Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP01_F_M22
(891 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 236 4e-63
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 227 1e-60
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 124 2e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 106 4e-24
SPCC613.10 |qcr2||ubiquinol-cytochrome-c reductase complex core ... 26 8.3
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.3
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.3
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 236 bits (577), Expect = 4e-63
Identities = 101/139 (72%), Positives = 119/139 (85%)
Frame = +3
Query: 144 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 323
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 324 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 503
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 504 RIRKLADQCTGLQGFLIFH 560
+IR++AD C+GLQGFL+FH
Sbjct: 121 KIRRIADNCSGLQGFLVFH 139
Score = 54.8 bits (126), Expect = 2e-08
Identities = 22/29 (75%), Positives = 28/29 (96%)
Frame = +1
Query: 622 DYGKKSKLEFAIYPAPQVSTAVVEPYNSI 708
+Y KKSKL+F++YPAPQVST+VVEPYNS+
Sbjct: 160 EYTKKSKLQFSVYPAPQVSTSVVEPYNSV 188
Score = 39.9 bits (89), Expect = 5e-04
Identities = 19/26 (73%), Positives = 20/26 (76%)
Frame = +3
Query: 792 NLDIERPTYTNLNRLIGXXVSSXPAS 869
NLDIERP+Y NLNRLI VSS AS
Sbjct: 216 NLDIERPSYENLNRLIAQVVSSITAS 241
Score = 37.9 bits (84), Expect = 0.002
Identities = 14/18 (77%), Positives = 16/18 (88%)
Frame = +2
Query: 737 SDCAFMVDNEAIYDICRR 790
+DC FMVDNE+ YDICRR
Sbjct: 198 ADCTFMVDNESCYDICRR 215
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 227 bits (556), Expect = 1e-60
Identities = 102/144 (70%), Positives = 121/144 (84%), Gaps = 5/144 (3%)
Frame = +3
Query: 144 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 308
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 309 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 488
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 489 DLVLDRIRKLADQCTGLQGFLIFH 560
D VL+RIR++AD C+GLQGFL+FH
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFH 143
Score = 56.0 bits (129), Expect = 7e-09
Identities = 22/29 (75%), Positives = 28/29 (96%)
Frame = +1
Query: 622 DYGKKSKLEFAIYPAPQVSTAVVEPYNSI 708
+YGKKS L+F++YPAPQVST+VVEPYNS+
Sbjct: 164 EYGKKSNLQFSVYPAPQVSTSVVEPYNSV 192
Score = 41.5 bits (93), Expect = 2e-04
Identities = 20/26 (76%), Positives = 20/26 (76%)
Frame = +3
Query: 792 NLDIERPTYTNLNRLIGXXVSSXPAS 869
NLDIERPTY NLNRLI VSS AS
Sbjct: 220 NLDIERPTYENLNRLIAQVVSSITAS 245
Score = 40.7 bits (91), Expect = 3e-04
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +2
Query: 734 HSDCAFMVDNEAIYDICRR 790
+SDC FMVDNEA YDICRR
Sbjct: 201 NSDCTFMVDNEACYDICRR 219
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 124 bits (298), Expect = 2e-29
Identities = 59/139 (42%), Positives = 81/139 (58%)
Frame = +3
Query: 144 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 323
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 324 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 503
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 504 RIRKLADQCTGLQGFLIFH 560
+R+ A+ C LQGF + H
Sbjct: 119 VVRREAEACDALQGFQLTH 137
Score = 30.7 bits (66), Expect = 0.29
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = +1
Query: 622 DYGKKSKLEFAIYPAPQVSTAVVEPYNS 705
+Y + F++ PAP+ S VVEPYN+
Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNA 185
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 106 bits (255), Expect = 4e-24
Identities = 54/140 (38%), Positives = 84/140 (60%), Gaps = 2/140 (1%)
Frame = +3
Query: 147 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 326
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 327 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 500
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 501 DRIRKLADQCTGLQGFLIFH 560
D I + AD L+GF + H
Sbjct: 120 DMIDREADGSDSLEGFSLLH 139
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Frame = +1
Query: 625 YGKKSKLEFAIYPAPQ-VSTAVVEPYNSI 708
Y KK ++++P Q VS VV+PYNS+
Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSL 189
>SPCC613.10 |qcr2||ubiquinol-cytochrome-c reductase complex core
protein Qcr2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 426
Score = 25.8 bits (54), Expect = 8.3
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = +3
Query: 726 PXSTLTVLSWSTMKPSMTSAAGNLDIERPTYTNL 827
P S V+S KP+ T A GNLD+ P Y L
Sbjct: 394 PASISKVVSSLLAKPASTVAVGNLDV-LPYYDEL 426
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.3
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 223 STASSLMARCPQTRPSGVETILSTLSSARPELAST 327
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.3
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 242 WPDAHRQDHRGWRRFFQHFLQR 307
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,519,610
Number of Sequences: 5004
Number of extensions: 71841
Number of successful extensions: 209
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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