Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP04_F_P12
(891 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 235 5e-63
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 9e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 3e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 124 2e-29
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.7
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.6
SPBC56F2.11 |met6||homoserine O-acetyltransferase|Schizosaccharo... 27 4.7
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.7
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 6.3
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 8.3
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 235 bits (576), Expect = 5e-63
Identities = 101/132 (76%), Positives = 117/132 (88%)
Frame = +1
Query: 136 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 315
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 316 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 495
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 496 RKEAESCDCLQG 531
R+EAE+CD LQG
Sbjct: 121 RREAEACDALQG 132
Score = 77.4 bits (182), Expect = 3e-15
Identities = 34/53 (64%), Positives = 41/53 (77%)
Frame = +3
Query: 711 LVENTDETYCVDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRXPG 869
LVEN+DET+C+DNEAL I TLK+ +P+Y DLNHLVS M+GVTT R PG
Sbjct: 192 LVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPG 244
Score = 70.9 bits (166), Expect = 2e-13
Identities = 31/38 (81%), Positives = 35/38 (92%)
Frame = +2
Query: 596 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQ 709
KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQ
Sbjct: 154 KIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQ 191
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 135 bits (326), Expect = 9e-33
Identities = 62/134 (46%), Positives = 85/134 (63%), Gaps = 2/134 (1%)
Frame = +1
Query: 136 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 309
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 310 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 489
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 490 VVRKEAESCDCLQG 531
+R+ A++C LQG
Sbjct: 121 KIRRIADNCSGLQG 134
Score = 51.6 bits (118), Expect = 1e-07
Identities = 22/49 (44%), Positives = 32/49 (65%)
Frame = +3
Query: 726 DETYCVDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRXPGT 872
D T+ VDNE+ YDIC R L + P+Y +LN L++ +S +T LR G+
Sbjct: 199 DCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGS 247
Score = 39.1 bits (87), Expect = 8e-04
Identities = 16/37 (43%), Positives = 25/37 (67%)
Frame = +2
Query: 596 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVH 706
++ EY + +SV P+P+VS +VVEPYN+ L+ H
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTH 192
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 130 bits (314), Expect = 3e-31
Identities = 59/138 (42%), Positives = 86/138 (62%), Gaps = 6/138 (4%)
Frame = +1
Query: 136 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 297
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 298 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 477
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 478 SVLDVVRKEAESCDCLQG 531
SVL+ +R+ A++C LQG
Sbjct: 121 SVLERIRRMADNCSGLQG 138
Score = 58.4 bits (135), Expect = 1e-09
Identities = 25/53 (47%), Positives = 36/53 (67%)
Frame = +3
Query: 714 VENTDETYCVDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRXPGT 872
++N+D T+ VDNEA YDIC R L + PTY +LN L++ +S +T LR G+
Sbjct: 199 LDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGS 251
Score = 38.7 bits (86), Expect = 0.001
Identities = 16/37 (43%), Positives = 25/37 (67%)
Frame = +2
Query: 596 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVH 706
++ EY + +SV P+P+VS +VVEPYN+ L+ H
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTH 196
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 124 bits (299), Expect = 2e-29
Identities = 52/133 (39%), Positives = 90/133 (67%), Gaps = 2/133 (1%)
Frame = +1
Query: 139 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 318
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 319 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 492
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 493 VRKEAESCDCLQG 531
+ +EA+ D L+G
Sbjct: 122 IDREADGSDSLEG 134
Score = 41.9 bits (94), Expect = 1e-04
Identities = 18/37 (48%), Positives = 27/37 (72%), Gaps = 1/37 (2%)
Frame = +2
Query: 596 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSV 703
++ + YP +I+ TYSV P S VSD VV+PYN+ L++
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLAL 192
Score = 41.9 bits (94), Expect = 1e-04
Identities = 23/53 (43%), Positives = 25/53 (47%)
Frame = +3
Query: 711 LVENTDETYCVDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRXPG 869
L N D +DN AL I L PT+ N LVS MS TT LR PG
Sbjct: 195 LTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPG 247
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +1
Query: 439 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 537
A+GH G ELV + D +RK++E+ L+ P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.6
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 175 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 276
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC56F2.11 |met6||homoserine
O-acetyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 489
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/37 (37%), Positives = 17/37 (45%)
Frame = +2
Query: 647 PSPKVSDTVVEPYNATLSVHQXS*KHRRNLLCRQRGS 757
P P T + P NA VH ++RR CR GS
Sbjct: 259 PYPDRLPTPLTPSNAHWVVHNEGNRNRRERPCRSNGS 295
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 370 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 471
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 6.3
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -2
Query: 512 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 375
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 8.3
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 391 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 522
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,190,651
Number of Sequences: 5004
Number of extensions: 61700
Number of successful extensions: 206
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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