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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P28_pT_I23
         (407 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    30   0.16 
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb...    26   2.6  
SPCC320.08 |||membrane transporter |Schizosaccharomyces pombe|ch...    26   2.6  
SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces p...    25   3.4  
SPAC589.04 |||metaxin 1|Schizosaccharomyces pombe|chr 1|||Manual       25   6.0  
SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase |Schizos...    24   7.9  
SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3...    24   7.9  
SPAC3H1.09c |||vacuolar amino acid transporter |Schizosaccharomy...    24   7.9  
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces...    24   7.9  
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c...    24   7.9  

>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 425

 Score = 29.9 bits (64), Expect = 0.16
 Identities = 12/37 (32%), Positives = 18/37 (48%)
 Frame = -1

Query: 299 NRRCDIQWHFQCHSFNS*RKYS*LERRFWSPANHGDI 189
           N RCD ++HF  H  NS   Y+      + P +H  +
Sbjct: 352 NSRCDTKYHFLGHKCNSCHSYNTCISSIYKPLDHPQV 388


>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1207

 Score = 25.8 bits (54), Expect = 2.6
 Identities = 16/46 (34%), Positives = 21/46 (45%)
 Frame = +1

Query: 1   IKSYKGIRKSFRNKVSVKHLSSSFYSPNVHSGNIKSNLLVTRLSAD 138
           I SY  +R       SVKHL S++  P   S N    + +   SAD
Sbjct: 349 IVSYDLLRAQSTTVASVKHLGSAWLPPRSLSYNPAEKVALLTSSAD 394


>SPCC320.08 |||membrane transporter |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 505

 Score = 25.8 bits (54), Expect = 2.6
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +2

Query: 113 FWLLDYQLTGVILRSAITSWATIS 184
           F +L YQ T ++  S + SWAT++
Sbjct: 224 FVVLAYQYTNMLSASLLDSWATVA 247


>SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 485

 Score = 25.4 bits (53), Expect = 3.4
 Identities = 13/42 (30%), Positives = 24/42 (57%), Gaps = 3/42 (7%)
 Frame = -2

Query: 370 ALCVTVLLLINVSGIKCFN-VTSPSIEGVIFNGT--FNATVL 254
           A+C+  +L++ +  + CF  +  P + G   NGT  F +TV+
Sbjct: 36  AICILGILILAIVKLFCFKAIIFPIVGGSFNNGTNVFQSTVI 77


>SPAC589.04 |||metaxin 1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 271

 Score = 24.6 bits (51), Expect = 6.0
 Identities = 7/15 (46%), Positives = 10/15 (66%)
 Frame = -1

Query: 203 NHGDIHRRWLPNWLW 159
           N  +I ++WLPN  W
Sbjct: 142 NFSEIQKKWLPNMSW 156


>SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 891

 Score = 24.2 bits (50), Expect = 7.9
 Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 2/39 (5%)
 Frame = +3

Query: 294 SIDGEVTLKH--FIPDTLISNSTVTHNAYCRNISVDSIH 404
           +I G + +KH   +  T  SN    +N YCR I   S+H
Sbjct: 211 AISGALLVKHQSLVVYTSDSNFEGHYNHYCRKILWPSLH 249


>SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 449

 Score = 24.2 bits (50), Expect = 7.9
 Identities = 9/23 (39%), Positives = 12/23 (52%)
 Frame = +3

Query: 327 IPDTLISNSTVTHNAYCRNISVD 395
           + DT      +T N+YCR  S D
Sbjct: 424 VKDTSDDKFDITDNSYCREFSAD 446


>SPAC3H1.09c |||vacuolar amino acid transporter |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 656

 Score = 24.2 bits (50), Expect = 7.9
 Identities = 13/40 (32%), Positives = 18/40 (45%)
 Frame = +3

Query: 180 SPMNISVICGTPESPFKSTVFSLRVKTVALKVPLNITPSI 299
           S +   VI   PES F   +  L    + L  PL + P+I
Sbjct: 515 SKVKTVVILNMPESTFTVIIQFLYAIAILLSTPLQLFPAI 554


>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1009

 Score = 24.2 bits (50), Expect = 7.9
 Identities = 12/29 (41%), Positives = 15/29 (51%), Gaps = 1/29 (3%)
 Frame = -1

Query: 245 RKYS*LERRFWSPANHGDIHRRWLPN-WL 162
           R +  LE  F S  N+   HR WL N W+
Sbjct: 554 RVWDILESSFESSKNYKSDHREWLSNPWV 582


>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1111

 Score = 24.2 bits (50), Expect = 7.9
 Identities = 11/39 (28%), Positives = 20/39 (51%)
 Frame = +2

Query: 5   KVIKXSVKASVIKFQLSIYLALSIRQTYILEI*SRIFWL 121
           K  K + K + +K+Q  I  +L + +T I +     +WL
Sbjct: 165 KKSKAAAKTAAVKYQFQIMESLGVPRTEIHKFADAKYWL 203


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,669,258
Number of Sequences: 5004
Number of extensions: 34013
Number of successful extensions: 100
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 140222766
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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