Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P29_pT_F22
(787 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 282 3e-77
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 282 3e-77
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 282 3e-77
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 86 5e-18
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 49 7e-07
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 36 0.007
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.0
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 7.0
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.3
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 282 bits (692), Expect = 3e-77
Identities = 128/169 (75%), Positives = 146/169 (86%)
Frame = -3
Query: 785 KPGTIVVFAPANIXTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNN 606
KPG IV FAPA + TEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+
Sbjct: 271 KPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKND 330
Query: 605 PPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPK 426
PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK
Sbjct: 331 PPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPK 390
Query: 425 SIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 279
+KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 391 FVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 282 bits (692), Expect = 3e-77
Identities = 128/169 (75%), Positives = 146/169 (86%)
Frame = -3
Query: 785 KPGTIVVFAPANIXTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNN 606
KPG IV FAPA + TEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+
Sbjct: 271 KPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKND 330
Query: 605 PPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPK 426
PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK
Sbjct: 331 PPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPK 390
Query: 425 SIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 279
+KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 391 FVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 282 bits (692), Expect = 3e-77
Identities = 128/169 (75%), Positives = 146/169 (86%)
Frame = -3
Query: 785 KPGTIVVFAPANIXTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNN 606
KPG IV FAPA + TEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+
Sbjct: 271 KPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKND 330
Query: 605 PPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPK 426
PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK
Sbjct: 331 PPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPK 390
Query: 425 SIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 279
+KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 391 FVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 86.2 bits (204), Expect = 5e-18
Identities = 53/172 (30%), Positives = 89/172 (51%), Gaps = 2/172 (1%)
Frame = -3
Query: 785 KPGTIVVFAPANIXTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKN 609
K + V+ P N EV ++ + E + ++ GD V V+ +++ GYV +KN
Sbjct: 494 KKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN 552
Query: 608 NPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNP 429
P F AQ+ +L P ++ GY+ V+ HTA FA++ K+D+ T + ++ P
Sbjct: 553 -PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPP 610
Query: 428 KSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 276
G I L P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 611 MFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 49.2 bits (112), Expect = 7e-07
Identities = 41/138 (29%), Positives = 63/138 (45%)
Frame = -3
Query: 698 AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVL 519
AV GD V + ++ V +LR G + + +N P + F A++ + G I +G T VL
Sbjct: 461 AVAGDTVTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL 519
Query: 518 DCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 339
H+ + K+ K + + S K I L PLC+ +E P L
Sbjct: 520 -----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPAL 571
Query: 338 GRFAVRDMRQTVAVGVIK 285
GRF +R TVA G++K
Sbjct: 572 GRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 35.9 bits (79), Expect = 0.007
Identities = 33/156 (21%), Positives = 65/156 (41%)
Frame = -3
Query: 755 ANIXTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTA 576
+++ T V +EM + L AV GDN G ++++ ++L+RG + P F A
Sbjct: 292 SHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKA 349
Query: 575 QVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIV 396
+L + T +D + + + +++ ++ T + K + GD +
Sbjct: 350 SFYILTK--EEGGRRTGFVDKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEM 404
Query: 395 NLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 288
P+ +E Q RF VR+ TV ++
Sbjct: 405 ICTLIHPIVIEKGQ------RFTVREGGSTVGTALV 434
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +3
Query: 642 QFLDGHVLYVETYIVSRYSFLESFV 716
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 7.0
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +1
Query: 235 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 408
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 409 SPDLMDFGLTSVDLPVRRSTFSLIS 483
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 9.3
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +1
Query: 400 MAASPDLMDFGLTSVDLPVRRSTFS 474
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,171,461
Number of Sequences: 5004
Number of extensions: 65274
Number of successful extensions: 177
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 381366860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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