Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P29_F_E20
(835 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharo... 279 3e-76
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 275 4e-75
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 31 0.27
SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 31 0.27
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 29 1.1
SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 27 2.5
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 27 2.5
SPAC3C7.03c |rhp55||RecA family ATPase Rhp55|Schizosaccharomyces... 27 3.3
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 27 4.3
SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual 27 4.3
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 26 5.7
SPAC869.08 |pcm2||protein-L-isoaspartate O-methyltransferase |Sc... 26 7.6
SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pomb... 26 7.6
>SPAC24C9.12c |||glycine hydroxymethyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 279 bits (684), Expect = 3e-76
Identities = 128/214 (59%), Positives = 161/214 (75%)
Frame = +1
Query: 109 LWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGG 288
L E DP + +I+ E DRQR+ + +IASENFTS V+ L S + NKYSEG P RYYGG
Sbjct: 12 LKEQDPTVAEIMRHEADRQRSSVVLIASENFTSRAVMDALGSVMSNKYSEGYPGARYYGG 71
Query: 289 NEYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPHGRIMGLDLPD 468
N++ID+IE L Q R+L A+ L +WGVNVQ SGSPAN VY I+ PHGR+MGLDLP
Sbjct: 72 NKFIDQIETLCQERALAAFNLDPAKWGVNVQCLSGSPANMQVYQAIMPPHGRLMGLDLPS 131
Query: 469 GGHLTHGFFTATKKISATSIFFESMPYKVDPKSGLIDYDKLAETAKLFKPRLIIAGMSCY 648
GGHL+HG+ T TKKISA S +FESMPY+VDP +GLIDYD L A+LF+P++++AG S Y
Sbjct: 132 GGHLSHGYQTDTKKISAVSTYFESMPYRVDPNTGLIDYDMLEHDAQLFRPKILVAGTSAY 191
Query: 649 SRCLDYKRFREIADANGAYLMADMAHVSGLVAAG 750
R +DY R R+IAD+ AYL+ DMAH+SGLV+AG
Sbjct: 192 CRLIDYARMRQIADSVNAYLVVDMAHISGLVSAG 225
Score = 42.3 bits (95), Expect = 8e-05
Identities = 16/20 (80%), Positives = 19/20 (95%)
Frame = +3
Query: 759 APFEYCDIVTTTTHKTLRGP 818
+PFEY D+VTTTTHK+LRGP
Sbjct: 229 SPFEYADVVTTTTHKSLRGP 248
>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 472
Score = 275 bits (675), Expect = 4e-75
Identities = 124/220 (56%), Positives = 161/220 (73%)
Frame = +1
Query: 91 KLLNSNLWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPN 270
KLL + L E DP ++ I+ EK RQ+ + +IASENFTS V+ L S + NKYSEG P
Sbjct: 12 KLLKAPLAECDPTVYKILESEKSRQKESIALIASENFTSRAVMDALGSIMQNKYSEGYPG 71
Query: 271 QRYYGGNEYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPHGRIM 450
RYYGGNE+ID+ E L Q R+LEA+ L E+WGVNVQP+SGSPAN Y +++PH R+M
Sbjct: 72 ARYYGGNEFIDQAERLCQTRALEAFHLDGEKWGVNVQPHSGSPANLQAYQAVMKPHDRLM 131
Query: 451 GLDLPDGGHLTHGFFTATKKISATSIFFESMPYKVDPKSGLIDYDKLAETAKLFKPRLII 630
GLDLP GGHL+HGF T K ISA S +F +MPY V+ ++G+IDYD L + A F+P++I+
Sbjct: 132 GLDLPHGGHLSHGFSTPQKAISAVSTYFSTMPYNVNKETGIIDYDSLEKAAIQFRPKVIV 191
Query: 631 AGMSCYSRCLDYKRFREIADANGAYLMADMAHVSGLVAAG 750
AG S Y+R +DYKR R+I + AYL+ DMAH+SGLVAAG
Sbjct: 192 AGASAYARLVDYKRMRKITEMCNAYLLCDMAHISGLVAAG 231
Score = 42.7 bits (96), Expect = 6e-05
Identities = 17/20 (85%), Positives = 19/20 (95%)
Frame = +3
Query: 759 APFEYCDIVTTTTHKTLRGP 818
+PFEY DIVTTTTHK+LRGP
Sbjct: 235 SPFEYADIVTTTTHKSLRGP 254
>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 30.7 bits (66), Expect = 0.27
Identities = 16/62 (25%), Positives = 33/62 (53%)
Frame = +1
Query: 145 VKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGGNEYIDEIEILAQ 324
V+E+D L+M+ EN + + + ++CLH+ + + R Y G++ +D + +L
Sbjct: 957 VEERDPPSPLLQML--ENNSKSVIGENWTTCLHSSLVDNLGKYRKYDGSKILDILRVLRN 1014
Query: 325 NR 330
R
Sbjct: 1015 KR 1016
>SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit
Rev3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1480
Score = 30.7 bits (66), Expect = 0.27
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +1
Query: 535 ESMPYKVDPKSGLIDYDKLAETAKLFKPRLIIAGMSCYSRCL 660
E++P ++PKS L + + + P +IIA CYS CL
Sbjct: 934 EALPLVMEPKSDLYNNPVVVLDFQSLYPSIIIAYNLCYSTCL 975
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 28.7 bits (61), Expect = 1.1
Identities = 20/74 (27%), Positives = 37/74 (50%), Gaps = 3/74 (4%)
Frame = +1
Query: 220 QCLSSCLH-NKYSEGMPNQRYYG-GNEY-IDEIEILAQNRSLEAYRLKSEEWGVNVQPYS 390
+C+S + + ++ + +Q Y G N++ I E+ + N+S +A + +G PYS
Sbjct: 1762 ECVSKDIEVDNFALNLQSQSYEGDSNDFGIREVPLSDANQSSQADSTSIDRYG----PYS 1817
Query: 391 GSPANFAVYTGIVE 432
NF+ Y VE
Sbjct: 1818 SQKVNFSKYKDFVE 1831
>SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 27.5 bits (58), Expect = 2.5
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
Frame = +1
Query: 163 QRAGLEMIASENFTSVPVLQCLSSCLHNKY--SEGMPNQRYYGGNEYIDEIEIL 318
Q+ L I S T L CLS CL Y G+ + + G E ++ +EIL
Sbjct: 192 QKLSLLSIQSNRITQFENLACLSHCLRELYVSHNGLTS---FSGIEVLENLEIL 242
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 27.5 bits (58), Expect = 2.5
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 1/55 (1%)
Frame = +1
Query: 394 SPANFAVYTGIVEPHGRIMG-LDLPDGGHLTHGFFTATKKISATSIFFESMPYKV 555
S A ++V T +++ R G + + D GH+ H F I+ I FES P+K+
Sbjct: 1716 SMAAYSVITYLLQFKDRHNGNIMIDDQGHILHIDFGFIFDIAPGGITFESAPFKL 1770
>SPAC3C7.03c |rhp55||RecA family ATPase Rhp55|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 350
Score = 27.1 bits (57), Expect = 3.3
Identities = 16/37 (43%), Positives = 21/37 (56%)
Frame = -1
Query: 748 LQQPVQIREPYQPSDKLHLRLRSHGTSYNRDILSNSS 638
L P+Q+ P P D +LRLR TS + LS+SS
Sbjct: 151 LSMPIQLAYPTSPEDYAYLRLR-RNTS-KKSSLSDSS 185
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 26.6 bits (56), Expect = 4.3
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = -3
Query: 605 FAVSASLS*SINPLLGSTLYGMLSKNIDVAD--IFLVAVKKPW 483
+AV SL S +GS LYG + K+ AD + ++AV W
Sbjct: 624 YAVIFSLFLSFGITIGSALYGWMDKDATDADTCMSIIAVSPYW 666
>SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual
Length = 510
Score = 26.6 bits (56), Expect = 4.3
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = -3
Query: 188 AIISSPAR*RSFSLTIISKSSGSASQRLLFSNLAL 84
+I+ S + S LT + KSSG AS + FSN +L
Sbjct: 397 SILQSDSLMISTQLTSVQKSSGFASYSVQFSNCSL 431
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 26.2 bits (55), Expect = 5.7
Identities = 14/55 (25%), Positives = 32/55 (58%)
Frame = -1
Query: 358 PILICMLPETCSVPVSLFHQYIHSPHSTVDWACLQNICYEDMNSGTEVQELM*NF 194
P++ +L ++P+ LF + IH T+ ++C I E+++ +++EL+ N+
Sbjct: 1758 PVIATILDSILNLPLELFSENIH----TLYFSCCSMIAKENLDD--QLRELLKNY 1806
>SPAC869.08 |pcm2||protein-L-isoaspartate O-methyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 230
Score = 25.8 bits (54), Expect = 7.6
Identities = 12/49 (24%), Positives = 22/49 (44%)
Frame = +1
Query: 292 EYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPH 438
+++ E + L R+++A S + + PY SP + I PH
Sbjct: 15 QHLVESKFLTNQRAIKAMNATSRSFYCPLSPYMDSPQSIGYGVTISAPH 63
>SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 955
Score = 25.8 bits (54), Expect = 7.6
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -3
Query: 539 LSKNIDVADIFLVAVKKPWVRCPPSGKSNPIILPWGSTMPV 417
L+ NID+ IFL V P P+G+ +PI+ + P+
Sbjct: 620 LADNIDIVTIFLSLVHPP----SPAGELHPIVRLFQDIWPI 656
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,626,612
Number of Sequences: 5004
Number of extensions: 79349
Number of successful extensions: 235
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 235
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 410448950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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