Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P29_F_N19
(824 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharom... 65 1e-11
SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharom... 30 0.35
SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein At... 28 1.4
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 28 1.8
SPBC23G7.07c |||replication regulator |Schizosaccharomyces pombe... 26 5.6
SPBC776.06c |||spindle pole body interacting protein |Schizosacc... 26 7.5
SPCC1827.04 |||ankyrin repeat protein, unknown biological role|S... 25 9.9
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 25 9.9
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 25 9.9
>SPBC2D10.09 |||3-hydroxyisobutyryl-CoA
hydrolase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 429
Score = 64.9 bits (151), Expect = 1e-11
Identities = 40/184 (21%), Positives = 78/184 (42%), Gaps = 3/184 (1%)
Frame = +3
Query: 249 NEYITTRENNGTREITLNHEKTKNSLSLNMMNHLIEAINKNKEDISLRAIIISAKGNVFS 428
N+ + NG R TLN K N+++++M++ ++ + +E + II+ G FS
Sbjct: 55 NDTVLYESKNGARIFTLNRPKVLNAINVDMIDSILPKLVSLEESNLAKVIILKGNGRSFS 114
Query: 429 AGHNLK--ELQSSSG-VDQHKEIFSKATELMKSIILSPVPVIAKVNGFATAAGCQLVATC 599
+G ++K L G + + + F++ L ++ PV+A +NG G L
Sbjct: 115 SGGDIKAAALSIQDGKLPEVRHAFAQEYRLSHTLATYQKPVVALMNGITMGGGSGLAMHV 174
Query: 600 DIIVCSDSSKFSTPGANFGIFCSTPGIALGRSVCKSKATYMLFTGEPINAQEAYESGLVT 779
+ + + F+ P G F + TY+ T + + + +G+ T
Sbjct: 175 PFRIACEDTMFAMPETGIGYFTDVAASFFFSRLPGYFGTYLGLTSQIVKGYDCLRTGIAT 234
Query: 780 KVVP 791
VP
Sbjct: 235 HFVP 238
>SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 892
Score = 30.3 bits (65), Expect = 0.35
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = +3
Query: 195 FVQKTGSRSLSVLRRFVHNEYITTRENNGTREITLNHEKTKNSLSLNMM 341
F+Q + LR VH+++I +R N HE K + L +M
Sbjct: 405 FLQSLSQEEIDDLRAMVHSDHIYSRLTNSLAPSVYGHEIIKKGILLQLM 453
>SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein Atg13
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 758
Score = 28.3 bits (60), Expect = 1.4
Identities = 29/112 (25%), Positives = 49/112 (43%), Gaps = 5/112 (4%)
Frame = +3
Query: 378 DISLRAIIISAKGNVFSAGHNLKELQSSSGVDQHKEIFSKATELMK-----SIILSPVPV 542
D L ++SA + +A +N ++ + DQ F AT L K S++ S +
Sbjct: 271 DALLSNQLLSADKHQLAASNNSQDFEDGKQYDQPPPSF--ATRLAKQSDPNSLLQSEIQH 328
Query: 543 IAKVNGFATAAGCQLVATCDIIVCSDSSKFSTPGANFGIFCSTPGIALGRSV 698
+A + TA LV I S ++PG+NF +P +A+ R +
Sbjct: 329 LASIESI-TAQAAPLVT---IHPFKSPSLSASPGSNFDNMSISPKVAVNRYI 376
>SPBC2F12.13 |klp5|sot1|kinesin-like protein
Klp5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 883
Score = 27.9 bits (59), Expect = 1.8
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +3
Query: 528 SPVPVIAKVNGFATAAGCQLVATCDIIVCSDSSKFSTPGA 647
S + V +V F+TA L+A+ D + SS PG+
Sbjct: 5 SSITVTVRVRPFSTAESANLIASSDRLSFGTSSSLRNPGS 44
>SPBC23G7.07c |||replication regulator |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 277
Score = 26.2 bits (55), Expect = 5.6
Identities = 9/22 (40%), Positives = 15/22 (68%)
Frame = +2
Query: 185 HVQFCSEDRIKVIVGTTPFCSQ 250
H+ +C ++I V +GTTP +Q
Sbjct: 195 HINYCKANKIGVGIGTTPRIAQ 216
>SPBC776.06c |||spindle pole body interacting protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 598
Score = 25.8 bits (54), Expect = 7.5
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +3
Query: 348 LIEAINKNKEDISLRAIIISAKGNVFSAGHNLKEL 452
L + +++ +ED SL I S K NVFS N+ ++
Sbjct: 40 LPDRVHERREDWSLFFIHFSKKSNVFSLFSNVDDI 74
>SPCC1827.04 |||ankyrin repeat protein, unknown biological
role|Schizosaccharomyces pombe|chr 3|||Manual
Length = 600
Score = 25.4 bits (53), Expect = 9.9
Identities = 13/51 (25%), Positives = 25/51 (49%)
Frame = +3
Query: 405 SAKGNVFSAGHNLKELQSSSGVDQHKEIFSKATELMKSIILSPVPVIAKVN 557
+ KGN+ SAG L+ + + +++F +L+++ L V I N
Sbjct: 254 NTKGNIHSAGSGLRRYNEQALIKDIQQVFKDWGKLLETCDLIFVRAIGSSN 304
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 25.4 bits (53), Expect = 9.9
Identities = 13/54 (24%), Positives = 20/54 (37%)
Frame = -1
Query: 755 FLCIYWFTREKHISCFRFTNRSSKSNSRCGTEYTKVCTRSGKLA*VRTYDYITG 594
F C F CF+ T S C +Y +C + G + ++ D G
Sbjct: 172 FQCNVCFDEFNGTDCFQLTRCGHVSCQSCLRDYYTMCIQEGMFSQIKCIDLDCG 225
>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 25.4 bits (53), Expect = 9.9
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +2
Query: 254 IYNY*RK*WNKRNHTES*KDQEFTITEHDEPFDRSYQ*KQRGH 382
++N RK WN+R KD+ + T P S++ +RGH
Sbjct: 34 LFNAIRKLWNERRRYRMPKDESISPT----PISYSFRSLRRGH 72
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,120,925
Number of Sequences: 5004
Number of extensions: 62059
Number of successful extensions: 158
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 404442380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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