BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP01_F_I20
(931 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1259.03 |rpa12||DNA-directed RNA polymerase complex I subuni... 75 2e-14
SPAC22A12.05 |rpc11||DNA-directed RNA polymerase III complex sub... 52 1e-07
SPAC20H4.03c |tfs1||transcription elongation factor TFIIS |Schiz... 42 1e-04
SPAPYUG7.04c |rpb9||DNA-directed RNA polymerase II complex subun... 32 0.10
SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces pomb... 26 6.6
SPAC144.06 |apl5||AP-3 adaptor complex subunit Apl5 |Schizosacch... 26 8.7
>SPCC1259.03 |rpa12||DNA-directed RNA polymerase complex I subunit
Rpa12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 119
Score = 74.5 bits (175), Expect = 2e-14
Identities = 38/115 (33%), Positives = 62/115 (53%), Gaps = 4/115 (3%)
Frame = +3
Query: 90 STAFCARCGSILPLLQEFGSVKCYTCKAHYEADNYSKMKFQYTIHFNTI--SVITNENIL 263
S FC+ CG++L + C C++ Y ++ ++ + + + ++ +I+
Sbjct: 6 SLIFCSECGNLLESTTAQWTT-CDQCQSVYPSEQFANLVVETKSSASAFPSALKLKHSIV 64
Query: 264 HTDGP--EGPVVERKCAKCGYDRMSYATLQLRSADEGQTVFYTCIKCKYKETENS 422
+ E +E KC KCG D M++ TLQLRSADEG TVFY C +C YK + N+
Sbjct: 65 QVESQKEEAATIEEKCPKCGNDHMTFHTLQLRSADEGSTVFYECPRCAYKFSTNN 119
>SPAC22A12.05 |rpc11||DNA-directed RNA polymerase III complex
subunit Rpc11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 109
Score = 52.0 bits (119), Expect = 1e-07
Identities = 31/110 (28%), Positives = 50/110 (45%), Gaps = 3/110 (2%)
Frame = +3
Query: 99 FCARCGS-ILPLLQEFG--SVKCYTCKAHYEADNYSKMKFQYTIHFNTISVITNENILHT 269
FC CG+ ++ + E G + C TC H+ + + ++ V+ E +
Sbjct: 3 FCPTCGNHLIVAVDEEGRNAFDCRTCPYHFPISTFLYSRHEFA-QKEVDDVLGGEEAFES 61
Query: 270 DGPEGPVVERKCAKCGYDRMSYATLQLRSADEGQTVFYTCIKCKYKETEN 419
+ E KC +R + LQ+RSADE + FY C KCK++ EN
Sbjct: 62 NQQTEVTCEN--TKCDNNRAYFFQLQIRSADEPMSTFYRCTKCKFQWREN 109
>SPAC20H4.03c |tfs1||transcription elongation factor TFIIS
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 293
Score = 41.9 bits (94), Expect = 1e-04
Identities = 21/55 (38%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Frame = +3
Query: 243 ITNENILHTDG--PEGPVVER-KCAKCGYDRMSYATLQLRSADEGQTVFYTCIKC 398
+ EN+ H G P+ V + C KC ++SY +Q RSADE T F C C
Sbjct: 232 LEQENLFHAQGAKPQKAVTDLFTCGKCKQKKVSYYQMQTRSADEPMTTFCECTVC 286
>SPAPYUG7.04c |rpb9||DNA-directed RNA polymerase II complex subunit
Rpb9 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 113
Score = 32.3 bits (70), Expect = 0.10
Identities = 20/104 (19%), Positives = 41/104 (39%), Gaps = 2/104 (1%)
Frame = +3
Query: 99 FCARCGSIL-PLLQEFGSVKCYTCK-AHYEADNYSKMKFQYTIHFNTISVITNENILHTD 272
+C C ++L P + V C+ Y + +++ + + + T + TD
Sbjct: 6 YCIECNNMLYPREDKVDRVLRLACRNCDYSEIAATSKVYRHELQSSNVENTTVSHDASTD 65
Query: 273 GPEGPVVERKCAKCGYDRMSYATLQLRSADEGQTVFYTCIKCKY 404
P P +++C +C + R D T+ Y C+ C +
Sbjct: 66 -PTLPRSDKECPRCHQHEAVFYQTHSRRGDTMMTLIYVCVHCGF 108
>SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 983
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = +3
Query: 426 RLLYTSFKVVKGKLGNRIIVSESLYAKIS 512
RLL T ++K GNR++ S ++ K++
Sbjct: 146 RLLITLHHIIKAMAGNRLLRSRQIFYKLA 174
>SPAC144.06 |apl5||AP-3 adaptor complex subunit Apl5
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 834
Score = 25.8 bits (54), Expect = 8.7
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -2
Query: 663 IFKTWFLRLISIEFTV*KLSFQEMELKLRTSYMVINFN 550
+ K W +++S F L+FQ+ME++ R S I FN
Sbjct: 541 LVKLWVEKIVS--FIEQFLNFQDMEIQRRASEFYILFN 576
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,186,264
Number of Sequences: 5004
Number of extensions: 62162
Number of successful extensions: 129
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 471335896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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