BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fdpeP01_F_M12 (917 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz... 69 9e-13 SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 61 2e-10 SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 48 2e-06 SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 48 2e-06 SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb... 45 2e-05 SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 41 2e-04 SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 36 0.008 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 31 0.23 SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 31 0.30 SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomy... 27 4.9 SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 27 4.9 SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase Prs1|Schizo... 26 6.5 SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces pom... 26 8.6 SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 26 8.6 >SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schizosaccharomyces pombe|chr 1|||Manual Length = 307 Score = 68.9 bits (161), Expect = 9e-13 Identities = 32/77 (41%), Positives = 52/77 (67%) Frame = +3 Query: 120 VGNNEVELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSV 299 +GN+ + E YDRQIRLWG ++Q+ L+ ++VL+I S L EIAKN++L+G+ + Sbjct: 1 MGNHNINAEEIAL--YDRQIRLWGFNAQQALKQSRVLLITASPLANEIAKNLVLSGIGKL 58 Query: 300 CLLDNEKLKQIDLYSQF 350 C+LD+ + + D+ QF Sbjct: 59 CVLDSMTVYEKDVEEQF 75 Score = 66.5 bits (155), Expect = 5e-12 Identities = 37/119 (31%), Positives = 61/119 (51%) Frame = +1 Query: 280 SPV*KVCVC*TMRN*NKSICTPNFCVLLXKIGVNRAEGSLERARGLNPMVDVTSHTKGVD 459 S + K+CV +M K + F + IG RA ++ LNP+V++ + T + Sbjct: 53 SGIGKLCVLDSMTVYEKDV-EEQFFIEASDIGQLRANVFKKKLHELNPLVEIDTDTSLIS 111 Query: 460 ELPDSFFTEFDVVCATGLKQEQFERINNACRDSNKKFICGDVWGTYGYMFSDLVDHEYS 636 E+ + ++F +V AT L E+F RIN R N F +G YG+ F DL++H ++ Sbjct: 112 EIDEGKISKFSMVIATQLDYEEFCRINELTRICNASFYATSCFGLYGFAFCDLINHNFA 170 >SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme |Schizosaccharomyces pombe|chr 2|||Manual Length = 1012 Score = 61.3 bits (142), Expect = 2e-10 Identities = 31/62 (50%), Positives = 41/62 (66%) Frame = +3 Query: 165 YDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYS 344 Y RQ+ + G ++ K++ + VLIIG GLG EIAKNV L GVKSV L D + + DL S Sbjct: 20 YSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDLSS 79 Query: 345 QF 350 Q+ Sbjct: 80 QY 81 >SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schizosaccharomyces pombe|chr 2|||Manual Length = 628 Score = 48.0 bits (109), Expect = 2e-06 Identities = 19/54 (35%), Positives = 38/54 (70%) Frame = +3 Query: 192 LDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFL 353 +++ + ++AKVL++G G+G E+ KN++++GVK V ++D + + +L QFL Sbjct: 17 VEALRNFKSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFL 70 >SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 500 Score = 47.6 bits (108), Expect = 2e-06 Identities = 19/57 (33%), Positives = 36/57 (63%) Frame = +3 Query: 144 SEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDN 314 + A+ ++YDRQ+RLW + Q + + V ++ + +G E KN+IL G+ S ++D+ Sbjct: 3 TSAKMQKYDRQVRLWKAEGQNAIEKSHVCLLYANTVGCEALKNLILPGIGSFAVVDD 59 >SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 44.8 bits (101), Expect = 2e-05 Identities = 24/78 (30%), Positives = 45/78 (57%), Gaps = 2/78 (2%) Frame = +3 Query: 126 NNEVELSEAEAEQYDRQIRLW--GLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSV 299 +N +ELS E +Y RQ+ L GL Q L+ + VL+IG GLG + ++ G+ ++ Sbjct: 11 SNGLELSLDEYSRYGRQMLLSEIGLPGQLSLKRSSVLVIGAGGLGCPAMQYLVAAGIGTL 70 Query: 300 CLLDNEKLKQIDLYSQFL 353 ++D + + + +L+ Q + Sbjct: 71 GIMDGDVVDKSNLHRQII 88 >SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 41.1 bits (92), Expect = 2e-04 Identities = 19/53 (35%), Positives = 34/53 (64%) Frame = +3 Query: 195 DSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFL 353 ++ K ++K+LIIG GLG EI K++ L+G + + ++D + + +L QFL Sbjct: 37 ETLKSAFSSKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQFL 89 >SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 35.9 bits (79), Expect = 0.008 Identities = 15/55 (27%), Positives = 33/55 (60%) Frame = +3 Query: 159 EQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKL 323 EQ R +G D +RLR + V+++G G+G+ + + +GV+ + ++D +++ Sbjct: 107 EQLARNYAFFGEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIRIVDFDQV 161 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 31.1 bits (67), Expect = 0.23 Identities = 18/52 (34%), Positives = 28/52 (53%) Frame = -3 Query: 459 IYALSMTCDIDHRIQASSSFQRTLCSIYADLVQEDTEIGSTDRFVSVSHCLT 304 + +L D ++R QA S LC IYA ++Q+ + I T F S+ H L+ Sbjct: 3639 VSSLGYDHDFENRAQAVSM----LCQIYAIVIQKHSSISPTASFQSIGHELS 3686 >SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 30.7 bits (66), Expect = 0.30 Identities = 19/60 (31%), Positives = 33/60 (55%), Gaps = 3/60 (5%) Frame = +3 Query: 141 LSEAEAEQYDRQIRLWGLDSQ---KRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLD 311 LSE+ A + + W L Q R++ +K L++G LG +A+N++ GV+ V +D Sbjct: 308 LSES-ASTLNLSLMRWRLVPQLDLDRIQNSKCLLLGAGTLGCGVARNLLSWGVRHVTFVD 366 >SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1019 Score = 26.6 bits (56), Expect = 4.9 Identities = 19/73 (26%), Positives = 29/73 (39%), Gaps = 3/73 (4%) Frame = +1 Query: 586 WGTYGYMFSDLVDHEYSEEIVQHKATKRGPDDEEKNARETVSITVKRRAIYVPLQ---NA 756 WG G D D EE+ +++A+ P DEE+ E S Y + Sbjct: 929 WGFLGAPSDDEGDDSV-EEVSEYEASDADPSDEEEEESEEYSEDASEEDGYSESEVEDEE 987 Query: 757 LSADWNSPEMRSR 795 DW+ E ++R Sbjct: 988 SGEDWDELERKAR 1000 >SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit Cct6|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 26.6 bits (56), Expect = 4.9 Identities = 12/19 (63%), Positives = 13/19 (68%) Frame = +3 Query: 285 GVKSVCLLDNEKLKQIDLY 341 G SVCLL E LKQ +LY Sbjct: 89 GTTSVCLLVGELLKQAELY 107 >SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase Prs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 26.2 bits (55), Expect = 6.5 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +3 Query: 249 LGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPXQDRRKS 383 LG I I+T SVC + ++++LYSQ CP D ++ Sbjct: 240 LGHIIDDEEIITTPASVC--SEDYAQEVNLYSQGGCPSDDDEEEN 282 >SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 561 Score = 25.8 bits (54), Expect = 8.6 Identities = 13/49 (26%), Positives = 21/49 (42%) Frame = +1 Query: 334 ICTPNFCVLLXKIGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFF 480 I + C++ G E +L+R NP+ + +HT D FF Sbjct: 108 ITAKHTCIVYNSTGAIGVEEALQRQYPENPIFSLITHTPVSQRSVDEFF 156 >SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit Cct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 25.8 bits (54), Expect = 8.6 Identities = 20/63 (31%), Positives = 27/63 (42%), Gaps = 1/63 (1%) Frame = +1 Query: 361 LXKIGVNRAEGSLERARG-LNPMVDVTSHTKGVDELPDSFFTEFDVVCATGLKQEQFERI 537 L KI +LER + LN +V TKG+D+L E + K+E RI Sbjct: 265 LEKIREREVMITLERVKKILNAGANVILTTKGIDDLCLKSIIEAGAMAVRRCKKEDLRRI 324 Query: 538 NNA 546 A Sbjct: 325 AKA 327 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,547,554 Number of Sequences: 5004 Number of extensions: 70010 Number of successful extensions: 217 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 209 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 217 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 466510270 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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