Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP06_F_J03
(906 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 132 7e-32
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 71 3e-13
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 69 1e-12
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 68 2e-12
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 60 3e-10
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 48 2e-06
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 44 2e-05
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 36 0.006
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 29 0.91
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 132 bits (319), Expect = 7e-32
Identities = 62/113 (54%), Positives = 84/113 (74%)
Frame = +1
Query: 235 GLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQRAR 414
G+TSV + G + A V +Q+KVPD+L+D +V H+F +T IGCVMTG IAD+R+QV RAR
Sbjct: 34 GITSVGVTGKNCACVISQKKVPDKLIDASTVKHVFPITKGIGCVMTGSIADARAQVSRAR 93
Query: 415 YEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAG 573
EAA ++YK G +P +L +R+A+I+QV TQ A MRPLG +M L+A DDE G
Sbjct: 94 SEAAEFEYKNGYPMPCDVLAKRMANIAQVSTQRAAMRPLGVAMTLVAVDDEIG 146
Score = 62.5 bits (145), Expect = 8e-11
Identities = 28/50 (56%), Positives = 33/50 (66%)
Frame = +3
Query: 150 GFDRHITIFSPEGRLYQVEYALKAINQGWVDICGITRYRCSCSGSSTQGP 299
GFDR IT+FSPEGRLYQVEYA KA N + G+T C+C S + P
Sbjct: 6 GFDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVP 55
Score = 37.5 bits (83), Expect = 0.003
Identities = 27/85 (31%), Positives = 35/85 (41%)
Frame = +2
Query: 572 GPCVYKTDPSGXXXXXXXXXXXXXXXXXXRLSRKETKKNARTSAPMMRFSLPSPGLXQVL 751
GP ++K DP+G K KK+ S L L
Sbjct: 146 GPSLFKLDPAGFYIGYKATSAGPKQTETINWLEKRFKKDGIPSNLTDTVETGIQALMSSL 205
Query: 752 SVDFKSSEIEIGVVSXDILRFRVLT 826
S DFKS+E++IGVV D FRVL+
Sbjct: 206 STDFKSTELQIGVVEDD-KPFRVLS 229
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 70.5 bits (165), Expect = 3e-13
Identities = 34/109 (31%), Positives = 58/109 (53%)
Frame = +1
Query: 241 TSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQRARYE 420
T + ++ D ++A ++ V +LL P + ++ IG TG I D + V+RAR E
Sbjct: 35 TCIGIKCEDGVILALEKVVTSKLLKPRVNNRIGSVDRHIGIATTGFIPDGQHIVKRARDE 94
Query: 421 AANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDE 567
A +W+ YGS IP ++ R+ + Q++T + +RP G + YD E
Sbjct: 95 ATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDSE 143
Score = 32.7 bits (71), Expect = 0.074
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +3
Query: 150 GFDRHITIFSPEGRLYQVEYALKAI 224
G+D + FSP+GRL+Q EYA KA+
Sbjct: 7 GYDLGL-FFSPDGRLFQAEYAYKAV 30
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 68.5 bits (160), Expect = 1e-12
Identities = 39/113 (34%), Positives = 60/113 (53%), Gaps = 2/113 (1%)
Frame = +1
Query: 229 RVGLTSVALRGTDAAVVAAQRK-VPDRLLDPVSVTHLFALTDRIGCV-MTGMIADSRSQV 402
R G T++ALRG + V+ +RK VP L VS A+ D C+ G+ AD+R +
Sbjct: 28 RRGTTAIALRGNECIVIGVERKNVPK--LQNVSNFQKIAMVDNHVCLAFAGLNADARILI 85
Query: 403 QRARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYD 561
+AR EA N + + + L R +A + Q YTQ+ +RP G S ++ +D
Sbjct: 86 DKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSGGVRPFGVSTLIAGFD 138
Score = 41.1 bits (92), Expect = 2e-04
Identities = 15/29 (51%), Positives = 24/29 (82%)
Frame = +3
Query: 147 AGFDRHITIFSPEGRLYQVEYALKAINQG 233
+G+DR +++FSP+GRL QVEY +A+ +G
Sbjct: 2 SGYDRALSVFSPDGRLLQVEYGQEAVRRG 30
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 68.1 bits (159), Expect = 2e-12
Identities = 40/158 (25%), Positives = 74/158 (46%), Gaps = 1/158 (0%)
Frame = +1
Query: 265 DAAVVAAQRKVPDRLLDPV-SVTHLFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYK 441
D V+AA++KV +LL+ S L+ + D + C + G+ AD+ + AR +
Sbjct: 41 DGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQT 100
Query: 442 YGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGALCVQNRPXXXXXXXX 621
+ E+P L RR+ D+ Q YTQ +RP G S + +D G Q+ P
Sbjct: 101 FNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPSGNYGSWQ 160
Query: 622 XXXXXXXXXXMETLISKRN*KKRSDLSTDDAIQLAISW 735
+++L+ + + + D++ D+A +A+ +
Sbjct: 161 ANSIGGNSTSVQSLMRQ---EYKDDINLDEASAMAVKF 195
Score = 48.0 bits (109), Expect = 2e-06
Identities = 22/32 (68%), Positives = 24/32 (75%)
Frame = +3
Query: 144 SAGFDRHITIFSPEGRLYQVEYALKAINQGWV 239
S +D TIFSPEGRLYQVEYAL+AIN V
Sbjct: 2 SRSYDSRTTIFSPEGRLYQVEYALEAINHAGV 33
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 60.5 bits (140), Expect = 3e-10
Identities = 33/129 (25%), Positives = 67/129 (51%), Gaps = 6/129 (4%)
Frame = +1
Query: 229 RVGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQR 408
++G T++ ++ DA V+ ++++ L++ SV LF + IGC ++G+ AD+R+ ++
Sbjct: 32 KLGSTAIGVKTKDAVVLGVEKRLTSPLMESHSVEKLFEIDSHIGCAISGLTADARTIIEH 91
Query: 409 ARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAE------MRPLGCSMMLIAYDDEA 570
AR + N ++ Y + + I D++ + + + RP G + +LIA DE
Sbjct: 92 ARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVA-LLIAGIDEH 150
Query: 571 GALCVQNRP 597
G + P
Sbjct: 151 GPQLYHSEP 159
Score = 41.9 bits (94), Expect = 1e-04
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +3
Query: 153 FDRHITIFSPEGRLYQVEYALKAINQG 233
+DR + FSPEGRL+QVEYA++AI G
Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIKLG 34
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 47.6 bits (108), Expect = 2e-06
Identities = 33/121 (27%), Positives = 57/121 (47%)
Frame = +1
Query: 235 GLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQRAR 414
G +V L AV+ A ++ + L L + D IG + G+ D+R +
Sbjct: 32 GSATVGLVSKTHAVLVALKRNAEELSSYQK--KLIRIDDHIGIAIAGLAPDARVLSNYMK 89
Query: 415 YEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGALCVQNR 594
EA + + + IPV L ++A+ +Q+ TQ RP G ++I Y DE+G ++ +
Sbjct: 90 QEALSSKTLFTRPIPVRRLMSKVAEKAQINTQEYGRRPYGVGFLVIGY-DESGPHLLEFQ 148
Query: 595 P 597
P
Sbjct: 149 P 149
Score = 39.9 bits (89), Expect = 5e-04
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +3
Query: 153 FDRHITIFSPEGRLYQVEYALKAINQG 233
+D T +SP+GRL+QVEYAL+AI QG
Sbjct: 6 YDGDATTWSPQGRLHQVEYALEAIKQG 32
>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 44.4 bits (100), Expect = 2e-05
Identities = 32/116 (27%), Positives = 54/116 (46%), Gaps = 1/116 (0%)
Frame = +1
Query: 229 RVGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVT-HLFALTDRIGCVMTGMIADSRSQVQ 405
R+G T ALR D ++AA + VT L LTD I C +G AD+++
Sbjct: 22 RMGTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVAD 81
Query: 406 RARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAG 573
+Y + ++ ++G + VH S++ QN M L +++ YD++ G
Sbjct: 82 LLKYYLSMYRIQFGHDPSVHTAATL---ASEMCYQNKNM--LSAGLIVAGYDEKTG 132
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 36.3 bits (80), Expect = 0.006
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +3
Query: 165 ITIFSPEGRLYQVEYALKAINQG 233
+T FSP G+L Q+EYAL A+N G
Sbjct: 9 LTTFSPNGKLVQIEYALNAVNAG 31
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 29.1 bits (62), Expect = 0.91
Identities = 15/62 (24%), Positives = 25/62 (40%)
Frame = +1
Query: 373 GMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLI 552
GM +R+ Q +E +W Y + V LCR + ++ N + + LI
Sbjct: 606 GMCPYTRAVSQHVEWENDSWMYVLNVSLQVAKLCRHVGNVFMELNTNKLANAINYLISLI 665
Query: 553 AY 558
Y
Sbjct: 666 LY 667
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,197,707
Number of Sequences: 5004
Number of extensions: 58351
Number of successful extensions: 142
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 458501510
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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