Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP05_F_I08
         (898 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   267   2e-72
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   266   2e-72
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   147   2e-36
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   100   6e-22

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  267 bits (654), Expect = 2e-72
 Identities = 115/168 (68%), Positives = 143/168 (85%)
 Frame = +1

Query: 310 ETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEI 489
           ETG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE+
Sbjct: 59  ETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118

Query: 490 VDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 669
           +D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPA
Sbjct: 119 IDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178

Query: 670 PXVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDIXRRNLDIERP 813
           P VST+VVEPYNS+LTTH TL++SDC FMVDNEA YDI RRNLDIERP
Sbjct: 179 PQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERP 226



 Score = 79.8 bits (188), Expect = 5e-16
 Identities = 41/59 (69%), Positives = 43/59 (72%), Gaps = 5/59 (8%)
 Frame = +3

Query: 147 MRERISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFS 308
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFS
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFS 58



 Score = 46.0 bits (104), Expect = 7e-06
 Identities = 21/29 (72%), Positives = 24/29 (82%)
 Frame = +3

Query: 810 PTYTNLNRLIGQIVSSIXXSLKFDGALNV 896
           PTY NLNRLI Q+VSSI  SL+F G+LNV
Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSLNV 254


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  266 bits (653), Expect = 2e-72
 Identities = 121/186 (65%), Positives = 149/186 (80%)
 Frame = +1

Query: 256 PQTRPSGVETILSTLSSVETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGK 435
           P+T     +   ST  S ETG GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGK
Sbjct: 38  PETASQNSDGGFSTFFS-ETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGK 96

Query: 436 EDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMER 615
           EDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH            LL+ER
Sbjct: 97  EDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLER 156

Query: 616 LSVDYGKKSKLEFAIYPAPXVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDIXRRN 795
           L+++Y KKSKL+F++YPAP VST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDI RRN
Sbjct: 157 LAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRN 216

Query: 796 LDIERP 813
           LDIERP
Sbjct: 217 LDIERP 222



 Score = 85.8 bits (203), Expect = 7e-18
 Identities = 37/54 (68%), Positives = 41/54 (75%)
 Frame = +3

Query: 147 MRERISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFS 308
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFS
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFS 54



 Score = 46.0 bits (104), Expect = 7e-06
 Identities = 20/29 (68%), Positives = 25/29 (86%)
 Frame = +3

Query: 810 PTYTNLNRLIGQIVSSIXXSLKFDGALNV 896
           P+Y NLNRLI Q+VSSI  SL+F+G+LNV
Sbjct: 222 PSYENLNRLIAQVVSSITASLRFEGSLNV 250


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  147 bits (357), Expect = 2e-36
 Identities = 70/168 (41%), Positives = 99/168 (58%)
 Frame = +1

Query: 310 ETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEI 489
           E   GK+VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+
Sbjct: 53  EAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAEL 112

Query: 490 VDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 669
            D VLD +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PA
Sbjct: 113 ADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPA 172

Query: 670 PXVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDIXRRNLDIERP 813
           P  S  VVEPYN+ L+ H  +E+SD  F +DNEA+  I    L I+ P
Sbjct: 173 PKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSP 220



 Score = 33.9 bits (74), Expect = 0.032
 Identities = 14/34 (41%), Positives = 18/34 (52%)
 Frame = +3

Query: 147 MRERISVHVGQAGVQIGNACWELYCLEHGIQPDG 248
           MRE + +  GQ G Q+G A W     EHG+   G
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAG 34


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 99.5 bits (237), Expect = 6e-22
 Identities = 54/166 (32%), Positives = 90/166 (54%), Gaps = 3/166 (1%)
 Frame = +1

Query: 325 KHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDL 498
           +++PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + 
Sbjct: 59  RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFED 117

Query: 499 VLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYP-APX 675
           ++D I + AD    L+GF + H             L+ERL+  Y KK    ++++P +  
Sbjct: 118 IMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQS 177

Query: 676 VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDIXRRNLDIERP 813
           VS  VV+PYNS+L       ++D   ++DN A+  I    L  + P
Sbjct: 178 VSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNP 223



 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 21/42 (50%), Positives = 27/42 (64%)
 Frame = +3

Query: 150 RERISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIG 275
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T G
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEG 44


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,489,114
Number of Sequences: 5004
Number of extensions: 69998
Number of successful extensions: 216
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 452494940
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)