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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P28_pT_E02
         (614 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ026031-1|AAY87890.1|  601|Apis mellifera nicotinic acetylcholi...    26   0.34 
DQ026032-1|AAY87891.1|  566|Apis mellifera nicotinic acetylcholi...    25   0.44 
AY280848-1|AAQ16312.1|  632|Apis mellifera hyperpolarization-act...    25   0.78 
AF514804-1|AAM51823.1|  537|Apis mellifera neuronal nicotinic ac...    23   2.4  
DQ000307-1|AAY21180.1|  423|Apis mellifera major royal jelly pro...    21   9.6  

>DQ026031-1|AAY87890.1|  601|Apis mellifera nicotinic acetylcholine
           receptor alpha1subunit protein.
          Length = 601

 Score = 25.8 bits (54), Expect = 0.34
 Identities = 22/75 (29%), Positives = 39/75 (52%), Gaps = 6/75 (8%)
 Frame = +2

Query: 146 PYIDSYW*IQIV-K*ILSTRNLFM-CLGLYYTSILVFFLLI----KKPLLISVYLCYKLY 307
           PY D  + I +  K +  T NL + C+G+ + S+LVF+L      K  L IS+ L   ++
Sbjct: 222 PYPDIVFNITLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKVSLSISILLSLTVF 281

Query: 308 DIINYNIIELSQINI 352
            ++   II  + + +
Sbjct: 282 FLLLAEIIPPTSLTV 296


>DQ026032-1|AAY87891.1|  566|Apis mellifera nicotinic acetylcholine
           receptor alpha3subunit protein.
          Length = 566

 Score = 25.4 bits (53), Expect = 0.44
 Identities = 21/68 (30%), Positives = 37/68 (54%), Gaps = 6/68 (8%)
 Frame = +2

Query: 146 PYIDSYW*IQIV-K*ILSTRNLFM-CLGLYYTSILVFFLLI----KKPLLISVYLCYKLY 307
           PY+D  + I +  K +  T NL + C+G+ + ++LVF+L      K  L IS+ L   ++
Sbjct: 226 PYLDITFNITMRRKTLFYTVNLIIPCMGISFLTVLVFYLPSDSGEKVSLSISILLSLTVF 285

Query: 308 DIINYNII 331
            ++   II
Sbjct: 286 FLLLAEII 293


>AY280848-1|AAQ16312.1|  632|Apis mellifera
           hyperpolarization-activated ion channel protein.
          Length = 632

 Score = 24.6 bits (51), Expect = 0.78
 Identities = 11/38 (28%), Positives = 22/38 (57%)
 Frame = +2

Query: 326 IIELSQINILVADPGNVFFFSKGKIFNRDFSSICL*LI 439
           ++ LS++   V+    V+F +   +F R F+ IC+ L+
Sbjct: 219 LLRLSRLVRYVSQWEEVYFLNMASVFMRIFNLICMMLL 256


>AF514804-1|AAM51823.1|  537|Apis mellifera neuronal nicotinic
           acetylcholine receptoralpha-3 protein.
          Length = 537

 Score = 23.0 bits (47), Expect = 2.4
 Identities = 14/39 (35%), Positives = 24/39 (61%), Gaps = 2/39 (5%)
 Frame = +2

Query: 146 PYIDSYW*IQIV-K*ILSTRNLFM-CLGLYYTSILVFFL 256
           PY D  + I +  K +  T NL + C+G+ + ++LVF+L
Sbjct: 235 PYSDITFNITMRRKTLFYTVNLIIPCVGITFLTVLVFYL 273


>DQ000307-1|AAY21180.1|  423|Apis mellifera major royal jelly
           protein 9 protein.
          Length = 423

 Score = 21.0 bits (42), Expect = 9.6
 Identities = 8/39 (20%), Positives = 19/39 (48%)
 Frame = +1

Query: 7   YNTTHPFKHMSINLSCISNHFAEIIPNKRXVLETNNNIW 123
           +N   P K  +I +   +N   + I   + + + ++NI+
Sbjct: 327 WNENRPLKRRNIEIVAKNNDTLQFISGIKIIKQISSNIY 365


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 164,741
Number of Sequences: 438
Number of extensions: 3786
Number of successful extensions: 5
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 18215697
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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