BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P29_F_D16
(861 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|... 61 2e-10
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 27 3.4
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 27 3.4
SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ... 27 4.5
SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 6.0
SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|c... 26 7.9
>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 532
Score = 61.3 bits (142), Expect = 2e-10
Identities = 47/133 (35%), Positives = 67/133 (50%), Gaps = 4/133 (3%)
Frame = +1
Query: 157 KNVILFVGDGMGPNTVTATRIY----KGGESHRLVYEKFPHVGLLKTYSANKMVPDSSCS 324
K VI+ V DGMGP +++ TR + E +RL ++ +G +T S++ ++ DS+
Sbjct: 60 KFVIMMVSDGMGPGSLSMTRSFVETLNDKEGYRLPLDEHL-IGSSRTRSSSSLITDSAAG 118
Query: 325 ATALFCGVKANQETIGVDASVKHKDCSASLRPEARLKSLASIALKAGKSAGFVTTMRVTH 504
ATA C K +GV + K C L EA A +AG G V T RVT
Sbjct: 119 ATAFSCANKTYNGAVGVLDN--EKPCGTIL--EA--------AKEAGYLTGIVVTSRVTD 166
Query: 505 ATPGPIYAHSADR 543
ATP AH+A+R
Sbjct: 167 ATPASFSAHAANR 179
>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 27.1 bits (57), Expect = 3.4
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = -3
Query: 673 IQEPVCLHL--GLPASLFRASLPQVASRYPCKQQR*QAFCLRIPIYDQHCGRK 521
IQ+ LHL L + SL +AS+Y C++Q + R+P +C +K
Sbjct: 61 IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKK 113
>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 27.1 bits (57), Expect = 3.4
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = -3
Query: 673 IQEPVCLHL--GLPASLFRASLPQVASRYPCKQQR*QAFCLRIPIYDQHCGRK 521
IQ+ LHL L + SL +AS+Y C++Q + R+P +C +K
Sbjct: 61 IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKK 113
>SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 582
Score = 26.6 bits (56), Expect = 4.5
Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = -2
Query: 752 SFMRSVPSFXMQDHVSNGSSLVPVLLDTRTCLPP-PRITCKSFPGQSSTS 606
+F +VP++ Q N ++LVP + LPP P +T S P +++S
Sbjct: 263 NFPTTVPNYPFQQPSYNPNALVPSYTTLVSQLPPSPCLTVSSGPLSTASS 312
>SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 279
Score = 26.2 bits (55), Expect = 6.0
Identities = 10/21 (47%), Positives = 16/21 (76%)
Frame = +2
Query: 2 LVVAMFPRILPAVLCYLRGKM 64
LVV + ++LPAVLCY+ ++
Sbjct: 198 LVVKLGLKVLPAVLCYVNSQL 218
>SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 625
Score = 25.8 bits (54), Expect = 7.9
Identities = 13/27 (48%), Positives = 14/27 (51%)
Frame = +3
Query: 477 FCDNYASHPCDPGSHLRPQC*S*MGMR 557
FC Y S PC PG +PQ S M R
Sbjct: 500 FCQFYVSEPCKPGG--KPQLSSMMYQR 524
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,680,884
Number of Sequences: 5004
Number of extensions: 75717
Number of successful extensions: 162
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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