Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P28_pT_C23
(631 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 31 0.18
SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccha... 31 0.18
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 29 0.55
SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 29 0.55
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 28 0.97
SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|... 28 1.3
SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual 28 1.3
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 27 1.7
SPAC4F8.04 |||Brix domain protein Rpf1|Schizosaccharomyces pombe... 27 1.7
SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomy... 27 3.0
SPAC4F8.02c |mrpl40|SPAC644.02|mitochondrial ribosomal protein s... 26 3.9
SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccha... 26 3.9
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 5.2
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 25 6.8
SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po... 25 6.8
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 25 9.0
SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|ch... 25 9.0
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 25 9.0
>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1327
Score = 30.7 bits (66), Expect = 0.18
Identities = 15/65 (23%), Positives = 31/65 (47%)
Frame = -3
Query: 473 PTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQKV 294
PT KK + ++ K + ++ +E N + VK + L+ ++EK P W + +
Sbjct: 531 PTAKKNNSFKPKLFRRSRELSEKLCNNWSERVKSPSYQLKVREREKLPAWESRRKIMDAI 590
Query: 293 KEEEV 279
+ +V
Sbjct: 591 QHSQV 595
>SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 578
Score = 30.7 bits (66), Expect = 0.18
Identities = 17/70 (24%), Positives = 36/70 (51%)
Frame = -3
Query: 485 KFVKPTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPG 306
K K +++++ + ++ A +++K + ++ KK+ ++D+ K D K K G
Sbjct: 49 KMAKRSVEELKRSADEEASVKRKEKKSKHEHKKH---KKDKPSADKDRISKKDKKKSKKG 105
Query: 305 DQKVKEEEVE 276
K KEE +E
Sbjct: 106 KSKTKEESIE 115
>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1253
Score = 29.1 bits (62), Expect = 0.55
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = -3
Query: 404 NFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQ 300
+FRNQ+K ++ L E+ KE W +G DQ
Sbjct: 1070 DFRNQIKSQTERILVLREKKKEDPISWMEGTMEDQ 1104
>SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 814
Score = 29.1 bits (62), Expect = 0.55
Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 2/59 (3%)
Frame = -3
Query: 446 ENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQK--VKEEEVE 276
+N F K E NF + +K K +FT E+K + K P +QK +K+++ E
Sbjct: 254 DNFFRDFLKVCDECNFNHNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDE 312
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 28.3 bits (60), Expect = 0.97
Identities = 25/97 (25%), Positives = 49/97 (50%), Gaps = 6/97 (6%)
Frame = -3
Query: 551 HEVRKRDY-----EISDLNSQVNDLRGKFVKPTLKKVS-KYENKFAKLQKKAAEFNFRNQ 390
+E KRDY E +L QVN+L+G V P S ++ N ++ KA ++Q
Sbjct: 1414 YENLKRDYDRLNLEKHNLEKQVNELKGAEVSPQPTGQSLQHVNLAHAIELKA----LKDQ 1469
Query: 389 LKVVKKKEFTLEEEDKEKKPDWSKGKPGDQKVKEEEV 279
+ K K F+++ + ++++ + K +KV ++ +
Sbjct: 1470 INSEKAKMFSVQVQYEKREQELQKRIASLEKVNKDSL 1506
>SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1402
Score = 27.9 bits (59), Expect = 1.3
Identities = 17/85 (20%), Positives = 40/85 (47%), Gaps = 1/85 (1%)
Frame = -3
Query: 599 YYDRM-YVCEGQKWDLXHEVRKRDYEISDLNSQVNDLRGKFVKPTLKKVSKYENKFAKLQ 423
YY ++ Y C + W++ ++ + +Y SD + +F K T+ V+ Y F +
Sbjct: 541 YYKKLKYACTPKSWNISDDLGQVEYIFSDKTGTLTQNVMEFKKCTINGVA-YGEAFTEAM 599
Query: 422 KKAAEFNFRNQLKVVKKKEFTLEEE 348
A+ ++ ++ +K+ +E +
Sbjct: 600 AGMAKREGKDTEELTLQKQSFIERD 624
>SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 27.9 bits (59), Expect = 1.3
Identities = 16/71 (22%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = -3
Query: 506 QVNDLRGKFVKPTLKKVSK-YENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKP 330
Q N +RG+ + + K+ K Y+N+ ++K + +++ + + + P
Sbjct: 418 QGNFVRGQKLHLEISKIQKKYQNQIENMKKGSHVTKSNQYSEMIGNLPYPTSSRKRTRSP 477
Query: 329 DWSKGKPGDQK 297
SKGK D+K
Sbjct: 478 LMSKGKSYDRK 488
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 27.5 bits (58), Expect = 1.7
Identities = 25/121 (20%), Positives = 58/121 (47%), Gaps = 7/121 (5%)
Frame = -3
Query: 617 KRIIQEYYDRMYVCEGQKWDLXHEV-----RKRDYEISDLNSQVNDLRGKFVKPTLKKVS 453
+R++Q Y ++ V + ++ L E+ RK++ E+ + + K K+
Sbjct: 554 QRVLQAYREK--VAQQRQAKLLEEIEEENKRKQERELKKIREKEKKRDKKKQLKLAKEEE 611
Query: 452 KYENKFAKLQKKAAE--FNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQKVKEEEV 279
+ + +L ++AA+ + Q + KK+E + ++EKK + + ++K K++E
Sbjct: 612 RQRREAERLAEQAAQKALEAKRQEEARKKREEQRLKREQEKKQQELERQKREEKQKQKER 671
Query: 278 E 276
E
Sbjct: 672 E 672
>SPAC4F8.04 |||Brix domain protein Rpf1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 306
Score = 27.5 bits (58), Expect = 1.7
Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
Frame = -3
Query: 458 VSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEE-DKEKK 333
+ K +NK + Q+ + +N+ K+ ++KE EEE D EKK
Sbjct: 1 MGKIKNKIVRQQQYMKALHQKNKDKLERRKERAKEEEKDPEKK 43
>SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 327
Score = 26.6 bits (56), Expect = 3.0
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = -3
Query: 380 VKKKEFTLEEEDKEKKPDWSKGKPGDQKVKEEEVE 276
++ E+ L++E+KE+K K + +K KE+E E
Sbjct: 275 IESGEYFLKKEEKERKKRAEKKEQQKEKKKEKEKE 309
>SPAC4F8.02c |mrpl40|SPAC644.02|mitochondrial ribosomal protein
subunit L40|Schizosaccharomyces pombe|chr 1|||Manual
Length = 279
Score = 26.2 bits (55), Expect = 3.9
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = -3
Query: 422 KKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQ 300
KK + R +++ K ++DK KP+ GKP D+
Sbjct: 22 KKPLPLHMRTKIREHLNKSDPTVKDDKSAKPELPFGKPADR 62
>SPCC364.06 |nap1||nucleosome assembly protein Nap1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 393
Score = 26.2 bits (55), Expect = 3.9
Identities = 24/96 (25%), Positives = 43/96 (44%), Gaps = 12/96 (12%)
Frame = -3
Query: 560 DLXHEVRKRDYEISDLNSQVNDLRGKFVKPTLKKVSKYENKFAKLQKKAAEF----NFRN 393
+L V++R + L + +DL +F K + Y K+A + K+ +E +
Sbjct: 70 ELPEAVQRRISGLRGLQKRYSDLESQFQKELFELEKAYAKKYAPIFKRRSEVVRGADEPT 129
Query: 392 QLKVVK--------KKEFTLEEEDKEKKPDWSKGKP 309
+ ++ K KKE T E K++ D +KG P
Sbjct: 130 EEEIKKGEAADENEKKEPTSSESKKQEGGDDTKGIP 165
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 25.8 bits (54), Expect = 5.2
Identities = 15/61 (24%), Positives = 31/61 (50%), Gaps = 1/61 (1%)
Frame = -3
Query: 488 GKFVKPTLKK-VSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGK 312
G+ + P L + + +E++F Q+ AE+ + Q + + + TLE + + W +G
Sbjct: 1385 GEHLIPNLYRYIQPWESEFIDSQRVWAEYAMKRQEALQQNRRLTLE----DLEDSWDRGI 1440
Query: 311 P 309
P
Sbjct: 1441 P 1441
>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 865
Score = 25.4 bits (53), Expect = 6.8
Identities = 19/63 (30%), Positives = 29/63 (46%), Gaps = 4/63 (6%)
Frame = -2
Query: 588 HVCV*GPEVGF-GT*SQEKRL*DLRPELPSQRPQRQIRQAHT---KEGFQIRKQIRQAPE 421
H+ G E GF + ++ L+ + + R + + HT KE QIRK IRQ
Sbjct: 10 HLTCSGVERGFTNSPKTHPKMSSLKSFIKAVRASKTTAEEHTTILKESAQIRKNIRQGSN 69
Query: 420 EGR 412
+ R
Sbjct: 70 DMR 72
>SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 284
Score = 25.4 bits (53), Expect = 6.8
Identities = 30/106 (28%), Positives = 43/106 (40%), Gaps = 4/106 (3%)
Frame = -3
Query: 593 DRMYVCEGQKWDLXHEVRKRDYEISDLNSQVNDLRGKFVKPTLKKVSKYENKFAKLQKKA 414
D VCE + KR S S+ G + P KK+ K K K KK
Sbjct: 149 DEDRVCEDASSSDEAKREKRKKHSSKKKSKKKTSTGSALDP--KKLEKITKKKKKEHKKK 206
Query: 413 AEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGD----QKVKE 288
+ + K+K + ++E+K+KK K KP +KVKE
Sbjct: 207 DK-----ESSSKKRKSGSSDKEEKKKKKIKLKDKPESTSSVEKVKE 247
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 25.0 bits (52), Expect = 9.0
Identities = 21/68 (30%), Positives = 33/68 (48%)
Frame = -3
Query: 485 KFVKPTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPG 306
K +P++ K K E K K ++A+ +K V+K LE KEK+ K
Sbjct: 206 KIQRPSVIKKDKKEKKEGKPSQEAS-------VKSVEKAPKGLEGAKKEKQNKKEKKDKK 258
Query: 305 DQKVKEEE 282
D+K K+E+
Sbjct: 259 DKKDKKEK 266
>SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 333
Score = 25.0 bits (52), Expect = 9.0
Identities = 15/72 (20%), Positives = 32/72 (44%)
Frame = -3
Query: 542 RKRDYEISDLNSQVNDLRGKFVKPTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEF 363
+K Y + V D+ ++++ + + + LQ+ E N + ++ +
Sbjct: 225 KKMSYCLGFGIDSVRDVSHRYIRHPENGLPRDRCPESVLQQALHEINIEFRSRLTDSERK 284
Query: 362 TLEEEDKEKKPD 327
LEEEDK +K +
Sbjct: 285 ALEEEDKREKDE 296
>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
Upf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 925
Score = 25.0 bits (52), Expect = 9.0
Identities = 11/37 (29%), Positives = 21/37 (56%), Gaps = 3/37 (8%)
Frame = -3
Query: 569 QKWDLXHEVRKRDY---EISDLNSQVNDLRGKFVKPT 468
++W++ + ++R+ + D SQV D KF +PT
Sbjct: 887 EEWNVFAQYKRRESNATDFEDFRSQVGDDESKFDEPT 923
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,378,195
Number of Sequences: 5004
Number of extensions: 45316
Number of successful extensions: 149
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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