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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P29_pT_E23
         (389 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schi...    76   2e-15
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    27   1.4  
SPAC27E2.04c |mug155||sequence orphan|Schizosaccharomyces pombe|...    25   5.5  
SPBC428.03c |pho4||thiamine-repressible acid phosphatase Pho4|Sc...    25   5.5  
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c...    24   7.2  
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc...    24   7.2  
SPCC1919.11 |mug137||BAR adaptor protein|Schizosaccharomyces pom...    24   7.2  
SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosacch...    24   9.5  

>SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier
           Hub1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 73

 Score = 76.2 bits (179), Expect = 2e-15
 Identities = 38/73 (52%), Positives = 50/73 (68%)
 Frame = -2

Query: 226 MLEVTCNDRLGKKVRVKCNPDDXRG*FKEVNSRTNWH*IRQNSTQEMVHSIXRIILNLRD 47
           M+EV CNDRLGKKVRVKC PDD  G FK++ +       R+   ++  HS+ +  + L D
Sbjct: 1   MIEVLCNDRLGKKVRVKCMPDDTVGDFKKLVAAQTGTDPRRIVLKKW-HSVFKDNITLAD 59

Query: 46  YEIHDGMNLELYY 8
           YEIHDGM+LE+YY
Sbjct: 60  YEIHDGMSLEMYY 72


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1666

 Score = 26.6 bits (56), Expect = 1.4
 Identities = 12/34 (35%), Positives = 17/34 (50%)
 Frame = +2

Query: 131 AINFFKSPTXIVRVAFNSNLLTESIIACYFKHFA 232
           A+  +  P  I RV  +SNLL    +  YF  F+
Sbjct: 623 ALELYDKPADIKRVIVHSNLLNPEWLMNYFSRFS 656


>SPAC27E2.04c |mug155||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 187

 Score = 24.6 bits (51), Expect = 5.5
 Identities = 15/52 (28%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
 Frame = +1

Query: 208 CMLLQAFCSQISSQFY*EIKYINSNFLFVLVILPPFKIFTAVL--MVFITFF 357
           C L + F   +S +      +I+ + + VL++LPPF     ++   VF T F
Sbjct: 69  CRLKKKFIKSLSKKII-SYHFISFHTIVVLLLLPPFSHLLVLVYPSVFTTAF 119


>SPBC428.03c |pho4||thiamine-repressible acid phosphatase
           Pho4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 463

 Score = 24.6 bits (51), Expect = 5.5
 Identities = 14/42 (33%), Positives = 21/42 (50%)
 Frame = +2

Query: 107 SYLVPVCAAINFFKSPTXIVRVAFNSNLLTESIIACYFKHFA 232
           S ++PV AA+ FF   T    +  + N+ T S+    F  FA
Sbjct: 342 SQIIPVEAALGFFPDITPEHPLPTDKNIFTYSLKTSSFVPFA 383


>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 606

 Score = 24.2 bits (50), Expect = 7.2
 Identities = 12/37 (32%), Positives = 16/37 (43%)
 Frame = +1

Query: 268 YINSNFLFVLVILPPFKIFTAVLMVFITFFSLNHRPA 378
           Y N N  FV VIL     F     V+  +F   + P+
Sbjct: 343 YSNKNVYFVTVILSSMSKFAEFNSVYNKYFDFTNPPS 379


>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1233

 Score = 24.2 bits (50), Expect = 7.2
 Identities = 10/30 (33%), Positives = 18/30 (60%)
 Frame = +1

Query: 238  ISSQFY*EIKYINSNFLFVLVILPPFKIFT 327
            ISS  Y ++ + +SNFLF  + +  + + T
Sbjct: 1204 ISSTLYLKVSFCSSNFLFKTISVLVYDLIT 1233


>SPCC1919.11 |mug137||BAR adaptor protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 420

 Score = 24.2 bits (50), Expect = 7.2
 Identities = 12/22 (54%), Positives = 14/22 (63%), Gaps = 1/22 (4%)
 Frame = +2

Query: 134 INFFKSPTXIVRVAFNS-NLLT 196
           +NFFK  T I+   FNS N LT
Sbjct: 206 LNFFKESTGILNTIFNSQNSLT 227


>SPAP8A3.14c |||mitochondrial inner membrane protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 677

 Score = 23.8 bits (49), Expect = 9.5
 Identities = 16/44 (36%), Positives = 21/44 (47%)
 Frame = +1

Query: 1   IIDSTAPNSFHRGFHSLASLI*SLXYCVPFLEYYFVVSSASLCG 132
           I+D T   SF+R   S  SL  SL Y  P +E    + +  L G
Sbjct: 480 ILDETTRKSFYRIILSSNSLSTSLTY--PLIEIILPIKNGFLMG 521


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,374,678
Number of Sequences: 5004
Number of extensions: 22566
Number of successful extensions: 59
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 56
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 57
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 128029482
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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