ L + + +VFDKDGNG+I+ EL HV+T+LG +
Sbjct: 66 DFTEFLTMMARKMKDTD-NEEEVREAFKVFDKDGNGYITVEELTHVLTSLGER 117
Score = 64.5 bits (150), Expect = 2e-11
Identities = 34/75 (45%), Positives = 50/75 (66%), Gaps = 3/75 (4%)
Frame = +1
Query: 136 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVNADG 315
M D EE++ +EAF +FDKDG+G IT +EL V+ SLG+ ++ E+ DMI E + DG
Sbjct: 78 MKDTDNEEEV---REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDG 134
Query: 316 NGTI---DFSRVLDN 351
+G I +FSRV+ +
Sbjct: 135 DGVINYEEFSRVISS 149
Score = 58.0 bits (134), Expect = 2e-09
Identities = 34/78 (43%), Positives = 38/78 (48%)
Frame = +2
Query: 332 FPEFLTMMARKMKDTDSEEXIPRGLXXXXXXXXXXXXXXXXXXXXXXXXXXKLXDEEVXX 511
F EFLTMMARKMKDTD+EE + R +L EEV
Sbjct: 67 FTEFLTMMARKMKDTDNEEEV-REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVAD 125
Query: 512 MIRXADIDGXGQVNYXEF 565
MIR AD DG G +NY EF
Sbjct: 126 MIREADTDGDGVINYEEF 143
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 71.7 bits (168), Expect = 1e-13
Identities = 44/112 (39%), Positives = 59/112 (52%)
Frame = +1
Query: 151 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVNADGNGTID 330
++EQ E KEAF L+D D DG I T +G+V+RSLG N T+AEL + NE+ D
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL-GDAIDEKK 62
Query: 331 FSRVLDNDGAQDEGHG*RGXNPARPSRVFDKDGNGFISAAELXHVMTNLGXK 486
F + N + E + RVFDKD +G+I A+ M LG K
Sbjct: 63 FMSFVSNKLRETESE----EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEK 110
Score = 47.2 bits (107), Expect = 3e-06
Identities = 23/69 (33%), Positives = 42/69 (60%), Gaps = 1/69 (1%)
Frame = +1
Query: 130 STMADQLTE-EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVN 306
S ++++L E E E+ +AF +FDKD G I T + M++LG+ ++ E+Q M+ E +
Sbjct: 65 SFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEAD 124
Query: 307 ADGNGTIDF 333
+G+ D+
Sbjct: 125 PTNSGSFDY 133
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 65.3 bits (152), Expect = 1e-11
Identities = 38/105 (36%), Positives = 59/105 (56%)
Frame = +1
Query: 172 FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVNADGNGTIDFSRVLDN 351
+K+AFSLFD+ G G I +G ++R+ GQNPT AE+ ++ + + A+ + F +VL+
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVD-MEQFLQVLNR 66
Query: 352 DGAQDEGHG*RGXNPARPSRVFDKDGNGFISAAELXHVMTNLGXK 486
D + +VFDKD G I EL +V+T+LG K
Sbjct: 67 PNGFDMPGD--PEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEK 109
Score = 37.1 bits (82), Expect = 0.004
Identities = 17/45 (37%), Positives = 26/45 (57%)
Frame = +1
Query: 169 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 303
EF + F +FDKD G I EL V+ SLG+ + E+ +++ V
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV 122
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 56.0 bits (129), Expect = 7e-09
Identities = 25/54 (46%), Positives = 35/54 (64%)
Frame = +1
Query: 145 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVN 306
QLT QI E KEAF+L DKDGDG I +++ T++ SL Q+ +E + M +N
Sbjct: 41 QLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
Score = 26.2 bits (55), Expect = 6.7
Identities = 12/46 (26%), Positives = 20/46 (43%)
Frame = +1
Query: 178 EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVNADG 315
EAFS FD G I + + S+G E++ ++ + G
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSYTSHG 166
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 53.6 bits (123), Expect = 4e-08
Identities = 37/114 (32%), Positives = 53/114 (46%), Gaps = 3/114 (2%)
Frame = +1
Query: 145 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVNADGNGT 324
++TEEQ + EAF LFD D D I EL MR+LG N ++E+ ++ + + G G
Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89
Query: 325 I---DFSRVLDNDGAQDEGHG*RGXNPARPSRVFDKDGNGFISAAELXHVMTNL 477
+ DF RV+ + + R +FD D G IS L V L
Sbjct: 90 LQMEDFVRVMTEKIVERDPL----EEIKRAFELFDDDETGKISLRNLRRVAKEL 139
Score = 26.2 bits (55), Expect = 6.7
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +2
Query: 488 LXDEEVXXMIRXADIDGXGQVNYXEF 565
+ D+E+ MI D+D G++N EF
Sbjct: 143 IDDQELEAMIEEFDLDQDGEINEQEF 168
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 40.7 bits (91), Expect = 3e-04
Identities = 28/106 (26%), Positives = 51/106 (48%)
Frame = +1
Query: 151 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVNADGNGTID 330
+ E+I ++ F D + G+I E ++ S+ NP + L +++E DG G +D
Sbjct: 19 SNEEIERIRKRFIKIDANQSGSIDRNEFLSIP-SVASNPLASRLFSVVDE---DGGGDVD 74
Query: 331 FSRVLDNDGAQDEGHG*RGXNPARPSRVFDKDGNGFISAAELXHVM 468
F + N + HG + +++D D +G+IS EL V+
Sbjct: 75 FQEFI-NSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVL 119
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 33.9 bits (74), Expect = 0.034
Identities = 19/53 (35%), Positives = 28/53 (52%)
Frame = +1
Query: 157 EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVNADG 315
E E +EAF LFD G I ++L LG+N T+ +LQ M++ +G
Sbjct: 9 EMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNG 61
>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1011
Score = 28.7 bits (61), Expect = 1.3
Identities = 15/57 (26%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
Frame = +1
Query: 142 DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLG--QNPTEAELQDMINEVN 306
D+ E A + +S+FD+D +G IT +E+ +G + A L+D+ + ++
Sbjct: 538 DETGEVDNATLEACYSIFDRDLNGDITCEEIELACVEIGKERKSISASLRDLNDSIS 594
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 28.3 bits (60), Expect = 1.7
Identities = 21/75 (28%), Positives = 31/75 (41%)
Frame = -1
Query: 464 TWXSSAAEMKPLPSLSKTREGLAGFXPRYPCPSSCAPSLSRTREKSIVPFPSAFTSLIMS 285
T SSA P + G + P P+S S S S++P S+FT+ S
Sbjct: 161 TIYSSATSSFPYSTDVSVSTGTSTDIVTLPPPASSTSSFSTITNTSMIPSSSSFTTTTGS 220
Query: 284 *SSASVGFCPSDLIT 240
+ F PS +I+
Sbjct: 221 PYYNTSSFLPSSVIS 235
>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
zf-fungal binuclear cluster type |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 977
Score = 26.6 bits (56), Expect = 5.1
Identities = 9/27 (33%), Positives = 15/27 (55%)
Frame = -2
Query: 286 LEVRLLWGSVLATSSRCPALWS*WCHR 206
LE++ WGS+ A + +W+ W R
Sbjct: 850 LEMKNYWGSISALCDKMSEIWADWVQR 876
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 26.2 bits (55), Expect = 6.7
Identities = 14/40 (35%), Positives = 17/40 (42%)
Frame = -1
Query: 428 PSLSKTREGLAGFXPRYPCPSSCAPSLSRTREKSIVPFPS 309
P + K+ G P P PSS PS+ VP PS
Sbjct: 1050 PPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPS 1089
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,354,932
Number of Sequences: 5004
Number of extensions: 36751
Number of successful extensions: 116
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 481321826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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