Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P29_pT_H18
(820 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 132 8e-32
SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac... 115 7e-27
SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi... 81 2e-16
SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 75 9e-15
SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 73 7e-14
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 29 1.0
SPAC1296.03c |sxa2||serine carboxypeptidase Sxa2|Schizosaccharom... 28 1.8
SPBC2G2.07c |mug178||mitochondrial ribosomal protein subunit L51... 28 1.8
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 26 7.4
SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|... 26 7.4
SPBC543.05c |||inorganic anion exchanger |Schizosaccharomyces po... 25 9.8
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 25 9.8
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 25 9.8
>SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 334
Score = 132 bits (318), Expect = 8e-32
Identities = 76/242 (31%), Positives = 121/242 (50%)
Frame = -2
Query: 819 IXVGHDHIDVAECKKRGVRIGYTPDVXXXXXXXXXXXXXXXTSRRVPEAIHEAKTGGWVS 640
+ G++ +DVA C RG+++ + P R + I E W +
Sbjct: 87 LGAGYETVDVAACTARGIQVSHVPKAVDDATADVGIFLMLGALRGFNQGIFELHKNNWNA 146
Query: 639 WAPTWMTGPGLAGATVGIVGFGRIGQAVARRVKAFNTERIIYFNRSHRPEEKETGAVKVS 460
G T+GI+G G IG+ +A+R +AF+ +I+Y NR+ PEE+ GA VS
Sbjct: 147 ---NCKPSHDPEGKTLGILGLGGIGKTMAKRARAFDM-KIVYHNRTPLPEEEAEGAEFVS 202
Query: 459 FDELLTQSDFVICCAALVPETKEIFNKEAFEKMKDTAIFVNTSRGGTVDQDALIEALKTN 280
FD+LL +SD + L T+ I K F+KMK + VNT+RG +D+ AL+EAL
Sbjct: 203 FDDLLAKSDVLSLNLPLNAHTRHIIGKPEFQKMKRGIVIVNTARGAVMDEAALVEALDEG 262
Query: 279 KIRAAGLDVTSPEPLPLDNPLFKLSNCVVLPHIGSATIEARNTMSELTARNILAALHGTD 100
+ +AGLDV EP + L + ++LPH+G+ ++E + M N+ +
Sbjct: 263 IVYSAGLDVFEEEP-KIHPGLLENEKVILLPHLGTNSLETQYKMECAVLMNVKNGIVNDS 321
Query: 99 MP 94
+P
Sbjct: 322 LP 323
>SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 340
Score = 115 bits (277), Expect = 7e-27
Identities = 72/216 (33%), Positives = 106/216 (49%), Gaps = 2/216 (0%)
Frame = -2
Query: 810 GHDHIDVAECKKRGVRIGYTPDVXXXXXXXXXXXXXXXTSRRVPEAIHEAKTGGWVSWAP 631
G++++DV + GV + TP+ T R EA + G W
Sbjct: 93 GYNNVDVDWATRNGVYVANTPNGPTEGTANMNLMLFMCTLRGAREAEQSLRLG---KWRQ 149
Query: 630 TWMTGPGLAGATVGIVGFGRIGQAVARRVKAFNTERIIYFNRSH--RPEEKETGAVKVSF 457
G VGI+G G IG++ A+++ E I+Y NR+ EEK GA VSF
Sbjct: 150 NLSLTDDPYGKRVGIIGMGAIGKSFAQKILPLGCE-IVYHNRNRLEAEEEKRLGASFVSF 208
Query: 456 DELLTQSDFVICCAALVPETKEIFNKEAFEKMKDTAIFVNTSRGGTVDQDALIEALKTNK 277
DELL+ SD + L P T ++ + + FEKMKD +NT+RG +++DA I+A+K+ K
Sbjct: 209 DELLSSSDVISINCPLTPATHDLISTKEFEKMKDGVYIINTARGAIINEDAFIKAIKSGK 268
Query: 276 IRAAGLDVTSPEPLPLDNPLFKLSNCVVLPHIGSAT 169
+ AGLDV EP P + + + PH G T
Sbjct: 269 VARAGLDVFLNEPTP-NKFWLECDKVTIQPHCGVYT 303
>SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 466
Score = 81.0 bits (191), Expect = 2e-16
Identities = 65/234 (27%), Positives = 107/234 (45%), Gaps = 15/234 (6%)
Frame = -2
Query: 813 VGHDHIDVAECKKRGVRIGYTPDVXXXXXXXXXXXXXXXTSRRVPEAIHEAKTGGWVSWA 634
+G + +D+ +RG+ + +P +R+V + E G W +
Sbjct: 128 IGTNQVDLDFAAERGIAVFNSPYANSRSVAELVIGYIISLARQVGDRSLELHRGEWNKVS 187
Query: 633 P-TWMTGPGLAGATVGIVGFGRIGQAVARRVKAFNTERIIYFNRSHRPEEKETGAVKV-- 463
W + G T+GI+G+G IG ++ +A + Y P G+ K
Sbjct: 188 SGCWE----IRGKTLGIIGYGHIGSQLSVLAEAMGLHVVYYDILPIMP----LGSAKQLS 239
Query: 462 SFDELLTQSDFVICCAALVPETKEIFNKEAFEKMKDTAIFVNTSRGGTVDQDALIEALKT 283
S ELL ++DFV PETK + + + F MK+ + +N SRG VD AL++A K+
Sbjct: 240 SLPELLHRADFVSLHVPASPETKNMISSKEFAAMKEGSYLINASRGTVVDIPALVDASKS 299
Query: 282 NKIRAAGLDVTSPEPL---------PLDNPLFKLSNC---VVLPHIGSATIEAR 157
KI A +DV EP L++ +L++C ++ PHIG +T EA+
Sbjct: 300 GKIAGAAIDVYPSEPAGNGKDKFVDSLNSWTSELTHCKNIILTPHIGGSTEEAQ 353
>SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 332
Score = 75.4 bits (177), Expect = 9e-15
Identities = 53/181 (29%), Positives = 90/181 (49%), Gaps = 14/181 (7%)
Frame = -2
Query: 624 MTGPGLAGATVGIVGFGRIGQAVARRVK-AFNTERIIYFNRSHRPEEKETGAVKVSFDEL 448
+ G L G T+G++G GRIG VA+ +K F E + + + ++ EK G V E+
Sbjct: 137 LLGHDLHGKTIGLLGTGRIGGLVAKCLKLGFGCEVLAHDIKPNKELEK-FGIQFVEQQEV 195
Query: 447 LTQSDFVICCAALVPETKEIFNKEAFEKMKDTAIFVNTSRGGTVDQDALIEALKTNKIRA 268
L ++DF+ L P+T+ + +++ MK +NTSRGG VD AL++A+++ ++
Sbjct: 196 LAKADFLCLHCPLTPDTEHLVDEKLLASMKKGVKIINTSRGGLVDTKALVKAIESGQVGG 255
Query: 267 AGLDVTSPEP----LPLDNPLFK---------LSNCVVLPHIGSATIEARNTMSELTARN 127
+DV E L N + K N +V H T EA + ++ T +N
Sbjct: 256 CAMDVYEGERRLFYRDLSNEVIKDTTFQQLANFPNVLVTSHQAFFTAEALSAIAHTTLKN 315
Query: 126 I 124
+
Sbjct: 316 V 316
>SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 332
Score = 72.5 bits (170), Expect = 7e-14
Identities = 42/129 (32%), Positives = 66/129 (51%), Gaps = 1/129 (0%)
Frame = -2
Query: 624 MTGPGLAGATVGIVGFGRIGQAVARRVK-AFNTERIIYFNRSHRPEEKETGAVKVSFDEL 448
+ G + G TVG++G G+IG VA+ K F + + Y + E G V +E+
Sbjct: 137 LLGCDIHGKTVGVIGTGKIGSNVAKCFKMGFGCDVLAYDINPDKKLENY-GVQFVEQNEV 195
Query: 447 LTQSDFVICCAALVPETKEIFNKEAFEKMKDTAIFVNTSRGGTVDQDALIEALKTNKIRA 268
L ++DF+ L P T I N ++ MK VNTSRGG +D AL++A+ + ++
Sbjct: 196 LKKADFLCLHCPLTPSTTHIVNSDSLALMKKGVTIVNTSRGGLIDTKALVDAIDSGQVGG 255
Query: 267 AGLDVTSPE 241
+DV E
Sbjct: 256 CAIDVYEGE 264
>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 336
Score = 28.7 bits (61), Expect = 1.0
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -2
Query: 594 VGIVGFGRIGQAVARRVKAFNTERIIYFN 508
VGI GFGRIG+ V R T +++ N
Sbjct: 6 VGINGFGRIGRIVLRNALVAKTIQVVAIN 34
>SPAC1296.03c |sxa2||serine carboxypeptidase
Sxa2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 27.9 bits (59), Expect = 1.8
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 4/35 (11%)
Frame = +2
Query: 371 NASLLNISLVSGTSA----AQQITKSLWVRSSSKL 463
N + + + +VSG +A +QIT S+WV SKL
Sbjct: 220 NINFMGVGIVSGLTADYETQEQITASIWVEHISKL 254
>SPBC2G2.07c |mug178||mitochondrial ribosomal protein subunit
L51-b|Schizosaccharomyces pombe|chr 2|||Manual
Length = 225
Score = 27.9 bits (59), Expect = 1.8
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +2
Query: 287 FSASIKAS*STVPPRLVFTKMAVSFIFSNASLLNISLVS 403
FS+S S T+PP +++ K+ + FS++ +SLVS
Sbjct: 117 FSSSNPKSRPTLPPGILYAKLKLLDSFSDSEKKRLSLVS 155
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1428
Score = 25.8 bits (54), Expect = 7.4
Identities = 9/30 (30%), Positives = 19/30 (63%)
Frame = +1
Query: 298 NQSILIYSSTTTCIYKNGCILHLFKRFLVE 387
N+ L+Y S T+ +Y CI+H ++++ +
Sbjct: 1177 NRQPLLYGSGTSTLYNEICIVHPEEKYICQ 1206
>SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr
1|||Manual
Length = 809
Score = 25.8 bits (54), Expect = 7.4
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +1
Query: 436 ALGEEFIETDFDSTSFLLFRSMTAIKINDSFCVECFNPSCYCLTYS 573
AL + + F + FR++ AI S+C+E N S + + YS
Sbjct: 642 ALESSRLSSLFKEFDLVSFRTVPAIHCPYSYCMEITNSSGWKIAYS 687
>SPBC543.05c |||inorganic anion exchanger |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 517
Score = 25.4 bits (53), Expect = 9.8
Identities = 11/34 (32%), Positives = 21/34 (61%), Gaps = 3/34 (8%)
Frame = -3
Query: 263 VWML---RLLNHYLWIIHCSS*AIVWFYLI*EVL 171
+WML ++++ + HCSS +VW Y I +++
Sbjct: 427 IWMLSDRKVVSKNHTLNHCSSKRVVWLYTILQLI 460
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 25.4 bits (53), Expect = 9.8
Identities = 12/46 (26%), Positives = 28/46 (60%)
Frame = -3
Query: 485 RKLVLSKSVSMNSSPKATL*FVVQH*FLKLKRYSTRKRLKR*RIQP 348
RK+VLS +++ S + + +V+ K+K+++++ L+ +QP
Sbjct: 364 RKVVLSTNIAETSVTISGIRYVIDTGLAKIKQFNSKLGLESLTVQP 409
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 25.4 bits (53), Expect = 9.8
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -2
Query: 606 AGATVGIVGFGRIGQAVARRVKAFNTERII 517
+G+TV +VG G +G A + A RII
Sbjct: 193 SGSTVAVVGCGCVGLAAMQGAVAAGASRII 222
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,275,593
Number of Sequences: 5004
Number of extensions: 63901
Number of successful extensions: 198
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 400438000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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