BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P28_F_G04
(558 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_18026| Best HMM Match : No HMM Matches (HMM E-Value=.) 40 0.001
SB_18023| Best HMM Match : No HMM Matches (HMM E-Value=.) 39 0.003
SB_46131| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.48
SB_17384| Best HMM Match : zf-C2H2 (HMM E-Value=0) 29 3.4
SB_14229| Best HMM Match : VAR1 (HMM E-Value=5) 28 4.5
>SB_18026| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 621
Score = 39.9 bits (89), Expect = 0.001
Identities = 17/43 (39%), Positives = 28/43 (65%)
Frame = +1
Query: 178 MSVIIRLQNLPWSANALDIRNFFRGLSIPEGGVHIVGGELGDA 306
M V+I+++ LP+ A + DI+ FF GLS+ E +H+ + G A
Sbjct: 1 MPVVIKMKGLPFEATSRDIQMFFDGLSLREKDIHLAANKDGKA 43
Score = 28.3 bits (60), Expect = 4.5
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +1
Query: 166 EETTMSVIIRLQNLPWSANALDIRNFFRGLSI 261
++ S I+R+ LP+ ++ D+ FF GL I
Sbjct: 327 DQEATSTIVRMFGLPFESSKRDLYKFFNGLKI 358
Score = 27.9 bits (59), Expect = 5.9
Identities = 18/48 (37%), Positives = 27/48 (56%), Gaps = 4/48 (8%)
Frame = +1
Query: 187 IIRLQNLPWSANALDIRNFFRGLSI-PEG-GVHIV--GGELGDAFIAF 318
++ ++NLP S A I +FF G ++ PE +H + G GDA I F
Sbjct: 546 VVGIRNLPSSITADQILDFFYGFNVLPETIKIHYLSPGRSSGDAMITF 593
>SB_18023| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 500
Score = 38.7 bits (86), Expect = 0.003
Identities = 14/26 (53%), Positives = 20/26 (76%)
Frame = +1
Query: 187 IIRLQNLPWSANALDIRNFFRGLSIP 264
++R + LPW A+ D+ NFFRGL+IP
Sbjct: 174 VLRARGLPWQASDQDVANFFRGLNIP 199
Score = 37.5 bits (83), Expect = 0.007
Identities = 24/57 (42%), Positives = 32/57 (56%), Gaps = 8/57 (14%)
Frame = +1
Query: 172 TTMSVIIRLQNLPWSANALDIRNFFRGLSIP----EGGVHIVGGE----LGDAFIAF 318
T VIIR++ LP+S A D+ FF G +P EGG+ +V G GDAF+ F
Sbjct: 320 TNAEVIIRMRGLPFSTKAADVVRFF-GDDVPVYRGEGGILMVRGRNGKATGDAFVLF 375
>SB_46131| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 899
Score = 31.5 bits (68), Expect = 0.48
Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 4/48 (8%)
Frame = +1
Query: 187 IIRLQNLPWSANALDIRNFFRGLSIPEGGVHIV----GGELGDAFIAF 318
+++L+ LP+ + +I FF GL I G+ I G G+ F+ F
Sbjct: 39 VVKLRGLPYGCSKEEIAQFFTGLEIVPNGITITLDEEGKTTGEGFVEF 86
>SB_17384| Best HMM Match : zf-C2H2 (HMM E-Value=0)
Length = 425
Score = 28.7 bits (61), Expect = 3.4
Identities = 17/52 (32%), Positives = 21/52 (40%)
Frame = +3
Query: 168 RNNHECHHKTTEPALVGKCSGY*EFLSGPFDTRGGCTHRRRRAGRCFHCFQV 323
RN HE H +P C F R TH + ++ RC HC QV
Sbjct: 360 RNKHELSHSREKPFNCKYCDR--SFSQSSTVLRHMRTHDKDKSYRCVHCGQV 409
>SB_14229| Best HMM Match : VAR1 (HMM E-Value=5)
Length = 356
Score = 28.3 bits (60), Expect = 4.5
Identities = 21/72 (29%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
Frame = -1
Query: 273 TPLGYR-KAPKEIPNIQSICRPRQVL*SYDDTHGCFFYCLYTRT*CPPSRLQNEKHCKNT 97
TP+ Y K PKE P ++ PRQ ++G F Y PP + ++ N
Sbjct: 44 TPVLYSCKTPKESPQSATLATPRQ------KSNGAFGYKKTFNQAAPPYQKALDESGYNY 97
Query: 96 TVHRKISITHRR 61
T+H + + T++R
Sbjct: 98 TLHYEPNTTNKR 109
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,645,083
Number of Sequences: 59808
Number of extensions: 266815
Number of successful extensions: 605
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 534
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 605
length of database: 16,821,457
effective HSP length: 78
effective length of database: 12,156,433
effective search space used: 1300738331
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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