Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P28_pT_E13
(720 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 162 4e-41
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 161 7e-41
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 28 1.2
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch... 28 1.2
SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp... 25 8.2
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 25 8.2
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 162 bits (394), Expect = 4e-41
Identities = 84/165 (50%), Positives = 110/165 (66%), Gaps = 2/165 (1%)
Frame = -3
Query: 715 GKGKMRNRRRIQRKGPLIXFNKDQGXTRAFRNIPGVEXXXXXXXXXXXLAPGGHLGRFVI 536
GKGK+RNRR +QR+GPL+ FN+D G +AFRNIPGVE LAPGGHLGRFVI
Sbjct: 192 GKGKLRNRRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVI 251
Query: 535 WTQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVI 359
WT+SAFG LD +FGS ++ KKN+ LP+ ++N D+TRL+ SDEI+ +++A RV
Sbjct: 252 WTKSAFGLLDSVFGSTTEAAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVK 311
Query: 358 RA-TRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKALAD 227
RA +K NPL N + +LNPYA KA ++L K KA +
Sbjct: 312 RAHVQKKNPLKNKAVLARLNPYAKAY--KANVKLNTGKTPKAAGE 354
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 161 bits (392), Expect = 7e-41
Identities = 83/165 (50%), Positives = 110/165 (66%), Gaps = 2/165 (1%)
Frame = -3
Query: 715 GKGKMRNRRRIQRKGPLIXFNKDQGXTRAFRNIPGVEXXXXXXXXXXXLAPGGHLGRFVI 536
GKGK+RNRR +QR+GPL+ FN+D G +AFRNIPGVE LAPGGHLGRFVI
Sbjct: 192 GKGKLRNRRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVI 251
Query: 535 WTQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVI 359
WT+SAFG LD +FGS ++ KKN+ LP+ ++N D+TRL+ SDEI+ +++A RV
Sbjct: 252 WTKSAFGLLDSVFGSTTEVAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVK 311
Query: 358 RA-TRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKALAD 227
RA +K NPL N + +LNPYA KA +++ K KA +
Sbjct: 312 RAHVQKKNPLKNKAVLSRLNPYAKAY--KANVKINSEKTPKAAGE 354
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 28.3 bits (60), Expect = 1.2
Identities = 27/109 (24%), Positives = 46/109 (42%)
Frame = -3
Query: 475 KQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPY 296
KQK + + Q K LT+ S E + VL +R T+K LTN+ ++K +P
Sbjct: 730 KQKAHLSADQCKQLANVLTQA--SPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSPT 787
Query: 295 AAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLRERRRK 149
+ ++ + + L ++ L+KRN K + RK
Sbjct: 788 PQITVKQNTTKSSSAPRMGMLEQLKQE--YLTKRNFESKLKSSAVSSRK 834
>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 28.3 bits (60), Expect = 1.2
Identities = 19/90 (21%), Positives = 43/90 (47%), Gaps = 2/90 (2%)
Frame = -3
Query: 409 KSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKA-- 236
+S + + +K+ IR R+L+P ++ + N Y+ + + ++ R R
Sbjct: 659 RSRSVTPINNINHKKYIRKDRELSPRGRERSSNR-NSYSDLSRSSSLSRGRSRSYTPEGR 717
Query: 235 LADAEKSGLKLSKRNPAMKAEKLRERRRKN 146
L ++E G + +PA + + R+R R++
Sbjct: 718 LIESEDKGYRSRSSSPASRKYRSRQRYRRS 747
>SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex
subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 500
Score = 25.4 bits (53), Expect = 8.2
Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 1/93 (1%)
Frame = -3
Query: 427 DLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAM-LKLNPYAAVLKRKAILELRRR 251
++T LK DE+RK+ N + L + +++ + L+ + K + +EL
Sbjct: 366 EVTETLKGDELRKISLQVNVKFSEEEVTLEDVDDDEIEDILLDKDEILTKTQVWMEL--- 422
Query: 250 KNLKALADAEKSGLKLSKRNPAMKAEKLRERRR 152
N LA+ E LKL + + R+RRR
Sbjct: 423 -NKDYLAEEEAKNLKLQEDLKKGIVRQPRKRRR 454
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 25.4 bits (53), Expect = 8.2
Identities = 17/54 (31%), Positives = 32/54 (59%)
Frame = +2
Query: 293 RVRIEFQHRLVVSERVQFACSTDHAFVGSTEDLPDLIRLEKTCEVSVGHLRLGQ 454
+V +EF+ RL + +RV+ AF+GS E + L+ + +T + ++ L LG+
Sbjct: 207 QVAVEFRKRLNIGDRVKDGLLYKDAFLGS-EAVDVLMHIVRTTDRNLA-LLLGR 258
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,266,810
Number of Sequences: 5004
Number of extensions: 40876
Number of successful extensions: 133
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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