Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P29_F_J05
         (838 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    71   2e-13
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    71   2e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    57   3e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    32   0.12 
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    28   1.9  
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    27   3.3  
SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr...    26   5.8  
SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|...    26   5.8  
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    26   7.6  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 70.9 bits (166), Expect = 2e-13
 Identities = 47/151 (31%), Positives = 76/151 (50%), Gaps = 3/151 (1%)
 Frame = +3

Query: 102 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 275
           M+  +  D + A F  AFS++D +  G I +  LG ++R+L  +PT A +          
Sbjct: 1   MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60

Query: 276 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 452
                    FL + ++  KD D    E+  E  K++DK+ NG +   ELTH L +LGE+L
Sbjct: 61  GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118

Query: 453 DDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 545
              EVA++ ++     D DG+I Y  F + +
Sbjct: 119 SQEEVADMIREA--DTDGDGVINYEEFSRVI 147


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 70.9 bits (166), Expect = 2e-13
 Identities = 44/136 (32%), Positives = 71/136 (52%)
 Frame = +3

Query: 144 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 323
           AFS++D  G G+I   ++GDLLRA   NPTLA I                  FL + ++ 
Sbjct: 11  AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67

Query: 324 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 503
                 G  E+F++  +++DK+  G++   EL + L +LGEKL + E+ E+ K       
Sbjct: 68  NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124

Query: 504 DDGMIPYAAFLKKVMA 551
            DGM+ Y  F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 57.2 bits (132), Expect = 3e-09
 Identities = 37/146 (25%), Positives = 71/146 (48%)
 Frame = +3

Query: 114 SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 293
           SK   +    AF +YD +  G I   ++G +LR+L  N T A +                
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63

Query: 294 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 473
             F+     + K ++  + E++++  +++DK+ +G +  A+    +  LGEKL D+EV  
Sbjct: 64  MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118

Query: 474 VTKDCMDPEDDDGMIPYAAFLKKVMA 551
           + ++  DP  + G   Y  F++++MA
Sbjct: 119 MVQEA-DP-TNSGSFDYYDFVQRIMA 142


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 31.9 bits (69), Expect = 0.12
 Identities = 33/161 (20%), Positives = 63/161 (39%), Gaps = 1/161 (0%)
 Frame = +3

Query: 69  RPGGNRKTPHKMSDLSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIX 248
           R GG      ++++  + D+  A   F ++D +    ID   L   +RAL  N   + + 
Sbjct: 20  RLGGYAPLRVEITEEQRQDINEA---FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVL 76

Query: 249 XXXXXXXXXXXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTH 425
                             F+ + ++   ++D    E+     +L+D +E G +    L  
Sbjct: 77  KILRDFDKTGKGYLQMEDFVRVMTEKIVERDP--LEEIKRAFELFDDDETGKISLRNLRR 134

Query: 426 TLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVM 548
               L E +DD E+  + ++     D DG I    F+  +M
Sbjct: 135 VAKELNENIDDQELEAMIEEF--DLDQDGEINEQEFIAIMM 173


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.9 bits (59), Expect = 1.9
 Identities = 13/31 (41%), Positives = 15/31 (48%)
 Frame = -3

Query: 704  APPEELSPPRALPAPVPQSRASVF*GPSHRT 612
            APP    PP A P P+P S A     P  R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 27.1 bits (57), Expect = 3.3
 Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
 Frame = +3

Query: 102 MSDLSKNDVER-ASFAFSIYDFEGKGKID 185
           +++L   DV R  SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633


>SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 178

 Score = 26.2 bits (55), Expect = 5.8
 Identities = 23/120 (19%), Positives = 49/120 (40%), Gaps = 1/120 (0%)
 Frame = +1

Query: 4   ELEPSRRKSYGAPLLV*TSASEDREGTEKHHTK*ATSARTTLKGRLSPSQSTTLKAKAR- 180
           +L+  R + +GA        +       ++HT   T A+   KG   P++    KAK   
Sbjct: 15  KLDQHRSRCHGAYFTCIDCNTTFERTDYRNHTSCMTEAQRYQKGLYRPTKKELKKAKMNG 74

Query: 181 SMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHSKSSFPSTAKQRKTKTRERMKIS 360
           +  ++  +S     Q     P+++S+ +  + +    SK    S+  ++     +  +IS
Sbjct: 75  NAVNSKELSPNTDNQNTPAGPTKHSLDENEKDKENKKSKKETVSSPAEQLLALTQNQEIS 134


>SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 182

 Score = 26.2 bits (55), Expect = 5.8
 Identities = 13/33 (39%), Positives = 14/33 (42%)
 Frame = -2

Query: 828 SQKVESCTKQLILCCSYKKGVCPYXRCYTGCRR 730
           S   ESC K+   CC  KK  C       GC R
Sbjct: 74  SDDEESCEKKKPKCCEKKKPKCCESEQNNGCGR 106


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 7.6
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = +3

Query: 438 LGEKLDDSEVAEVTKDCMDPED 503
           L EK+ D +   +  DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,068,623
Number of Sequences: 5004
Number of extensions: 55942
Number of successful extensions: 174
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)