Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P28_F_H04
(889 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC36.10 |||mitochondrial intermembrane space protein sorting p... 121 1e-28
SPAP8A3.10 |||mitochondrial intermembrane space protein sorting ... 71 2e-13
SPBC21.02 |||TLDc domain protein 2|Schizosaccharomyces pombe|chr... 29 0.67
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 29 0.67
SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 29 1.2
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 28 2.0
SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 2.7
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 27 3.6
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 3.6
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 27 3.6
SPAC22E12.14c |sck2||serine/threonine protein kinase Sck2|Schizo... 27 4.7
SPBC1289.13c |||alpha-1,2-galactosyltransferase|Schizosaccharomy... 26 8.2
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 26 8.2
SPAC21E11.07 ||SPAC2C4.01|glycine cleavage T-protein|Schizosacch... 26 8.2
>SPBC36.10 |||mitochondrial intermembrane space protein sorting
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 121 bits (292), Expect = 1e-28
Identities = 68/172 (39%), Positives = 98/172 (56%), Gaps = 3/172 (1%)
Frame = +2
Query: 71 MKIWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIGTDVVERKVVD-GVLHTHRLVSSKWF 247
MKI+ S H F +P+E V+ A W+KYPN VI D ++RKV+D GVL+T RL++
Sbjct: 1 MKIFESCHLFQYPFEQVSAAHWQKYPNEHATHVIAVDTLDRKVLDNGVLYTERLITCHQA 60
Query: 248 FPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHYIAVDETVRYTPHPSDSSK 427
PRW LI A+ CY E S V+ R +TL T+NLTF + VDETV Y+PHP +
Sbjct: 61 LPRWILKLIDGAQDCYIRETSYVDLKARTLTLLTSNLTFSDRLRVDETVTYSPHP-ELEA 119
Query: 428 TLLKQEAVVTVQGV--PLSSYMEDLLTNKISLNAGKGRQAIEWVIGKFDTEI 577
T+ +QEA + LS+ +E + A +G++ E V+ K + +
Sbjct: 120 TVFQQEARIEALACMKRLSNLIEQWSVDGFGKKASRGKEGFESVLEKINMSV 171
>SPAP8A3.10 |||mitochondrial intermembrane space protein sorting
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 171
Score = 70.9 bits (166), Expect = 2e-13
Identities = 42/121 (34%), Positives = 64/121 (52%), Gaps = 1/121 (0%)
Frame = +2
Query: 77 IWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIGTDVVERKVVD-GVLHTHRLVSSKWFFP 253
I T + N W TV+ A +YPNP + V+ DV+ER V D G L+T RL+ + P
Sbjct: 4 ICTDKTELNASWNTVSSAWLTRYPNPYSLHVVSADVLERYVDDEGRLYTERLLVKQGRLP 63
Query: 254 RWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHYIAVDETVRYTPHPSDSSKTL 433
RWA L+ K Y E S ++P ++++ +T NL + V E R+ + SKT+
Sbjct: 64 RWASDLLNVNK-SYILERSVIDPSKQELKSETFNLDHVKILRVIEYSRFIQSSENCSKTI 122
Query: 434 L 436
+
Sbjct: 123 V 123
>SPBC21.02 |||TLDc domain protein 2|Schizosaccharomyces pombe|chr
2|||Manual
Length = 511
Score = 29.5 bits (63), Expect = 0.67
Identities = 27/120 (22%), Positives = 49/120 (40%)
Frame = +2
Query: 14 SLRRSLSLYCNLH*ILEKNMKIWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIGTDVVER 193
+L R + Y N +L K KI + + + P A RKYP P IGTD
Sbjct: 313 ALERKMCNYHNPSILLIKAKKINANHKSSSRPISLDATIP-RKYP----PHCIGTDKAVP 367
Query: 194 KVVDGVLHTHRLVSSKWFFPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHY 373
+ H ++ + RW Q+ +G +++ + ++ PI + + +C +
Sbjct: 368 QKFGADFHNENILLGAYISTRWRQSHMGFFG-DHSTLLFQLQPIHQVYYASNLDKNYCMF 426
>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1115
Score = 29.5 bits (63), Expect = 0.67
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = +2
Query: 578 KELASSACKSTDELLSQTKKSIDDITTSARRSMDDISSKAKSTLDDIEKLTK 733
K L + +S ++ LSQ K ++ +TTS D +S+ + D+++ L K
Sbjct: 689 KLLLNEQIESLNDQLSQLKTEMESVTTSKESLADYLSNLKERHNDELDSLNK 740
>SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit
Apc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1458
Score = 28.7 bits (61), Expect = 1.2
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = +2
Query: 497 LTNKISLNAGKGRQAIEWVIGKFDTEIKELASSACKSTDELLSQTK 634
L N++ +++ + +WV K D E+KE+ + TD L T+
Sbjct: 641 LVNRLDVDSFLHPKTPKWVFNKQDQEVKEIKALTSTVTDSTLVDTQ 686
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 27.9 bits (59), Expect = 2.0
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = +2
Query: 620 LSQTKKSIDDITTSARRSMDDISSKAKS 703
L KKS DDITT +R M+D S +S
Sbjct: 177 LCSPKKSKDDITTPKKRLMEDTYSPRES 204
>SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 525
Score = 27.5 bits (58), Expect = 2.7
Identities = 16/62 (25%), Positives = 26/62 (41%)
Frame = +2
Query: 104 HPWETVAQAAWRKYPNPMNPAVIGTDVVERKVVDGVLHTHRLVSSKWFFPRWAQALIGTA 283
H W+ + YPN AV ++ G +H L+ S+ WA A++G A
Sbjct: 431 HIWQAMLLYVTDAYPNVSASAVAAFELPSNLGAVGFIHLSALMFSR-MNVHWATAVVGFA 489
Query: 284 KI 289
+
Sbjct: 490 SL 491
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 27.1 bits (57), Expect = 3.6
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = -2
Query: 708 SVLFALDEISSIDLLADVVMSSIDFFVCDNNSSVLLQAELA 586
+++ L I LL +++ S+ CDNN + +LQ +A
Sbjct: 554 NIIDKLTSNEQISLLLKIIIPSLTTLACDNNGTHVLQKCIA 594
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.1 bits (57), Expect = 3.6
Identities = 30/116 (25%), Positives = 46/116 (39%)
Frame = -2
Query: 720 SISSSVLFALDEISSIDLLADVVMSSIDFFVCDNNSSVLLQAELASSLISVSNFPITHSI 541
SISS+V + SS A S+ N SS+L + +S+ +S +N S
Sbjct: 244 SISSTVSSSTPLTSSNSTTAATSASATSSSAQYNTSSLLPSSTPSSTPLSSANSTTATSA 303
Query: 540 ACLPLPAFREILLVKRSSM*LLRGTPCTVTTASCFSNVFEESEGCGVYRTVSSTAM 373
+ PL + SS L + TTA+ S+ S + SST +
Sbjct: 304 SSTPLTSVNSTTTTSASSTPLSSVSSANSTTATSTSSTPLSSVNSTTATSASSTPL 359
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 27.1 bits (57), Expect = 3.6
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -3
Query: 389 FHQQQCN-DRMLGLLFLKSSVAVLGLLLISH*HN 291
FH Q N D++L + FL V ++G+L +H HN
Sbjct: 443 FHPPQMNTDQLLLVSFLGLVVNLVGILAFNHGHN 476
>SPAC22E12.14c |sck2||serine/threonine protein kinase
Sck2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 646
Score = 26.6 bits (56), Expect = 4.7
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = +2
Query: 101 NHPWETVAQAAWRKYPNPMNPAVIGTDVVERKVVDGVLHTH 223
+HP T A PNP+ P+ + DV + +D ++ H
Sbjct: 165 SHPIPTPAIQRTSSIPNPLTPSYVVFDVAKPVPIDVNIYDH 205
>SPBC1289.13c |||alpha-1,2-galactosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 375
Score = 25.8 bits (54), Expect = 8.2
Identities = 14/55 (25%), Positives = 22/55 (40%)
Frame = +2
Query: 344 KTTNLTFCHYIAVDETVRYTPHPSDSSKTLLKQEAVVTVQGVPLSSYMEDLLTNK 508
+TT +T E PHP +S LL PL+ Y+ ++ N+
Sbjct: 77 ETTTVTPTVTATAQELSTLNPHPENSKIVLLMGSNAQNDPSSPLNPYIRTIIKNR 131
>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/55 (23%), Positives = 29/55 (52%)
Frame = +2
Query: 191 RKVVDGVLHTHRLVSSKWFFPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTN 355
RK+++ +LH+H +SS +F+ + + A + + +NP R + ++N
Sbjct: 42 RKIINEMLHSHSELSS-FFYAEKSNTFLTIAGLAESELFDTLNPAFRISSCPSSN 95
>SPAC21E11.07 ||SPAC2C4.01|glycine cleavage
T-protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 325
Score = 25.8 bits (54), Expect = 8.2
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +2
Query: 422 SKTLLKQEAVVTVQGVPLSSYMEDLLTNKISLN 520
S L ++++ V+GV +++ L TNKI+L+
Sbjct: 22 SVCLNSSKSLIRVEGVDAVKFLQGLTTNKITLD 54
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,764,512
Number of Sequences: 5004
Number of extensions: 80846
Number of successful extensions: 226
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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