Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP02_F_I20
         (950 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo...    63   6e-11
SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo...    62   1e-10
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce...    47   3e-06
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc...    28   2.2  
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c...    27   5.1  

>SPCC191.09c |gst1||glutathione S-transferase
           Gst1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 229

 Score = 62.9 bits (146), Expect = 6e-11
 Identities = 45/139 (32%), Positives = 71/139 (51%), Gaps = 8/139 (5%)
 Frame = +2

Query: 236 EQLKPEYLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKYAKGSSL-YPEDPKARAL 403
           EQ  PE+L LNP   VPTL+D   +  +IWES AI+ YL +KY     +  P D      
Sbjct: 40  EQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAILIYLADKYDTERKISLPRDHPEYYK 99

Query: 404 VDQRLYFDI---GTLYQRFSDY-FYPQVFAGAPADKAKNEKVQEALQLLDKFLEGQKYVA 571
           V Q L+F     G ++ +   +  Y Q    +   + +NE ++  L +L+  L+ + Y+ 
Sbjct: 100 VIQYLFFQASGQGIIWGQAGWFSVYHQELVISAITRYRNE-IKRVLGVLEDILKDRDYLV 158

Query: 572 GPNLTVADMSLIASVSSLE 628
               T+AD+S I+  + LE
Sbjct: 159 ANRFTIADLSFISWNNFLE 177


>SPCC965.07c |gst2||glutathione S-transferase
           Gst2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 230

 Score = 61.7 bits (143), Expect = 1e-10
 Identities = 46/135 (34%), Positives = 75/135 (55%), Gaps = 10/135 (7%)
 Frame = +2

Query: 233 GEQLKPEYLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKY--AKGSSLYPEDPKAR 397
           GEQ   E+L LNP   VPTLVD   +  +IWES AI+ YL +KY   +  SL  +DP+  
Sbjct: 39  GEQKCKEHLALNPNGRVPTLVDHKNNDYTIWESDAILIYLADKYDTDRKISLSFDDPEYY 98

Query: 398 ALVDQRLYFDI---GTLYQR--FSDYFYPQVFAGAPADKAKNEKVQEALQLLDKFLEGQK 562
            L+ Q L+F     G ++ +  + ++F+ +    A   + +NE ++  L +L+  L+ + 
Sbjct: 99  KLI-QYLFFQASGQGVIWGQAGWFNFFHHEPVVSA-VTRYRNE-IKRVLGVLEDILKDRD 155

Query: 563 YVAGPNLTVADMSLI 607
           Y+     T+AD+S I
Sbjct: 156 YLVANKYTIADLSFI 170


>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 242

 Score = 47.2 bits (107), Expect = 3e-06
 Identities = 40/157 (25%), Positives = 62/157 (39%), Gaps = 17/157 (10%)
 Frame = +2

Query: 248 PEYLKLNPQHTVPTLVDDGLSIWESRAIITYLVNKYAKGSSLYPEDPKARALVDQRLYFD 427
           P Y KL+P    P +VDDG++  ES AI+ +LV KY  G S  P +     L    L+  
Sbjct: 40  PAYTKLSPLGKSPIVVDDGVTYIESAAILEHLVRKY--GPSFKPSEEDVAELEKYELWMH 97

Query: 428 IG--------------TLYQRFSDYFYPQV---FAGAPADKAKNEKVQEALQLLDKFLEG 556
                            L    +  F+  +   F      K  +++    L  +D  L  
Sbjct: 98  FSEASLMPFIWASHVLDLSVNMTPIFFRYIVRQFVNGIKSKYLSKETFLNLDYIDNHLAS 157

Query: 557 QKYVAGPNLTVADMSLIASVSSLEXSDIDFKKYANVK 667
            +Y AG   T AD  +   + + +   +  K Y N+K
Sbjct: 158 NEYFAGEQFTAADPQMCFPIFAAQRDYLSQKPYKNIK 194


>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 721

 Score = 27.9 bits (59), Expect = 2.2
 Identities = 34/118 (28%), Positives = 52/118 (44%)
 Frame = +2

Query: 299 DGLSIWESRAIITYLVNKYAKGSSLYPEDPKARALVDQRLYFDIGTLYQRFSDYFYPQVF 478
           + LSI ESR  I  +  ++   S L+P D K  A  + RL F +G  + R +      + 
Sbjct: 263 NSLSI-ESRLTIANMTTEWGALSGLFPTDEKLLAWYEDRLKF-LGPNHPRVNRETLDAIK 320

Query: 479 AGAPADKAKNEKVQEALQLLDKFLEGQKYVAGPNLTVADMSLIASVSSLEXSDIDFKK 652
           A +P     +E    A  L+         V+GPN     + +  S ++LE  DI  KK
Sbjct: 321 A-SPI--LADEGAFYAKHLILDLSTLSPAVSGPN----SVKVYNSAATLEKKDILIKK 371


>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 589

 Score = 26.6 bits (56), Expect = 5.1
 Identities = 17/49 (34%), Positives = 23/49 (46%)
 Frame = +2

Query: 530 QLLDKFLEGQKYVAGPNLTVADMSLIASVSSLEXSDIDFKKYANVKEMV 676
           Q L   L+G KY AG        S IAS+ ++   +  F  YAN   M+
Sbjct: 505 QQLTTLLKGTKYSAG-----LSYSQIASIPNVSEKNFVFNAYANAIRMI 548


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,938,787
Number of Sequences: 5004
Number of extensions: 52078
Number of successful extensions: 140
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 485316198
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)