BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fdpeP01_F_A03 (877 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 250 2e-67 SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 113 2e-26 SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 74 2e-14 SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 62 1e-10 SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 53 5e-08 SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 46 9e-06 SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 39 0.001 SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 34 0.031 SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 33 0.053 SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 32 0.12 SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 28 2.0 SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 27 3.5 >SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 267 Score = 250 bits (612), Expect = 2e-67 Identities = 114/195 (58%), Positives = 145/195 (74%) Frame = +2 Query: 197 GFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQK 376 GF FE +QRN L +KGFP PKAT TGTTIVG+I D ++LGADTRAT +++DKNC+K Sbjct: 9 GFDFEYYQRNLLLQEKGFPTPKATSTGTTIVGVIAKDCIVLGADTRATAGPIIADKNCKK 68 Query: 377 IHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGH 556 +H ++ N++C GAGTAADTE T ++S +EL L+T R V TA T+LK+ LFRYQGH Sbjct: 69 LHLISPNIWCAGAGTAADTEFVTSMISSNIELHSLYTNRKPRVVTALTMLKQHLFRYQGH 128 Query: 557 IGAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKK 736 IGA LVLGG D GPH++ I HGS DKLPY +GSGSLAA++V E ++ D+ E + Sbjct: 129 IGAYLVLGGYDCKGPHLFTIAAHGSSDKLPYVALGSGSLAAISVLETKYQPDLERHEAME 188 Query: 737 LVRDAIAAGIFNDLG 781 LV++AI AGIFNDLG Sbjct: 189 LVKEAIEAGIFNDLG 203 >SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 113 bits (273), Expect = 2e-26 Identities = 58/170 (34%), Positives = 94/170 (55%), Gaps = 1/170 (0%) Frame = +2 Query: 269 KTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQ 448 + GTTI + Y DGVIL AD+R T ++++ K+ L N++CC +G+AADT+ Sbjct: 22 RMGTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVAD 81 Query: 449 SVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVD-RTGPHIYCIYPH 625 + L + R+ G V TAATL M ++ + + A L++ G D +TG +Y I Sbjct: 82 LLKYYLSMYRIQFGHDPSVHTAATLASEMCYQNKNMLSAGLIVAGYDEKTGGDVYSIPLG 141 Query: 626 GSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIFND 775 GS+ K P A GSGS +A ++ +M +EE + +++A+A + D Sbjct: 142 GSLHKQPLAIGGSGSAFIYGFCDANFRENMTQEEAVEFLKNAVALAMERD 191 >SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 74.1 bits (174), Expect = 2e-14 Identities = 51/200 (25%), Positives = 94/200 (47%), Gaps = 3/200 (1%) Frame = +2 Query: 185 IPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDK 364 +PVP S + RN K GTT + Y G+++ D+RA+ +++ + Sbjct: 33 VPVPQSSL--YLRNLTDETKNKHCLIKMNHGTTTLAFRYQHGIVVCVDSRASAGPLIASQ 90 Query: 365 NCQKIHYLASNMYCCG--AGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRML 538 +K+ + N Y G AG AAD + + + L +L + V A+ +L + Sbjct: 91 TVKKV--IEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELISVSAASKILSNIT 148 Query: 539 FRYQGH-IGAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 715 + Y+G+ + +L G + G +Y I G+ K ++GSGS A V ++G++ D+ Sbjct: 149 YSYKGYGLSMGTMLAGTGKGGTALYYIDSDGTRLKGDLFSVGSGSTFAYGVLDSGYRWDL 208 Query: 716 NEEEGKKLVRDAIAAGIFND 775 +++E L + +I A D Sbjct: 209 SKQEALYLAQRSIVAATHRD 228 >SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 61.7 bits (143), Expect = 1e-10 Identities = 43/174 (24%), Positives = 79/174 (45%), Gaps = 11/174 (6%) Frame = +2 Query: 254 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 433 A +A K G+T +G+ D V+LG + R T + ++ + +K+ + S++ C +G AD Sbjct: 27 AIEAIKLGSTAIGVKTKDAVVLGVEKRLT-SPLMESHSVEKLFEIDSHIGCAISGLTADA 85 Query: 434 EMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRY-QGH----------IGAALVLG 580 + Q + R +E+ + + R+ +G G AL++ Sbjct: 86 RTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVALLIA 145 Query: 581 GVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLV 742 G+D GP +Y P G+ + +GSGS A + + +DM EE + L+ Sbjct: 146 GIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDMTLEEAEVLI 199 >SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 53.2 bits (122), Expect = 5e-08 Identities = 37/158 (23%), Positives = 73/158 (46%), Gaps = 7/158 (4%) Frame = +2 Query: 287 VGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQL 466 +GI+ DG++L A+ + T + +++ +K++++ NM C AG AD + + A ++ Sbjct: 35 LGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILI-NYARRV 93 Query: 467 ELQRLHT-GRTVPVETAATL---LKRMLFRYQG--HIGAALVLGGVDR-TGPHIYCIYPH 625 Q L T +P E LK+ +Y G G + + G D G ++ P Sbjct: 94 GQQYLQTFNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPS 153 Query: 626 GSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKL 739 G+ ++G S + ++ +K D+N +E + Sbjct: 154 GNYGSWQANSIGGNSTSVQSLMRQEYKDDINLDEASAM 191 >SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 245 Score = 45.6 bits (103), Expect = 9e-06 Identities = 46/193 (23%), Positives = 78/193 (40%), Gaps = 6/193 (3%) Frame = +2 Query: 200 FSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKI 379 FS F N L Q + A A G T VGI DGV+L + + T + + +K+ Sbjct: 7 FSLTTFSPNGKLVQIEY-ALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIG-ASLEKV 64 Query: 380 HYLASNMYCCGAGTAADTEMTTQSV--ASQLELQRLHTG----RTVPVETAATLLKRMLF 541 + ++ +G D + +Q ++++ + + E A+ + + Sbjct: 65 CAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASVMQESTQS 124 Query: 542 RYQGHIGAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNE 721 G +L++ G+D GP +Y + P G+ +G S AA E KR +E Sbjct: 125 GGVRPFGVSLLVAGMDEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLE---KRYNDE 181 Query: 722 EEGKKLVRDAIAA 760 E V AI A Sbjct: 182 LELDDAVHTAILA 194 >SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosaccharomyces pombe|chr 1|||Manual Length = 225 Score = 38.7 bits (86), Expect = 0.001 Identities = 29/132 (21%), Positives = 61/132 (46%), Gaps = 4/132 (3%) Frame = +2 Query: 257 PKATKTGTTIVGIIYADG-VILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 433 P GTT+ I DG IL DTR+ ++ + ++H + ++ +G AD Sbjct: 8 PYVQNGGTTVA--IAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADA 65 Query: 434 EMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLF--RYQGHIGAALVLGGVDRTGP-H 604 + + +++L + R + ++ A +++ +L+ R+ + + G+D+ G Sbjct: 66 LALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYY-VYTTVAGIDKEGKGE 124 Query: 605 IYCIYPHGSVDK 640 IY P GS ++ Sbjct: 125 IYSFDPVGSYER 136 >SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharomyces pombe|chr 3|||Manual Length = 253 Score = 33.9 bits (74), Expect = 0.031 Identities = 35/147 (23%), Positives = 54/147 (36%), Gaps = 5/147 (3%) Frame = +2 Query: 254 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 433 A KA + +T +GI DGVIL + T + N +I + ++ G D Sbjct: 26 AYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVN-NRIGSVDRHIGIATTGFIPDG 84 Query: 434 EMTTQSVASQLELQRLHTGRTVPVETAATLLKR--MLFRYQGHI---GAALVLGGVDRTG 598 + + + R + G +P A L LF + G + D G Sbjct: 85 QHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDSEG 144 Query: 599 PHIYCIYPHGSVDKLPYATMGSGSLAA 679 PH+Y + P+G A G G A Sbjct: 145 PHLYMVEPNGVYWGYNGAAAGKGRQVA 171 >SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 33.1 bits (72), Expect = 0.053 Identities = 35/152 (23%), Positives = 62/152 (40%), Gaps = 5/152 (3%) Frame = +2 Query: 254 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 433 A +A K G+ VG++ +L A R E K K+ + ++ AG A D Sbjct: 25 ALEAIKQGSATVGLVSKTHAVLVALKRNAEELSSYQK---KLIRIDDHIGIAIAGLAPDA 81 Query: 434 EMTTQSVASQLELQRLHTGRTVPVETAAT-LLKRMLFRYQGH----IGAALVLGGVDRTG 598 + + + + + R +PV + + ++ Q + G ++ G D +G Sbjct: 82 RVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQEYGRRPYGVGFLVIGYDESG 141 Query: 599 PHIYCIYPHGSVDKLPYATMGSGSLAAMAVFE 694 PH+ P G V + +MGS S +A E Sbjct: 142 PHLLEFQPSGLVLEYLGTSMGSRSQSARTYIE 173 >SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 31.9 bits (69), Expect = 0.12 Identities = 16/73 (21%), Positives = 39/73 (53%) Frame = +2 Query: 272 TGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQS 451 TG++++ + +ADGV++ AD A+ ++ + +++ + N G +D + Q Sbjct: 42 TGSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQ-IQR 100 Query: 452 VASQLELQRLHTG 490 + +LE++ + G Sbjct: 101 LLEKLEIKEGNYG 113 >SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 27.9 bits (59), Expect = 2.0 Identities = 12/33 (36%), Positives = 21/33 (63%) Frame = -1 Query: 631 RSMRINAVNMRTGSVNTSQNQSSPYMTLIAKQH 533 +S+ + M+TGSVN +Q+Q M ++A Q+ Sbjct: 267 QSIPSGPMGMKTGSVNDTQSQQITLMNVLASQN 299 >SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 995 Score = 27.1 bits (57), Expect = 3.5 Identities = 11/37 (29%), Positives = 20/37 (54%) Frame = -1 Query: 616 NAVNMRTGSVNTSQNQSSPYMTLIAKQHSFQQCGSSF 506 N + M+ S+ T + + +PY L+A + CGS + Sbjct: 861 NIIPMQKPSLTTHETKQNPYDLLVALDSDRKACGSLY 897 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,186,232 Number of Sequences: 5004 Number of extensions: 66706 Number of successful extensions: 176 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 168 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 172 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 438479610 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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