BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P28_pT_E02
(614 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholi... 26 0.34
DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholi... 25 0.44
AY280848-1|AAQ16312.1| 632|Apis mellifera hyperpolarization-act... 25 0.78
AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic ac... 23 2.4
DQ000307-1|AAY21180.1| 423|Apis mellifera major royal jelly pro... 21 9.6
>DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholine
receptor alpha1subunit protein.
Length = 601
Score = 25.8 bits (54), Expect = 0.34
Identities = 22/75 (29%), Positives = 39/75 (52%), Gaps = 6/75 (8%)
Frame = +2
Query: 146 PYIDSYW*IQIV-K*ILSTRNLFM-CLGLYYTSILVFFLLI----KKPLLISVYLCYKLY 307
PY D + I + K + T NL + C+G+ + S+LVF+L K L IS+ L ++
Sbjct: 222 PYPDIVFNITLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKVSLSISILLSLTVF 281
Query: 308 DIINYNIIELSQINI 352
++ II + + +
Sbjct: 282 FLLLAEIIPPTSLTV 296
>DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholine
receptor alpha3subunit protein.
Length = 566
Score = 25.4 bits (53), Expect = 0.44
Identities = 21/68 (30%), Positives = 37/68 (54%), Gaps = 6/68 (8%)
Frame = +2
Query: 146 PYIDSYW*IQIV-K*ILSTRNLFM-CLGLYYTSILVFFLLI----KKPLLISVYLCYKLY 307
PY+D + I + K + T NL + C+G+ + ++LVF+L K L IS+ L ++
Sbjct: 226 PYLDITFNITMRRKTLFYTVNLIIPCMGISFLTVLVFYLPSDSGEKVSLSISILLSLTVF 285
Query: 308 DIINYNII 331
++ II
Sbjct: 286 FLLLAEII 293
>AY280848-1|AAQ16312.1| 632|Apis mellifera
hyperpolarization-activated ion channel protein.
Length = 632
Score = 24.6 bits (51), Expect = 0.78
Identities = 11/38 (28%), Positives = 22/38 (57%)
Frame = +2
Query: 326 IIELSQINILVADPGNVFFFSKGKIFNRDFSSICL*LI 439
++ LS++ V+ V+F + +F R F+ IC+ L+
Sbjct: 219 LLRLSRLVRYVSQWEEVYFLNMASVFMRIFNLICMMLL 256
>AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic
acetylcholine receptoralpha-3 protein.
Length = 537
Score = 23.0 bits (47), Expect = 2.4
Identities = 14/39 (35%), Positives = 24/39 (61%), Gaps = 2/39 (5%)
Frame = +2
Query: 146 PYIDSYW*IQIV-K*ILSTRNLFM-CLGLYYTSILVFFL 256
PY D + I + K + T NL + C+G+ + ++LVF+L
Sbjct: 235 PYSDITFNITMRRKTLFYTVNLIIPCVGITFLTVLVFYL 273
>DQ000307-1|AAY21180.1| 423|Apis mellifera major royal jelly
protein 9 protein.
Length = 423
Score = 21.0 bits (42), Expect = 9.6
Identities = 8/39 (20%), Positives = 19/39 (48%)
Frame = +1
Query: 7 YNTTHPFKHMSINLSCISNHFAEIIPNKRXVLETNNNIW 123
+N P K +I + +N + I + + + ++NI+
Sbjct: 327 WNENRPLKRRNIEIVAKNNDTLQFISGIKIIKQISSNIY 365
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 164,741
Number of Sequences: 438
Number of extensions: 3786
Number of successful extensions: 5
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 18215697
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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