Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P28_pT_M11
(770 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Sch... 218 8e-58
SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein L5|Schizo... 216 3e-57
SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pomb... 29 0.56
SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces p... 29 0.97
SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces pomb... 29 0.97
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 28 1.3
SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual 27 3.0
SPAC140.04 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.0
SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces po... 27 3.9
SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 27 3.9
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 26 5.2
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 26 5.2
SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po... 26 5.2
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 26 6.9
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 9.1
SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 25 9.1
SPBC4F6.16c |ero11||ER oxidoreductin Ero1a|Schizosaccharomyces p... 25 9.1
>SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein
L5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 294
Score = 218 bits (532), Expect = 8e-58
Identities = 108/241 (44%), Positives = 150/241 (62%), Gaps = 2/241 (0%)
Frame = -3
Query: 765 PXYRLIVRLSNKDVTCQVAYSRIEGDHIVCAAYSHELPRYGVKVGLTNYAAAYSTGXXXX 586
P YRL+VR SN+ VTCQ+ SR+ GD+++ A+S ELPRYG+K GL N+ AAY+TG
Sbjct: 47 PKYRLVVRFSNRFVTCQIVSSRVNGDYVLAHAHSSELPRYGIKWGLANWTAAYATGLLVA 106
Query: 585 XXXXXXXXXXXXXXXXXXXXXDEYNVEPVDNGPGAFRCYLDVGLARTTTGARVFGAMKGA 406
+ E +++GP F+ +LDVGL RT+TG+RVFGAMKGA
Sbjct: 107 RRALAKVGLADKYEGVTEPEGEFELTEAIEDGPRPFKVFLDVGLKRTSTGSRVFGAMKGA 166
Query: 405 VDGGLNVPHSIKRFPGYDAESKKFNAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKY 226
DGGL +PHS RFPG+D E+++ + E R +I+G HVAEYM L DDE+ +++QFS
Sbjct: 167 SDGGLFIPHSPNRFPGFDIETEELDDETLRKYIYGGHVAEYMEMLIDDDEERYQKQFSGL 226
Query: 225 IKLGVTADAIEAIYKKAHEAIRADPSHKK--KELKKDSVKQKRWNKRKLTLAERKNRIKQ 52
I G+ +D +E IY +A+ IR DPS +K K+ + ++ +RKLT ERK R
Sbjct: 227 IADGIESDQLEDIYAEAYAKIREDPSFQKSGKDAAAFKAESLKYTQRKLTAEERKERFNA 286
Query: 51 K 49
K
Sbjct: 287 K 287
>SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein
L5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 294
Score = 216 bits (528), Expect = 3e-57
Identities = 108/241 (44%), Positives = 149/241 (61%), Gaps = 2/241 (0%)
Frame = -3
Query: 765 PXYRLIVRLSNKDVTCQVAYSRIEGDHIVCAAYSHELPRYGVKVGLTNYAAAYSTGXXXX 586
P YRL+VR SN+ VTCQ+ SR+ GD+++ A+S ELPRYG+K GL N+ AAY+TG
Sbjct: 47 PKYRLVVRFSNRFVTCQIVSSRVNGDYVLAHAHSSELPRYGIKWGLANWTAAYATGLLVA 106
Query: 585 XXXXXXXXXXXXXXXXXXXXXDEYNVEPVDNGPGAFRCYLDVGLARTTTGARVFGAMKGA 406
+ E +++GP F+ +LDVGL RT+TG+RVFGAMKGA
Sbjct: 107 RRALAKVGLADKYEGVTEPEGEFELTEAIEDGPRPFKVFLDVGLKRTSTGSRVFGAMKGA 166
Query: 405 VDGGLNVPHSIKRFPGYDAESKKFNAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKY 226
DGGL +PHS RFPG+D E+++ + E R +I+G HVAEYM L DDE+ +++QFS
Sbjct: 167 SDGGLFIPHSPNRFPGFDIETEELDDETLRKYIYGGHVAEYMEMLIDDDEERYQKQFSGL 226
Query: 225 IKLGVTADAIEAIYKKAHEAIRADPSHKK--KELKKDSVKQKRWNKRKLTLAERKNRIKQ 52
I G+ +D +E IY +A+ IR DPS +K K+ + + +RKLT ERK R
Sbjct: 227 IADGIESDQLEDIYAEAYAKIREDPSFQKSGKDAAAFKAESLKHTQRKLTAEERKERFNA 286
Query: 51 K 49
K
Sbjct: 287 K 287
>SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 508
Score = 29.5 bits (63), Expect = 0.56
Identities = 19/53 (35%), Positives = 27/53 (50%)
Frame = -3
Query: 198 IEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKAS 40
IE KK+ + AD KKE K+ K+ + +KRK +E K+KK S
Sbjct: 451 IEKKKKKSSKLKEADGESSKKEKKEKKDKKHKKSKRKSEESEDGESPKKKKKS 503
>SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 480
Score = 28.7 bits (61), Expect = 0.97
Identities = 23/94 (24%), Positives = 45/94 (47%)
Frame = -3
Query: 288 EYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQ 109
E +EQ +S +R FS+ +L E + KK EA R +++ KK+ +++
Sbjct: 86 EEQNLIEQSITES-QRIFSEKQRLEQERFNRELLEKKRIEAERQRLKDEEERRKKELMEK 144
Query: 108 KRWNKRKLTLAERKNRIKQKKASCIQETAGDRRK 7
++ K ++ L E + + ++ QE +RK
Sbjct: 145 EKKEKERIRLIEEQKHKENEQRRLKQEQIDAKRK 178
>SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 497
Score = 28.7 bits (61), Expect = 0.97
Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = -3
Query: 171 EAIRADPSHKKKELKKDSVKQKRWNKRKL-TLAERKNRIKQKKAS 40
E I PS K+K+ KK+ K+K KR +E + K+KK S
Sbjct: 452 ETISEKPSKKEKKDKKEKKKEKSKKKRSADDASEEVKKSKKKKKS 496
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 28.3 bits (60), Expect = 1.3
Identities = 18/69 (26%), Positives = 38/69 (55%), Gaps = 1/69 (1%)
Frame = -3
Query: 210 TADAIEAIYKK-AHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASCI 34
T D I+ + K+ E ++ + ++ +LKK + + +K K+++ E+K R Q K + +
Sbjct: 1636 THDNIDDMIKQQVEEKLKENSANFDVKLKKVVAETEFRSKAKISVYEKKTRDLQNKITQL 1695
Query: 33 QETAGDRRK 7
+ET + K
Sbjct: 1696 EETIENLNK 1704
Score = 27.5 bits (58), Expect = 2.2
Identities = 13/54 (24%), Positives = 29/54 (53%)
Frame = -3
Query: 267 QDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQK 106
+++++S ++ +K ++ V +KK +E IR+D LK+D K++
Sbjct: 1023 KEEKESSSKELAKQLEDAVREKDSALSFKKDYEKIRSDADRVITSLKEDIEKER 1076
>SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual
Length = 307
Score = 27.1 bits (57), Expect = 3.0
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = +3
Query: 573 EAVFVLIADQLNMLQHNLSDQPSHHNVATHV 665
EAV + + D + L +LSD+ S+ ++A H+
Sbjct: 169 EAVVLRVIDPSSKLAEDLSDEQSYRSLAEHI 199
>SPAC140.04 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 295
Score = 27.1 bits (57), Expect = 3.0
Identities = 15/59 (25%), Positives = 29/59 (49%)
Frame = -3
Query: 183 KKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASCIQETAGDRRK 7
KK +E + SH+K K KRKL + +++ K+ +++ I++ + RK
Sbjct: 27 KKRYEDEKRKISHQKYTDKLLEANDNETTKRKLLVKQKRKSNKEFQSNIIKKRKDEERK 85
>SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 26.6 bits (56), Expect = 3.9
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = -3
Query: 201 AIEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRK 88
++E+ K EA + + +KKE KK+ K+K+ K K
Sbjct: 430 SVESSEKDEDEAAKKEEKRRKKEAKKEK-KEKKEKKEK 466
>SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
Length = 674
Score = 26.6 bits (56), Expect = 3.9
Identities = 18/63 (28%), Positives = 26/63 (41%)
Frame = -3
Query: 210 TADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASCIQ 31
TA IEA+YKKA+ A S K ++ + KR L + NR+ +
Sbjct: 174 TAQLIEAVYKKANIKDAAHDSKNLKSVEGLVIANDADPKRAQMLVHQINRLNSPNILVVN 233
Query: 30 ETA 22
A
Sbjct: 234 HDA 236
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 26.2 bits (55), Expect = 5.2
Identities = 19/58 (32%), Positives = 34/58 (58%), Gaps = 2/58 (3%)
Frame = -3
Query: 180 KAHEAIRADPSHKK-KELKKDSVKQKRWNKRK-LTLAERKNRIKQKKASCIQETAGDR 13
K E I + K+ +ELKK K+K+ +K+K L LA+ + R ++++A + E A +
Sbjct: 571 KLLEEIEEENKRKQERELKKIREKEKKRDKKKQLKLAKEEER-QRREAERLAEQAAQK 627
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 26.2 bits (55), Expect = 5.2
Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 2/63 (3%)
Frame = -3
Query: 201 AIEAIYKKAHEAIRADPSH--KKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASCIQE 28
++EA +K E I + KK LKK ++++ KRK A K+++A+ I
Sbjct: 12 SLEAKNEKPKEVILGEDGKPLSKKALKKLEKEREKEQKRKEREAREAEEKKKREANEIDY 71
Query: 27 TAG 19
+AG
Sbjct: 72 SAG 74
>SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 284
Score = 26.2 bits (55), Expect = 5.2
Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = -3
Query: 270 EQDDEDSFKRQFSKYIKLGVTADA--IEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWN 97
E+ + S K++ K G D +E I KK + HKKK+ + S K+K +
Sbjct: 166 EKRKKHSSKKKSKKKTSTGSALDPKKLEKITKKKKK------EHKKKDKESSSKKRKSGS 219
Query: 96 KRKLTLAERKNRIKQKKAS 40
K ++K ++K K S
Sbjct: 220 SDKEEKKKKKIKLKDKPES 238
>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 207
Score = 25.8 bits (54), Expect = 6.9
Identities = 18/62 (29%), Positives = 33/62 (53%), Gaps = 2/62 (3%)
Frame = -3
Query: 183 KKAHEAIRADPSHKKKE--LKKDSVKQKRWNKRKLTLAERKNRIKQKKASCIQETAGDRR 10
KK E A S K+ E KKD+ + ++W+K T +K + +K+ + ++ A +R
Sbjct: 5 KKRAEKAEAAKSRKQDEEKKKKDAEEDEKWSKGVKT--NKKEQEAEKRKAALERKA-ERE 61
Query: 9 KL 4
+L
Sbjct: 62 RL 63
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 25.4 bits (53), Expect = 9.1
Identities = 18/68 (26%), Positives = 28/68 (41%)
Frame = -3
Query: 336 FNAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRA 157
FNA R+ + A+ D E F + G A+ +IY+ + E IR+
Sbjct: 363 FNASGERSESANVETAQVW----DDREQYFFYEVFPNFNEGSIAEMKSSIYESSQEGIRS 418
Query: 156 DPSHKKKE 133
+ KKE
Sbjct: 419 SSENNKKE 426
>SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 978
Score = 25.4 bits (53), Expect = 9.1
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = -3
Query: 291 AEYMRSLEQDDEDSFKRQFSKYIK 220
+EY+++L D SFK+ F K+I+
Sbjct: 948 SEYLQALSNLDSRSFKQFFQKFIQ 971
>SPBC4F6.16c |ero11||ER oxidoreductin Ero1a|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 467
Score = 25.4 bits (53), Expect = 9.1
Identities = 10/32 (31%), Positives = 20/32 (62%)
Frame = +2
Query: 14 LSPAVSCMQEAFFCLILFFLSANVSLRLFQRF 109
LSP+V+C+++ F I+ A++S + Q +
Sbjct: 206 LSPSVACLEKRMFNRIISGFHASISTHVCQNY 237
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,010,412
Number of Sequences: 5004
Number of extensions: 58854
Number of successful extensions: 274
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 244
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 267
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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