Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP01_F_I24
(898 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 235 5e-63
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 9e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 3e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 124 2e-29
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 1.2
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.7
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.6
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.8
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 6.3
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 8.4
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 235 bits (576), Expect = 5e-63
Identities = 101/132 (76%), Positives = 117/132 (88%)
Frame = +2
Query: 134 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 313
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 314 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 493
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 494 RKEAESCDCLQG 529
R+EAE+CD LQG
Sbjct: 121 RREAEACDALQG 132
Score = 106 bits (255), Expect = 4e-24
Identities = 53/92 (57%), Positives = 60/92 (65%)
Frame = +3
Query: 519 ASRGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLS 698
A +GFQ KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS
Sbjct: 129 ALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLS 188
Query: 699 VHQLVENTDETYCIDNEALYDICFRTLNYPHP 794
+HQLVEN+DET+CIDNEAL I TL P
Sbjct: 189 MHQLVENSDETFCIDNEALSSIFANTLKIKSP 220
Score = 61.7 bits (143), Expect = 1e-10
Identities = 26/38 (68%), Positives = 32/38 (84%)
Frame = +2
Query: 779 KLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKL 892
K+ +P+Y DLNHLVS M+GVTT RFPG+LN+DLRKL
Sbjct: 216 KIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKL 253
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 135 bits (326), Expect = 9e-33
Identities = 62/134 (46%), Positives = 85/134 (63%), Gaps = 2/134 (1%)
Frame = +2
Query: 134 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 307
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 308 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 487
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 488 VVRKEAESCDCLQG 529
+R+ A++C LQG
Sbjct: 121 KIRRIADNCSGLQG 134
Score = 65.3 bits (152), Expect = 1e-11
Identities = 28/67 (41%), Positives = 43/67 (64%)
Frame = +3
Query: 594 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 773
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
Query: 774 TLNYPHP 794
L+ P
Sbjct: 216 NLDIERP 222
Score = 37.5 bits (83), Expect = 0.003
Identities = 15/34 (44%), Positives = 22/34 (64%)
Frame = +2
Query: 782 LSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADL 883
+ P+Y +LN L++ +S +T LRF G LN DL
Sbjct: 219 IERPSYENLNRLIAQVVSSITASLRFEGSLNVDL 252
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 130 bits (314), Expect = 3e-31
Identities = 59/138 (42%), Positives = 86/138 (62%), Gaps = 6/138 (4%)
Frame = +2
Query: 134 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 295
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 296 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 475
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 476 SVLDVVRKEAESCDCLQG 529
SVL+ +R+ A++C LQG
Sbjct: 121 SVLERIRRMADNCSGLQG 138
Score = 70.1 bits (164), Expect = 4e-13
Identities = 30/67 (44%), Positives = 45/67 (67%)
Frame = +3
Query: 594 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 773
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219
Query: 774 TLNYPHP 794
L+ P
Sbjct: 220 NLDIERP 226
Score = 39.5 bits (88), Expect = 6e-04
Identities = 16/36 (44%), Positives = 23/36 (63%)
Frame = +2
Query: 782 LSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRK 889
+ PTY +LN L++ +S +T LRF G LN DL +
Sbjct: 223 IERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNE 258
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 124 bits (299), Expect = 2e-29
Identities = 52/133 (39%), Positives = 90/133 (67%), Gaps = 2/133 (1%)
Frame = +2
Query: 137 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 316
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 317 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 490
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 491 VRKEAESCDCLQG 529
+ +EA+ D L+G
Sbjct: 122 IDREADGSDSLEG 134
Score = 57.2 bits (132), Expect = 3e-09
Identities = 28/68 (41%), Positives = 41/68 (60%), Gaps = 1/68 (1%)
Frame = +3
Query: 594 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICF 770
++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAA 215
Query: 771 RTLNYPHP 794
L+ +P
Sbjct: 216 DRLHTQNP 223
Score = 37.9 bits (84), Expect = 0.002
Identities = 17/31 (54%), Positives = 20/31 (64%)
Frame = +2
Query: 791 PTYGDLNHLVSLTMSGVTTCLRFPGQLNADL 883
PT+ N LVS MS TT LR+PG +N DL
Sbjct: 223 PTFHQQNQLVSTVMSASTTTLRYPGYMNNDL 253
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/44 (40%), Positives = 24/44 (54%)
Frame = +2
Query: 662 GHSSRTVQRHTFSSSIS*KHRRNLLYRQRGSLRYLLPHAKLSTP 793
G+ RT +R F S ++R +LL R SLR L P A +TP
Sbjct: 70 GNRDRTTERSAFRS----RYRGSLLNRNSPSLRSLSPPATPATP 109
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +2
Query: 437 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 535
A+GH G ELV + D +RK++E+ L+ P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.6
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 173 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 274
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.8
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 368 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 469
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 6.3
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -2
Query: 510 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 373
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 8.4
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 389 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 520
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,380,760
Number of Sequences: 5004
Number of extensions: 66986
Number of successful extensions: 213
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 452494940
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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