Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP01_F_N16
(838 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 184 9e-47
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 92 4e-19
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 92 4e-19
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 92 4e-19
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 92 4e-19
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 73 3e-13
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 66 3e-11
At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1) (... 43 2e-04
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 41 9e-04
At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2) (... 41 0.001
At4g15160.1 68417.m02327 protease inhibitor/seed storage/lipid t... 30 2.2
At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar ... 30 2.2
At4g02570.1 68417.m00351 cullin family protein similar to cullin... 28 8.8
At2g24450.1 68415.m02922 fasciclin-like arabinogalactan family p... 28 8.8
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 184 bits (447), Expect = 9e-47
Identities = 82/177 (46%), Positives = 122/177 (68%)
Frame = +2
Query: 308 MSAPHSTGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADT 487
+ APHS GT+I+ ++GGVV+GADSRT+TG Y+ANR +DK+T++TD +Y CRSGSAAD+
Sbjct: 5 LDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADS 64
Query: 488 QAIADIVTYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWDKKKGAQIY 667
Q ++D V Y L+ H ++ G P V+ +A + R L YN ++ L G++V GWDK +G +IY
Sbjct: 65 QVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNMLQTGLIVGGWDKYEGGKIY 124
Query: 668 SIPIGGMVQRQAVSIGGSGSSYXLWLR*RQFQAQYDQGRSQKFVTDTLTLAILRDGS 838
IP+GG V Q +IGGSGSSY + ++ + +++ V ++LAI RDG+
Sbjct: 125 GIPLGGTVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGA 181
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 92.3 bits (219), Expect = 4e-19
Identities = 52/173 (30%), Positives = 94/173 (54%), Gaps = 4/173 (2%)
Frame = +2
Query: 326 TGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADI 505
TGT+I+ F GV++GAD+R T G +A++ +K+ + IYCC +G+AADT+A+ D+
Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDM 97
Query: 506 VTYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWDKKKGAQIYSIPIGG 685
V+ L H+ + G V TA + ++ ++Y+ + A +++ G D G +++I G
Sbjct: 98 VSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVLGGVD-ITGPHLHTIYPHG 156
Query: 686 MVQRQAVSIGGSGSSYXLWLR*RQFQAQYDQGRSQ----KFVTDTLTLAILRD 832
+ GSGS + + F+A+Y +G ++ K V + + I D
Sbjct: 157 STDTLPFATMGSGSLAAMSV----FEAKYKEGLTRDEGIKLVAEAICSGIFND 205
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 92.3 bits (219), Expect = 4e-19
Identities = 52/173 (30%), Positives = 94/173 (54%), Gaps = 4/173 (2%)
Frame = +2
Query: 326 TGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADI 505
TGT+I+ F GV++GAD+R T G +A++ +K+ + IYCC +G+AADT+A+ D+
Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDM 97
Query: 506 VTYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWDKKKGAQIYSIPIGG 685
V+ L H+ + G V TA + ++ ++Y+ + A +++ G D G +++I G
Sbjct: 98 VSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVLGGVD-ITGPHLHTIYPHG 156
Query: 686 MVQRQAVSIGGSGSSYXLWLR*RQFQAQYDQGRSQ----KFVTDTLTLAILRD 832
+ GSGS + + F+A+Y +G ++ K V + + I D
Sbjct: 157 STDTLPFATMGSGSLAAMSV----FEAKYKEGLTRDEGIKLVAEAICSGIFND 205
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 92.3 bits (219), Expect = 4e-19
Identities = 52/173 (30%), Positives = 95/173 (54%), Gaps = 4/173 (2%)
Frame = +2
Query: 326 TGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADI 505
TGT+I+ F GV++GAD+R T G +A++ +K+ + IYCC +G+AADT+A+ D+
Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDM 97
Query: 506 VTYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWDKKKGAQIYSIPIGG 685
V+ L H+ + G V TA + ++ ++Y+ + A +++ G D G +++I G
Sbjct: 98 VSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVLGGVD-ITGPHLHTIYPHG 156
Query: 686 MVQRQAVSIGGSGSSYXLWLR*RQFQAQYDQGRSQ----KFVTDTLTLAILRD 832
+ GSGS + + F+A+Y +G ++ K V +++ I D
Sbjct: 157 STDTLPFATMGSGSLAAMSV----FEAKYKEGLTRDEGIKLVAESICSGIFND 205
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 92.3 bits (219), Expect = 4e-19
Identities = 52/173 (30%), Positives = 95/173 (54%), Gaps = 4/173 (2%)
Frame = +2
Query: 326 TGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADI 505
TGT+I+ F GV++GAD+R T G +A++ +K+ + IYCC +G+AADT+A+ D+
Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDM 97
Query: 506 VTYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWDKKKGAQIYSIPIGG 685
V+ L H+ + G V TA + ++ ++Y+ + A +++ G D G +++I G
Sbjct: 98 VSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVLGGVD-ITGPHLHTIYPHG 156
Query: 686 MVQRQAVSIGGSGSSYXLWLR*RQFQAQYDQGRSQ----KFVTDTLTLAILRD 832
+ GSGS + + F+A+Y +G ++ K V +++ I D
Sbjct: 157 STDTLPFATMGSGSLAAMSV----FEAKYKEGLTRDEGIKLVAESICSGIFND 205
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 72.5 bits (170), Expect = 3e-13
Identities = 47/174 (27%), Positives = 81/174 (46%), Gaps = 1/174 (0%)
Frame = +2
Query: 320 HSTGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIA 499
H+ GT+ +A F GGV++ ADSR + G YI+++ K+ +I + +G AAD Q
Sbjct: 54 HAKGTTTLAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWH 113
Query: 500 DIVTYHLNFHKMELGDPPLVETAAAIFRELCYNYRD-SLMAGILVAGWDKKKGAQIYSIP 676
+ H++ V A+ + + Y+YR L G ++AGWD+ Y
Sbjct: 114 RNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDN 173
Query: 677 IGGMVQRQAVSIGGSGSSYXLWLR*RQFQAQYDQGRSQKFVTDTLTLAILRDGS 838
GG ++ S+ GSGS Y + ++ + + ++ A RDG+
Sbjct: 174 EGGRLKGDRFSV-GSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGA 226
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 66.1 bits (154), Expect = 3e-11
Identities = 45/171 (26%), Positives = 78/171 (45%), Gaps = 1/171 (0%)
Frame = +2
Query: 329 GTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADIV 508
GT+ +A F GV++ ADSR + G YI+++ K+ +I + +G AAD Q +
Sbjct: 57 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 116
Query: 509 TYHLNFHKMELGDPPLVETAAAIFRELCYNYRD-SLMAGILVAGWDKKKGAQIYSIPIGG 685
H++ V A+ + + Y+YR L G ++AGWD+ Y GG
Sbjct: 117 GIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGG 176
Query: 686 MVQRQAVSIGGSGSSYXLWLR*RQFQAQYDQGRSQKFVTDTLTLAILRDGS 838
++ S+ GSGS Y + ++ + + ++ A RDG+
Sbjct: 177 RLKGDRFSV-GSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGA 226
>At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1)
(PRC9) identical to GB:AAC32057 from [Arabidopsis
thaliana] (Genetics (1998) 149 (2), 677-692); identical
to cDNA proteasome subunit prc9 GI:2511583
Length = 250
Score = 43.2 bits (97), Expect = 2e-04
Identities = 35/147 (23%), Positives = 59/147 (40%), Gaps = 6/147 (4%)
Frame = +2
Query: 308 MSAPHSTGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADT 487
M A + G++I DG V+IG + + T+ + +K+ KI D + C +G +D
Sbjct: 25 MEAIGNAGSAIGILSKDGVVLIG-EKKVTSKLLQTSTSAEKMYKIDDHVACAVAGIMSDA 83
Query: 488 QAIADIVTYHLNFHKMELGDPPLVETAAAIFRELCYNYRD-----SLMAGILVAGWDKKK 652
+ + + +P VE + Y L AGWDK
Sbjct: 84 NILINTARVQAQRYTFMYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKHH 143
Query: 653 GAQIY-SIPIGGMVQRQAVSIGGSGSS 730
G Q+Y S P G +A ++G + +
Sbjct: 144 GFQLYMSDPSGNYGGWKAAAVGANNQA 170
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 41.1 bits (92), Expect = 9e-04
Identities = 34/143 (23%), Positives = 60/143 (41%), Gaps = 1/143 (0%)
Frame = +2
Query: 305 WMSAPHSTGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAAD 484
W ++ GT + D VI AD+R +TG I +R K+ K+ D+ SG AD
Sbjct: 8 WSPYDNNGGTCVAIAGSDY-CVIAADTRMSTGYSILSRDYSKIHKLADRAVLSSSGFQAD 66
Query: 485 TQAIADIV-TYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWDKKKGAQ 661
+A+ ++ + HL + A + L + A ++ G D++
Sbjct: 67 VKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLDEEGKGC 126
Query: 662 IYSIPIGGMVQRQAVSIGGSGSS 730
+++ G +R GSGS+
Sbjct: 127 VFTYDAVGSYERVGYGAQGSGST 149
>At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2)
(PRCGA) identical to SP|O24633 Proteasome subunit beta
type 2-2 (EC 3.4.25.1) (20S proteasome alpha subunit D2)
{Arabidopsis thaliana}, cDNA proteasome subunit prcga
GI:2511571
Length = 199
Score = 40.7 bits (91), Expect = 0.001
Identities = 32/139 (23%), Positives = 56/139 (40%), Gaps = 2/139 (1%)
Frame = +2
Query: 356 DGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADIVTYHLNFHKM 535
+G ++ AD+ + DK+ + SG D + V +++ ++
Sbjct: 10 NGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYVQKNVSLYQF 69
Query: 536 ELGDPPLVETAAAIFR-ELCYNYRDS-LMAGILVAGWDKKKGAQIYSIPIGGMVQRQAVS 709
G P AA R EL R + IL+AG+DK+ GA +Y I + +
Sbjct: 70 RNGIPLSTAAAANFTRGELATALRKNPYSVNILMAGYDKEAGASLYYIDYIATLHKVDKG 129
Query: 710 IGGSGSSYXLWLR*RQFQA 766
G GS + L R +++
Sbjct: 130 AFGYGSYFSLSTMDRHYRS 148
>At4g15160.1 68417.m02327 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to
SP|Q00451|PRF1_LYCES 36.4 kDa proline-rich protein
Lycopersicon esculentum, proline-rich cell wall protein
[Medicago sativa] GI:3818416; contains Pfam profile
PF00234 Protease inhibitor/seed storage/LTP family
Length = 428
Score = 29.9 bits (64), Expect = 2.2
Identities = 19/60 (31%), Positives = 32/60 (53%), Gaps = 1/60 (1%)
Frame = +2
Query: 554 LVETAAAIFRELCYNYRDSLMAGILVAGWDKKKGAQIY-SIPIGGMVQRQAVSIGGSGSS 730
L++T++++ E Y D + VAGWD+ G Q+Y S P G QA ++G + +
Sbjct: 337 LLQTSSSM--EKMYKIDDHVACA--VAGWDRNHGFQLYMSDPSGNYGGWQAAAVGANNQA 392
>At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 212
Score = 29.9 bits (64), Expect = 2.2
Identities = 15/49 (30%), Positives = 29/49 (59%)
Frame = +2
Query: 236 LLPTTSKMATSVINTYSDPCLPEWMSAPHSTGTSIMACEFDGGVVIGAD 382
++PTT + +++I + S C W++ PH TG+ ++ E V++ AD
Sbjct: 5 MIPTTI-LLSALIFSLSPICEAVWLTVPH-TGSKCVSEEIQSNVIVLAD 51
>At4g02570.1 68417.m00351 cullin family protein similar to cullin 3
[Homo sapiens] GI:3639052; contains Pfam profile
PF00888: Cullin family
Length = 738
Score = 27.9 bits (59), Expect = 8.8
Identities = 16/40 (40%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = +2
Query: 548 PPLVETAAAIFRELCYNYRDSLMAGILVAGWDK-KKGAQI 664
PPL E FR+L YN S + ++A DK ++G QI
Sbjct: 126 PPLNEVGLTCFRDLVYNELHSKVKQAVIALVDKEREGEQI 165
>At2g24450.1 68415.m02922 fasciclin-like arabinogalactan family
protein similar to fasciclin-like
arabinogalactan-protein 1 [Arabidopsis thaliana]
gi|13377776|gb|AAK20857
Length = 280
Score = 27.9 bits (59), Expect = 8.8
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = -2
Query: 465 RQQYIWSVIFVSLSVTRLAM*APVVVRESAPMTTPPSNS 349
R Y SV+ +S+ + + +PV V PM++PP+ S
Sbjct: 155 RNPYNLSVVQISMPIVAPGLGSPVKVPPPPPMSSPPAPS 193
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,775,449
Number of Sequences: 28952
Number of extensions: 373357
Number of successful extensions: 765
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 746
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 760
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1931371200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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