Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P29_F_F22
(858 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 449 e-128
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 434 e-124
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 385 e-109
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 352 3e-99
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 2e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.6
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.6
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.6
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.8
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 449 bits (1106), Expect = e-128
Identities = 210/217 (96%), Positives = 212/217 (97%)
Frame = +1
Query: 145 IYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYV 324
IYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYV
Sbjct: 28 IYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYV 87
Query: 325 TIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQ 504
TIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQ
Sbjct: 88 TIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQ 147
Query: 505 LIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTK 684
LIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+K
Sbjct: 148 LIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSK 207
Query: 685 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDK 795
MPWFKGW VERKEGK +GKCLIEALDAILPP RPTDK
Sbjct: 208 MPWFKGWTVERKEGKVEGKCLIEALDAILPPTRPTDK 244
Score = 58.4 bits (135), Expect = 8e-11
Identities = 26/27 (96%), Positives = 26/27 (96%)
Frame = +3
Query: 63 MGKEKTHINIVVIGHVDSGKSTTTGHL 143
MGKEK HINIVVIGHVDSGKSTTTGHL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHL 27
Score = 37.5 bits (83), Expect = 2e-04
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = +3
Query: 804 RLPLQDVYXIGGIGTVP 854
RLPLQDVY IGGIGTVP
Sbjct: 247 RLPLQDVYKIGGIGTVP 263
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 434 bits (1069), Expect = e-124
Identities = 202/217 (93%), Positives = 209/217 (96%)
Frame = +1
Query: 145 IYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYV 324
IYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFET+KYYV
Sbjct: 28 IYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYV 87
Query: 325 TIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQ 504
TIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNGQTREHALLAFTLGVKQ
Sbjct: 88 TIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQ 147
Query: 505 LIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTK 684
LIVGVNKMD T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWHGDNMLEPS K
Sbjct: 148 LIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPK 207
Query: 685 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDK 795
PW+KGW+VERK+G ADGK LIEALDAILPP+RPTDK
Sbjct: 208 TPWYKGWKVERKDGNADGKTLIEALDAILPPSRPTDK 244
Score = 58.4 bits (135), Expect = 8e-11
Identities = 26/27 (96%), Positives = 26/27 (96%)
Frame = +3
Query: 63 MGKEKTHINIVVIGHVDSGKSTTTGHL 143
MGKEK HINIVVIGHVDSGKSTTTGHL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHL 27
Score = 37.5 bits (83), Expect = 2e-04
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = +3
Query: 804 RLPLQDVYXIGGIGTVP 854
RLPLQDVY IGGIGTVP
Sbjct: 247 RLPLQDVYKIGGIGTVP 263
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 385 bits (947), Expect = e-109
Identities = 180/187 (96%), Positives = 182/187 (97%)
Frame = +1
Query: 235 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 414
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 415 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 594
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 595 IKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILP 774
IKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCLIEALDAILP
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILP 180
Query: 775 PARPTDK 795
P RPTDK
Sbjct: 181 PTRPTDK 187
Score = 37.5 bits (83), Expect = 2e-04
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = +3
Query: 804 RLPLQDVYXIGGIGTVP 854
RLPLQDVY IGGIGTVP
Sbjct: 190 RLPLQDVYKIGGIGTVP 206
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 352 bits (865), Expect = 3e-99
Identities = 164/171 (95%), Positives = 166/171 (97%)
Frame = +1
Query: 283 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 462
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 463 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 642
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 643 SGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDK 795
SGWHGDNMLE S+KMPWFKGW VERKEGK +GKCLIEALDAILPP RPTDK
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTRPTDK 171
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.5 bits (83), Expect = 2e-04
Identities = 36/117 (30%), Positives = 52/117 (44%)
Frame = +1
Query: 322 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 501
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 502 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 672
+IV +NK+D + ++E K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.68
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +3
Query: 75 KTHINIVVIGHVDSGKST 128
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.6
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +3
Query: 84 INIVVIGHVDSGKST 128
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.6
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 76 FSLPIFG*SRITNCV*Y 26
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.6
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 307 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 408
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.8
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 307 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 408
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 255,357
Number of Sequences: 438
Number of extensions: 5539
Number of successful extensions: 24
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 27673956
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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