Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P29_F_B22
(779 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 218 8e-58
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 55 1e-08
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 35 0.015
SPBC530.09c |||cation dependent mannose-6-phosphate cargo recept... 30 0.43
SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo... 27 3.0
SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pomb... 27 4.0
SPCC1682.13 |||SWIRM domain protein|Schizosaccharomyces pombe|ch... 26 5.3
SPAC1093.04c |||tRNA nucleotidyltransferase |Schizosaccharomyces... 26 5.3
SPAC4G9.19 |||DNAJ domain protein DNAJB family|Schizosaccharomyc... 26 5.3
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 218 bits (532), Expect = 8e-58
Identities = 104/202 (51%), Positives = 144/202 (71%), Gaps = 3/202 (1%)
Frame = +2
Query: 119 WEKQQKKTFTAWCNSHLRKAGT-GIDNIEDDFRNGLKLMLLLEVISGETLPKPDRG-KMR 292
W+ Q +TFT W N+ L + ++ D +G+ L+ LLE+I E L + +R +MR
Sbjct: 6 WQSVQNRTFTKWFNTKLSSRDLPSVFDLRKDLSDGILLIQLLEIIGDENLGRYNRNPRMR 65
Query: 293 FHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEEMTA 472
H++ NVNKAL++I SKG+ L +IG +IVDGNLK+ LG+IWT+ILRF I DI+ E +TA
Sbjct: 66 VHRLENVNKALEYIKSKGMPLTNIGPADIVDGNLKLILGLIWTLILRFTIADINEEGLTA 125
Query: 473 KEGLLLWCQRKTAPY-KNVNVQNFHLSFKDGLAFCALIHRHRPDLIDYSKLSKDNPLENL 649
KEGLLLWCQRKTA Y V+VQ+F S+ +GLAFCALIH+HRPDL+DY+KL K N N+
Sbjct: 126 KEGLLLWCQRKTANYHPEVDVQDFTRSWTNGLAFCALIHQHRPDLLDYNKLDKKNHRANM 185
Query: 650 NTAFDVXEKYLDIPRMLXPDDL 715
AFD+ +K + IPR++ +D+
Sbjct: 186 QLAFDIAQKSIGIPRLIEVEDV 207
Score = 30.7 bits (66), Expect = 0.24
Identities = 11/16 (68%), Positives = 15/16 (93%)
Frame = +3
Query: 729 KPDERAIMTYVSCYYH 776
+PDER+IMTYV+ Y+H
Sbjct: 212 RPDERSIMTYVAEYFH 227
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 614
Score = 54.8 bits (126), Expect = 1e-08
Identities = 55/247 (22%), Positives = 110/247 (44%), Gaps = 18/247 (7%)
Frame = +2
Query: 32 VINNYDGLYPDGYMEQEEEWXREGLLHPAWEKQQKKTFTAWCNSHLRKAGTGIDNIEDDF 211
+ N + GL P + +EE+ E A +++ + FT W NS I + ++
Sbjct: 359 LFNTHPGLEP---LNEEEKPEIEPF--DAEGEREARVFTLWLNS--LDVTPSIHDFFNNL 411
Query: 212 RNGLKLMLLLEVISGETL--------PKPDRGKMRFHKIANVNKALDFIASKGVKLVSIG 367
R+GL L+ + I+ T+ P MRF + N N A+D ++G LV I
Sbjct: 412 RDGLILLQAYDKITPNTVNWKKVNKAPASGDEMMRFKAVENCNYAVDLGKNQGFSLVGIQ 471
Query: 368 AEEIVDGNLKMTLGMIWTII---LRFAIQDISVEEMTAKEG-LLLWCQRKTAP-YKNVNV 532
+I DG+ +TL ++W ++ + + +S T + ++ W A K +
Sbjct: 472 GADITDGSRTLTLALVWQMMRMNITKTLHSLSRGGKTLSDSDMVAWANSMAAKGGKGSQI 531
Query: 533 QNFH-LSFKDGLAFCALIHRHRPDLIDYSKL----SKDNPLENLNTAFDVXEKYLDIPRM 697
++F S G+ ++H + + +DY+ + +++ ++N A + K L
Sbjct: 532 RSFRDPSISTGVFVLDVLHGIKSEYVDYNLVTDGSTEELAIQNARLAISIARK-LGAVIF 590
Query: 698 LXPDDLI 718
+ P+D++
Sbjct: 591 ILPEDIV 597
Score = 37.5 bits (83), Expect = 0.002
Identities = 47/200 (23%), Positives = 84/200 (42%), Gaps = 28/200 (14%)
Frame = +2
Query: 203 DDFRNGLKLMLLLEVISGETLPKPDRGKMRFHK-------IANVNKALDFI-ASKGVKLV 358
D ++GL L L+ +T+ + K R +K I N N ++ A G+ +
Sbjct: 147 DQCKDGLILSKLINDSVPDTIDERVLNKQRNNKPLDNFKCIENNNVVINSAKAMGGISIT 206
Query: 359 SIGAEEIVDGNLKMTLGMIWTIILRFAIQDISV-----------EEMTAKEGLLLWCQRK 505
+IGA +I++G + LG++W II R + I + E+ T + L L ++
Sbjct: 207 NIGAGDILEGREHLILGLVWQIIRRGLLGKIDITLHPELYRLLEEDETLDQFLRLPPEKI 266
Query: 506 TAPYKNVN---------VQNFHLSFKDGLAFCALIHRHRPDLIDYSKLSKDNPLENLNTA 658
+ N + V NF DG + L+++ P+L + L + L+
Sbjct: 267 LLRWFNYHLKAANWPRTVSNFSKDVSDGENYTVLLNQLAPELCSRAPLQTTDVLQRAEQV 326
Query: 659 FDVXEKYLDIPRMLXPDDLI 718
EK LD + L P ++
Sbjct: 327 LQNAEK-LDCRKYLTPTAMV 345
Score = 26.2 bits (55), Expect = 5.3
Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 5/46 (10%)
Frame = +2
Query: 524 VNVQNFHL--SFKDGLAFCALIHRHRPDLIDYSKLSK---DNPLEN 646
+N + F KDGL LI+ PD ID L+K + PL+N
Sbjct: 138 INTETFEFFDQCKDGLILSKLINDSVPDTIDERVLNKQRNNKPLDN 183
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 34.7 bits (76), Expect = 0.015
Identities = 27/98 (27%), Positives = 48/98 (48%), Gaps = 3/98 (3%)
Frame = +2
Query: 200 EDDFRNGLKLMLLLEVISGETLPKPD-RGKMRFHKIANVNKALDFIASKGV-KLVSIGAE 373
E RNG+ L LL++ + L K +++F N+NK LDFI G+ ++
Sbjct: 66 EQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNINKFLDFIHGIGLPEIFHFELT 125
Query: 374 EIVDG-NLKMTLGMIWTIILRFAIQDISVEEMTAKEGL 484
+I +G NL + I + ++QD++ + + E L
Sbjct: 126 DIYEGKNLPKVIYCIHALSYFLSMQDLAPPLIKSDENL 163
>SPBC530.09c |||cation dependent mannose-6-phosphate cargo receptor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 29.9 bits (64), Expect = 0.43
Identities = 18/55 (32%), Positives = 26/55 (47%)
Frame = +2
Query: 479 GLLLWCQRKTAPYKNVNVQNFHLSFKDGLAFCALIHRHRPDLIDYSKLSKDNPLE 643
GL L+ Q + A + + F + D FCAL H + + D S L +DN E
Sbjct: 13 GLTLFSQFQRAFGLTITRRGFKVQESDEEPFCALHHPNTGEYFDLSGLIRDNTSE 67
>SPAC3G6.01 |hrp3||ATP-dependent DNA helicase
Hrp3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1388
Score = 27.1 bits (57), Expect = 3.0
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +2
Query: 5 AVTMMMDNGVINNYDGLYPDGYMEQEEEWXREGLLHPAWE 124
A+ +++D+ +I +DG P E +W LH WE
Sbjct: 192 AIDVVLDHRLIEGHDGSTPSEDYEFLIKWVNFSHLHCTWE 231
>SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 26.6 bits (56), Expect = 4.0
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +1
Query: 223 EAHVAAGGHLRRDSAQARPW 282
EAHVAA +LR+ RPW
Sbjct: 250 EAHVAALDYLRQHFVSCRPW 269
>SPCC1682.13 |||SWIRM domain protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 272
Score = 26.2 bits (55), Expect = 5.3
Identities = 10/36 (27%), Positives = 17/36 (47%)
Frame = +2
Query: 59 PDGYMEQEEEWXREGLLHPAWEKQQKKTFTAWCNSH 166
P +++QE W R +HP + ++ W N H
Sbjct: 59 PKLWLQQELIWLRRKRIHPIPKARRNNHVGRWANRH 94
>SPAC1093.04c |||tRNA nucleotidyltransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 500
Score = 26.2 bits (55), Expect = 5.3
Identities = 22/85 (25%), Positives = 38/85 (44%), Gaps = 7/85 (8%)
Frame = +2
Query: 299 KIANVNKALDFIASKGVKLVSIGAEEIVDGNLKM----TLGMIWTIILRFAI---QDISV 457
K N N+AL I S G+ G EI L+ +L +I I F +D+S+
Sbjct: 242 KHCNTNRALKIIHSLGMFACIFGPLEIHTKKLQSKNIESLSLIPYAIDLFGYLQKKDVSI 301
Query: 458 EEMTAKEGLLLWCQRKTAPYKNVNV 532
+ +++ + W T P+ N ++
Sbjct: 302 KNLSSSSKYIFWLAIATLPWYNWSI 326
>SPAC4G9.19 |||DNAJ domain protein DNAJB family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 26.2 bits (55), Expect = 5.3
Identities = 16/53 (30%), Positives = 24/53 (45%), Gaps = 5/53 (9%)
Frame = +2
Query: 587 RHRPDLIDYSKLSKDNPLENLN-----TAFDVXEKYLDIPRMLXPDDLINHAQ 730
R YS+L P E L TA D+ KY+++ + PD + N +Q
Sbjct: 17 RRAASFTSYSELKSLTPYEILELPRTCTANDIKRKYIELVKKHHPDKMKNASQ 69
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,843,717
Number of Sequences: 5004
Number of extensions: 56994
Number of successful extensions: 222
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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