BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P30_F_C16
(562 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 136 2e-33
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 62 7e-11
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 59 4e-10
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 57 2e-09
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 42 6e-05
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 37 0.002
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 36 0.005
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 28 0.82
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.1
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 1.9
SPAC2E1P5.02c |mug109||sequence orphan|Schizosaccharomyces pombe... 26 4.4
SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|... 25 5.8
SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 7.6
SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 25 7.6
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 136 bits (330), Expect = 2e-33
Identities = 61/69 (88%), Positives = 67/69 (97%)
Frame = +1
Query: 34 PAIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESG 213
P IADRMQKEI ALAPS++K+KI+APPERKYSVWIGGSILASLSTFQQMWISK+EYDESG
Sbjct: 307 PGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 366
Query: 214 PGIVHRKCF 240
PGIV+RKCF
Sbjct: 367 PGIVYRKCF 375
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 61.7 bits (143), Expect = 7e-11
Identities = 30/69 (43%), Positives = 47/69 (68%), Gaps = 6/69 (8%)
Frame = +1
Query: 49 RMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGP- 216
R+Q E++ L P + ++KI A ER Y+ W+GGSIL+SL TF Q+WIS++EY+E G
Sbjct: 365 RLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSD 423
Query: 217 --GIVHRKC 237
++ ++C
Sbjct: 424 RLALIEKRC 432
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 59.3 bits (137), Expect = 4e-10
Identities = 27/65 (41%), Positives = 45/65 (69%)
Frame = +1
Query: 46 DRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIV 225
+R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ EEY ++ ++
Sbjct: 316 ERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVI 374
Query: 226 HRKCF 240
R+ F
Sbjct: 375 FRRRF 379
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 57.2 bits (132), Expect = 2e-09
Identities = 28/62 (45%), Positives = 38/62 (61%), Gaps = 2/62 (3%)
Frame = +1
Query: 34 PAIADRMQKEITALAP-STIKIKIIAPPER-KYSVWIGGSILASLSTFQQMWISKEEYDE 207
P +++R+Q E+ LA S I + +VW GGSILASL FQ +W+SK+EYDE
Sbjct: 357 PGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVSKQEYDE 416
Query: 208 SG 213
G
Sbjct: 417 VG 418
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 41.9 bits (94), Expect = 6e-05
Identities = 18/42 (42%), Positives = 30/42 (71%), Gaps = 1/42 (2%)
Frame = +1
Query: 91 KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKEEYDESG 213
K+KI P R+++V+IGG++LA ++ MW+SK E++E G
Sbjct: 337 KVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 36.7 bits (81), Expect = 0.002
Identities = 15/48 (31%), Positives = 26/48 (54%)
Frame = +1
Query: 88 IKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHR 231
+ + +I+ ++ +VW GGS+LA F +K +Y+E G I R
Sbjct: 372 VDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 35.5 bits (78), Expect = 0.005
Identities = 16/61 (26%), Positives = 29/61 (47%)
Frame = +1
Query: 34 PAIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESG 213
P + R+++E+T++ P I + W G S + F+ +++EEY E G
Sbjct: 648 PGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKG 707
Query: 214 P 216
P
Sbjct: 708 P 708
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 28.3 bits (60), Expect = 0.82
Identities = 14/70 (20%), Positives = 30/70 (42%), Gaps = 3/70 (4%)
Frame = +1
Query: 34 PAIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILASLSTFQQMWISKEEYD 204
P A +++ I + + I +I PP + ++ W G I + ++WI ++
Sbjct: 543 PGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNRIRIVSELWIKNSDWK 602
Query: 205 ESGPGIVHRK 234
G ++ K
Sbjct: 603 MLGSRVLQYK 612
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 27.9 bits (59), Expect = 1.1
Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%)
Frame = +1
Query: 133 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 234
++GGSI+A S + + +++ EEY + GP +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 27.1 bits (57), Expect = 1.9
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +1
Query: 58 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 168
KEI++ A S I + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
>SPAC2E1P5.02c |mug109||sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 163
Score = 25.8 bits (54), Expect = 4.4
Identities = 11/46 (23%), Positives = 24/46 (52%)
Frame = -3
Query: 167 VDREARMDPPIHTEYFLSGGAMILILMVEGARAVISFCILSAIAGV 30
+++ R+DP + ++++ + R SFCI+S+ AG+
Sbjct: 104 INQNIRLDPKYSIPGVCAVTLIVIMFLAPVGRLFWSFCIVSSFAGL 149
>SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 157
Score = 25.4 bits (53), Expect = 5.8
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -3
Query: 296 ELGRAQHHTAPGSRGRAA*KHLRWT 222
E+ R++ H+ PGS+G A +L WT
Sbjct: 46 EISRSETHSIPGSKGEA---NLIWT 67
>SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 879
Score = 25.0 bits (52), Expect = 7.6
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3
Query: 84 DHQDQDHRSPREEVLRMDRWIHPG 155
D D+ R+ E VLRM R++ PG
Sbjct: 499 DIDDKKDRALSEHVLRMHRYLPPG 522
>SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 981
Score = 25.0 bits (52), Expect = 7.6
Identities = 19/58 (32%), Positives = 25/58 (43%), Gaps = 2/58 (3%)
Frame = +2
Query: 287 VRVLNATVSTLYLVIP--EN*TSNLTPSILM*FIVKFYINLISFLFLLYTRDRCGSQR 454
VR +A S +L P N SN TP+ + I K N+ S + L D C R
Sbjct: 575 VRFFHAIQSHFHLEGPIRYNMNSNCTPNSIASIIQKKSYNVSSITYELLNEDACALSR 632
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,729,191
Number of Sequences: 5004
Number of extensions: 30569
Number of successful extensions: 71
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 68
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 70
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 236012634
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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