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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P29_F_D16
         (861 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|...    61   2e-10
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|...    27   3.4  
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz...    27   3.4  
SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ...    27   4.5  
SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces ...    26   6.0  
SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|c...    26   7.9  

>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 532

 Score = 61.3 bits (142), Expect = 2e-10
 Identities = 47/133 (35%), Positives = 67/133 (50%), Gaps = 4/133 (3%)
 Frame = +1

Query: 157 KNVILFVGDGMGPNTVTATRIY----KGGESHRLVYEKFPHVGLLKTYSANKMVPDSSCS 324
           K VI+ V DGMGP +++ TR +       E +RL  ++   +G  +T S++ ++ DS+  
Sbjct: 60  KFVIMMVSDGMGPGSLSMTRSFVETLNDKEGYRLPLDEHL-IGSSRTRSSSSLITDSAAG 118

Query: 325 ATALFCGVKANQETIGVDASVKHKDCSASLRPEARLKSLASIALKAGKSAGFVTTMRVTH 504
           ATA  C  K     +GV  +   K C   L  EA        A +AG   G V T RVT 
Sbjct: 119 ATAFSCANKTYNGAVGVLDN--EKPCGTIL--EA--------AKEAGYLTGIVVTSRVTD 166

Query: 505 ATPGPIYAHSADR 543
           ATP    AH+A+R
Sbjct: 167 ATPASFSAHAANR 179


>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
           Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
 Frame = -3

Query: 673 IQEPVCLHL--GLPASLFRASLPQVASRYPCKQQR*QAFCLRIPIYDQHCGRK 521
           IQ+   LHL   L   +   SL  +AS+Y C++Q  +    R+P    +C +K
Sbjct: 61  IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKK 113


>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
           Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
 Frame = -3

Query: 673 IQEPVCLHL--GLPASLFRASLPQVASRYPCKQQR*QAFCLRIPIYDQHCGRK 521
           IQ+   LHL   L   +   SL  +AS+Y C++Q  +    R+P    +C +K
Sbjct: 61  IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKK 113


>SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 582

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
 Frame = -2

Query: 752 SFMRSVPSFXMQDHVSNGSSLVPVLLDTRTCLPP-PRITCKSFPGQSSTS 606
           +F  +VP++  Q    N ++LVP      + LPP P +T  S P  +++S
Sbjct: 263 NFPTTVPNYPFQQPSYNPNALVPSYTTLVSQLPPSPCLTVSSGPLSTASS 312


>SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 279

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 10/21 (47%), Positives = 16/21 (76%)
 Frame = +2

Query: 2   LVVAMFPRILPAVLCYLRGKM 64
           LVV +  ++LPAVLCY+  ++
Sbjct: 198 LVVKLGLKVLPAVLCYVNSQL 218


>SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 625

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 13/27 (48%), Positives = 14/27 (51%)
 Frame = +3

Query: 477 FCDNYASHPCDPGSHLRPQC*S*MGMR 557
           FC  Y S PC PG   +PQ  S M  R
Sbjct: 500 FCQFYVSEPCKPGG--KPQLSSMMYQR 524


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,680,884
Number of Sequences: 5004
Number of extensions: 75717
Number of successful extensions: 162
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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