BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P29_pT_E23
(389 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schi... 76 2e-15
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 27 1.4
SPAC27E2.04c |mug155||sequence orphan|Schizosaccharomyces pombe|... 25 5.5
SPBC428.03c |pho4||thiamine-repressible acid phosphatase Pho4|Sc... 25 5.5
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 24 7.2
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 24 7.2
SPCC1919.11 |mug137||BAR adaptor protein|Schizosaccharomyces pom... 24 7.2
SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosacch... 24 9.5
>SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier
Hub1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 73
Score = 76.2 bits (179), Expect = 2e-15
Identities = 38/73 (52%), Positives = 50/73 (68%)
Frame = -2
Query: 226 MLEVTCNDRLGKKVRVKCNPDDXRG*FKEVNSRTNWH*IRQNSTQEMVHSIXRIILNLRD 47
M+EV CNDRLGKKVRVKC PDD G FK++ + R+ ++ HS+ + + L D
Sbjct: 1 MIEVLCNDRLGKKVRVKCMPDDTVGDFKKLVAAQTGTDPRRIVLKKW-HSVFKDNITLAD 59
Query: 46 YEIHDGMNLELYY 8
YEIHDGM+LE+YY
Sbjct: 60 YEIHDGMSLEMYY 72
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 26.6 bits (56), Expect = 1.4
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2
Query: 131 AINFFKSPTXIVRVAFNSNLLTESIIACYFKHFA 232
A+ + P I RV +SNLL + YF F+
Sbjct: 623 ALELYDKPADIKRVIVHSNLLNPEWLMNYFSRFS 656
>SPAC27E2.04c |mug155||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 187
Score = 24.6 bits (51), Expect = 5.5
Identities = 15/52 (28%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
Frame = +1
Query: 208 CMLLQAFCSQISSQFY*EIKYINSNFLFVLVILPPFKIFTAVL--MVFITFF 357
C L + F +S + +I+ + + VL++LPPF ++ VF T F
Sbjct: 69 CRLKKKFIKSLSKKII-SYHFISFHTIVVLLLLPPFSHLLVLVYPSVFTTAF 119
>SPBC428.03c |pho4||thiamine-repressible acid phosphatase
Pho4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 463
Score = 24.6 bits (51), Expect = 5.5
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +2
Query: 107 SYLVPVCAAINFFKSPTXIVRVAFNSNLLTESIIACYFKHFA 232
S ++PV AA+ FF T + + N+ T S+ F FA
Sbjct: 342 SQIIPVEAALGFFPDITPEHPLPTDKNIFTYSLKTSSFVPFA 383
>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 606
Score = 24.2 bits (50), Expect = 7.2
Identities = 12/37 (32%), Positives = 16/37 (43%)
Frame = +1
Query: 268 YINSNFLFVLVILPPFKIFTAVLMVFITFFSLNHRPA 378
Y N N FV VIL F V+ +F + P+
Sbjct: 343 YSNKNVYFVTVILSSMSKFAEFNSVYNKYFDFTNPPS 379
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 24.2 bits (50), Expect = 7.2
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 238 ISSQFY*EIKYINSNFLFVLVILPPFKIFT 327
ISS Y ++ + +SNFLF + + + + T
Sbjct: 1204 ISSTLYLKVSFCSSNFLFKTISVLVYDLIT 1233
>SPCC1919.11 |mug137||BAR adaptor protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 420
Score = 24.2 bits (50), Expect = 7.2
Identities = 12/22 (54%), Positives = 14/22 (63%), Gaps = 1/22 (4%)
Frame = +2
Query: 134 INFFKSPTXIVRVAFNS-NLLT 196
+NFFK T I+ FNS N LT
Sbjct: 206 LNFFKESTGILNTIFNSQNSLT 227
>SPAP8A3.14c |||mitochondrial inner membrane protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 677
Score = 23.8 bits (49), Expect = 9.5
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = +1
Query: 1 IIDSTAPNSFHRGFHSLASLI*SLXYCVPFLEYYFVVSSASLCG 132
I+D T SF+R S SL SL Y P +E + + L G
Sbjct: 480 ILDETTRKSFYRIILSSNSLSTSLTY--PLIEIILPIKNGFLMG 521
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,374,678
Number of Sequences: 5004
Number of extensions: 22566
Number of successful extensions: 59
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 56
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 57
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 128029482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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