Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P28_F_L12
(933 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_32263| Best HMM Match : Ldh_1_C (HMM E-Value=8.80015e-42) 270 1e-72
SB_38683| Best HMM Match : Ldh_1_N (HMM E-Value=1.7e-11) 181 8e-46
SB_48488| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.14
SB_44832| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.44
SB_44638| Best HMM Match : TFIIB (HMM E-Value=0.0079) 31 1.0
SB_37178| Best HMM Match : fn3 (HMM E-Value=4.7e-08) 31 1.3
SB_28327| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.4
SB_3675| Best HMM Match : TRAP_alpha (HMM E-Value=0) 29 5.4
SB_18084| Best HMM Match : DUF801 (HMM E-Value=0.37) 28 9.4
SB_12464| Best HMM Match : Nsp1_C (HMM E-Value=0) 28 9.4
SB_10638| Best HMM Match : Myosin_N (HMM E-Value=1.5e-06) 28 9.4
SB_13186| Best HMM Match : Nsp1_C (HMM E-Value=6.8e-07) 28 9.4
>SB_32263| Best HMM Match : Ldh_1_C (HMM E-Value=8.80015e-42)
Length = 411
Score = 270 bits (661), Expect = 1e-72
Identities = 127/233 (54%), Positives = 163/233 (69%)
Frame = +1
Query: 229 IASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCALPLLAGVLPTANPEEAFKDVAA 408
I SG + P+ LHLLDI PMMG L GVV+EL DCA+PLL V+ T + E+AF D+
Sbjct: 102 IPSGVNVHIELPIILHLLDITPMMGALGGVVLELQDCAIPLLHEVVATDDAEKAFTDIDV 161
Query: 409 AFLVGAMPRKEGMERKDLLAANVRIFKEQGQALDKAARKDVKVLVVGNPANTNALICSKY 588
A LVGAMPR+EGMERKDLL+AN +IF+ QG+ALDK A+K K+LVVGNPANTN LI ++
Sbjct: 162 AILVGAMPRREGMERKDLLSANAKIFEAQGKALDKVAKKTCKILVVGNPANTNCLIAQRW 221
Query: 589 APSIPKENFTAMTRLDQNRAQSQLAAKIGVPVKDVKRVIIWGNHSSTQFPDASNAVAIVG 768
APSIPKENF+ +TRLDQNR SQ+A ++ + V VK VIIWGNHS+TQ+PD ++ V
Sbjct: 222 APSIPKENFSCLTRLDQNRGGSQIAGRLNLRVDQVKNVIIWGNHSATQYPDVNHGKVCVN 281
Query: 769 GAQKSVSEIINDDAYLKGAFVTTVQKRGAAVIXXXXXXXXXXXXXXXXXHMKN 927
G ++ + + DD YL G F++TVQKRGAA+I HM++
Sbjct: 282 GNWVAIRDAVKDDDYLNGEFISTVQKRGAAIIKARKLSSAMSAAKAICDHMRD 334
>SB_38683| Best HMM Match : Ldh_1_N (HMM E-Value=1.7e-11)
Length = 462
Score = 181 bits (440), Expect = 8e-46
Identities = 92/240 (38%), Positives = 145/240 (60%), Gaps = 10/240 (4%)
Frame = +1
Query: 157 KMAEPIRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELAD 336
K P+RV V+ A+G +AY +L + G VFG Q+ V ++LLD L+GV E+ D
Sbjct: 127 KKINPLRVCVSKASGPLAYGMLASLVQGEVFGFQEEVSIYLLDTPENQEALQGVAYEIED 186
Query: 337 CALPLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFKEQGQALDKA 516
CA PL GV T++P AFKD + L+ EG ++K+ L ++ ++F++ G+AL+
Sbjct: 187 CAWPLFRGVHITSDPAVAFKDASVVVLLDGKAINEGTDKKEYLLSHAKLFRDYGKALEAH 246
Query: 517 ARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQLAAKIGVPVKDVK 696
A+ D KVL G PAN + I SK+APSI K+NF +++R+++NRA+ +A ++ V +K
Sbjct: 247 AKPDCKVLTAGGPANFSTFIASKFAPSIQKKNFVSLSRIEENRAKGLIAKRLNVNTAGIK 306
Query: 697 RVIIWGNHSSTQFPDASNA------VAIVG----GAQKSVSEIINDDAYLKGAFVTTVQK 846
+I+WGN +PDAS+A AI G G + V E+++D+ +L G FV ++K
Sbjct: 307 DLIVWGNPGFNHYPDASHARVDGFDGAIWGPHVPGFTRPVPEMVHDNKWLSGEFVEVLKK 366
>SB_48488| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 391
Score = 34.3 bits (75), Expect = 0.14
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = -1
Query: 354 QKWQSTVGQLHDNTFKYTHHRRNIKKVEEDRLLRS 250
+ W T G +H+ FKY H RR ++ +E++ L R+
Sbjct: 332 RSWLPTWGAIHEELFKYHHKRRFLRDLEDELLPRT 366
>SB_44832| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 292
Score = 32.7 bits (71), Expect = 0.44
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -1
Query: 348 WQSTVGQLHDNTFKYTHHRRNIKKVEEDRL 259
W T G +H+ FKY H RR ++ +E++ L
Sbjct: 235 WLPTWGAIHEELFKYHHKRRFLRDLEDELL 264
>SB_44638| Best HMM Match : TFIIB (HMM E-Value=0.0079)
Length = 472
Score = 31.5 bits (68), Expect = 1.0
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -1
Query: 354 QKWQSTVGQLHDNTFKYTHHRRNIKKVEEDRL 259
+ W T +HD+ F++ H RR IK +E++ L
Sbjct: 152 RSWLPTWSAVHDDLFRHHHKRRFIKSLEDELL 183
>SB_37178| Best HMM Match : fn3 (HMM E-Value=4.7e-08)
Length = 961
Score = 31.1 bits (67), Expect = 1.3
Identities = 16/51 (31%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Frame = -1
Query: 366 KNPSQKWQSTVGQLHDNTFKYTHHRRNIKKVEEDRL--LRSKNCSRRNLIK 220
+N +KW+S++ Q D +Y RRN + E++L LR ++ N++K
Sbjct: 227 ENNRKKWESSMQQRRDKEVEYMEARRNRVEDYENQLQTLRIQDAEEYNMVK 277
>SB_28327| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 576
Score = 29.1 bits (62), Expect = 5.4
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -1
Query: 552 VANNKDLHIFTSRFVQSLALLFEDAHISSKKIL 454
+ N KDLHI+TS ++ L + E A K IL
Sbjct: 131 LVNEKDLHIWTSTLLRVLRVKIEQAEQQKKGIL 163
>SB_3675| Best HMM Match : TRAP_alpha (HMM E-Value=0)
Length = 667
Score = 29.1 bits (62), Expect = 5.4
Identities = 13/23 (56%), Positives = 14/23 (60%), Gaps = 3/23 (13%)
Frame = -3
Query: 556 LGCQQQGPSH---LYEPLCPKPG 497
LGC QGP H L+E CP PG
Sbjct: 482 LGCPWQGPHHELALHEGACPHPG 504
>SB_18084| Best HMM Match : DUF801 (HMM E-Value=0.37)
Length = 599
Score = 28.3 bits (60), Expect = 9.4
Identities = 24/78 (30%), Positives = 37/78 (47%), Gaps = 6/78 (7%)
Frame = -2
Query: 518 AALSKAWP--CSLKMRTLAARRSFLSIPSFLGIAPTRKAAATSLKASSGFAVGRTP---- 357
+ LSKA P CS M + S S+P+ A A+A+S SS ++ +
Sbjct: 312 SGLSKAGPSNCSCDMNASPSALSASSLPASASSAIASSASASSASMSSASSLSASASSNI 371
Query: 356 AKSGRAQSANSMTTPSST 303
A S A SAN+ + +S+
Sbjct: 372 ASSDIASSANASSAKTSS 389
>SB_12464| Best HMM Match : Nsp1_C (HMM E-Value=0)
Length = 467
Score = 28.3 bits (60), Expect = 9.4
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = -2
Query: 449 SIPSFLGIAPTRKAAATSLKASSGFAVGRT-PAKSGRAQSANSMTTPSSTPIIG 291
++ FL APT AT SGF G P +G + + + PS+TP G
Sbjct: 73 TLAPFLTAAPTG-LTATPAATGSGFQFGTAAPGAAGVSFKLGTASAPSTTPATG 125
>SB_10638| Best HMM Match : Myosin_N (HMM E-Value=1.5e-06)
Length = 1977
Score = 28.3 bits (60), Expect = 9.4
Identities = 22/76 (28%), Positives = 35/76 (46%), Gaps = 2/76 (2%)
Frame = -2
Query: 515 ALSKAWPCSLKMRTLAARRSFLSIPSFLGIAPTRKAAATSLKASSGFAVGRTPAKS--GR 342
ALS P +L+ + + S+ P RK ATSL+ + G T KS G
Sbjct: 905 ALSGLAPTNLESHSTPTQPSYYRSWYSGSFFPNRKDNATSLQEGQLVSTGNTHTKSKVGS 964
Query: 341 AQSANSMTTPSSTPII 294
A++ + ++ S +P I
Sbjct: 965 AKAEKAPSSDSKSPYI 980
>SB_13186| Best HMM Match : Nsp1_C (HMM E-Value=6.8e-07)
Length = 329
Score = 28.3 bits (60), Expect = 9.4
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = -2
Query: 449 SIPSFLGIAPTRKAAATSLKASSGFAVGRT-PAKSGRAQSANSMTTPSSTPIIG 291
++ FL APT AT SGF G P +G + + + PS+TP G
Sbjct: 73 TLAPFLTAAPTG-LTATPAATGSGFQFGTAAPGAAGVSFKLGTASAPSTTPATG 125
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 26,425,855
Number of Sequences: 59808
Number of extensions: 542479
Number of successful extensions: 1585
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1501
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1584
length of database: 16,821,457
effective HSP length: 82
effective length of database: 11,917,201
effective search space used: 2717121828
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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