Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P29_pT_O05
(678 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 75 7e-15
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 70 4e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 63 4e-11
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 43 4e-05
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 42 8e-05
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 1.9
SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc... 27 2.5
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 4.4
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 75.4 bits (177), Expect = 7e-15
Identities = 52/144 (36%), Positives = 77/144 (53%), Gaps = 3/144 (2%)
Frame = -3
Query: 523 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 350
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 349 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTTRREA****GR 170
F FL + K + D + E + FDKDGNG+I+ EL H+L T+ E
Sbjct: 67 FTEFLTM--MARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVL-TSLGERLSQEEV 123
Query: 169 ATPXRDKKT-LQGNINYENFVHLI 101
A R+ T G INYE F +I
Sbjct: 124 ADMIREADTDGDGVINYEEFSRVI 147
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 69.7 bits (163), Expect = 4e-13
Identities = 45/135 (33%), Positives = 68/135 (50%)
Frame = -3
Query: 502 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQ 323
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 322 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTTRREA****GRATPXRDKKT 143
+ +F++G + FDKD G I ELR++L T+ E +
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVL-TSLGEKLSNEEMDELLKGVPV 124
Query: 142 LQGNINYENFVHLIM 98
G +NY +FV +I+
Sbjct: 125 KDGMVNYHDFVQMIL 139
Score = 27.5 bits (58), Expect = 1.9
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = -3
Query: 505 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 392
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 62.9 bits (146), Expect = 4e-11
Identities = 41/142 (28%), Positives = 72/142 (50%)
Frame = -3
Query: 523 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFE 344
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 343 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTTRREA****GRAT 164
F+ +K R ++ ++I+ R FDKD +G+I +A+ + T + +
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLM 119
Query: 163 PXRDKKTLQGNINYENFVHLIM 98
T G+ +Y +FV IM
Sbjct: 120 VQEADPTNSGSFDYYDFVQRIM 141
Score = 35.5 bits (78), Expect = 0.007
Identities = 16/65 (24%), Positives = 35/65 (53%)
Frame = -3
Query: 505 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 326
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 325 QAISK 311
+ ++K
Sbjct: 139 RIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 43.2 bits (97), Expect = 4e-05
Identities = 32/106 (30%), Positives = 47/106 (44%), Gaps = 2/106 (1%)
Frame = -3
Query: 523 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 350
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K + +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 349 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHL 212
E F+ + K D + FD D G IS LR +
Sbjct: 92 MEDFVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRV 135
Score = 30.3 bits (65), Expect = 0.27
Identities = 18/65 (27%), Positives = 31/65 (47%), Gaps = 2/65 (3%)
Frame = -3
Query: 517 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKC--TLHLKPDERISFE 344
D L E + AF+LFD GKI + + + L +N + +++ L D I+ +
Sbjct: 107 DPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQ 166
Query: 343 VFLPI 329
F+ I
Sbjct: 167 EFIAI 171
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 41.9 bits (94), Expect = 8e-05
Identities = 31/110 (28%), Positives = 48/110 (43%)
Frame = -3
Query: 532 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERI 353
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 352 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLST 203
+ FL ++ S ND +E FD +G I + +R LS+
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSS 145
Score = 29.5 bits (63), Expect = 0.47
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = -3
Query: 496 EAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 389
EAF FD GKI ++ + DAL ++G +V+
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVE 156
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.5 bits (58), Expect = 1.9
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = -3
Query: 505 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 326
E +EAF LFD G I + + LG+N T+ ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 325 QAIS 314
IS
Sbjct: 73 IHIS 76
>SPBC23E6.07c |rfc1||DNA replication factor C complex subunit
Rfc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 934
Score = 27.1 bits (57), Expect = 2.5
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = -1
Query: 198 GEKLSDDEVEQLLXGTRRLYREISTMRTLFTSSC 97
G KLS V+QL+ GT+ R+I + + + SC
Sbjct: 558 GLKLSPQAVDQLVQGTQSDMRQIINLLSTYKLSC 591
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 4.4
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = +1
Query: 424 LLTHLRSERHVSFHLHEN--RRAGRLLEILPIDPLNIQPYYESTNEIEGNEIGIFKGCDL 597
+ TH+ SE L+E R ++ +LP+ P +++P G + K D+
Sbjct: 216 IFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDI 275
Query: 598 SKSN 609
K+N
Sbjct: 276 IKAN 279
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,534,540
Number of Sequences: 5004
Number of extensions: 49604
Number of successful extensions: 167
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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