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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P28_F_F21
         (801 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_36564| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   2.5  
SB_25673| Best HMM Match : MFS_1 (HMM E-Value=0.18)                    29   4.4  
SB_17493| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.8  

>SB_36564| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 422

 Score = 29.9 bits (64), Expect = 2.5
 Identities = 19/66 (28%), Positives = 31/66 (46%), Gaps = 2/66 (3%)
 Frame = -1

Query: 735 IPQNQAPFHYRNTEVATTAVLVHHSLLDSRQVTDIL*L--SLQ*EHFGLYILDLLQLPCF 562
           IP  Q P +YR+  +  T +L          +TD L L  S    H  +  + LLQ PC+
Sbjct: 243 IPLLQTPCYYRHLAITDTLLLQTPCYYRHPAITDTLLLQTSRYYRHPPITDIPLLQTPCY 302

Query: 561 HHYKSV 544
           + + ++
Sbjct: 303 YRHPAI 308



 Score = 29.5 bits (63), Expect = 3.3
 Identities = 21/70 (30%), Positives = 33/70 (47%), Gaps = 3/70 (4%)
 Frame = -1

Query: 744 PLT-IPQNQAPFHYRNTEVATTAVLVHHSLLDSRQVTDIL*LSLQ*EHFGLYILD--LLQ 574
           P+T IP  Q P +YR+  +  T +L          +TD L L     +    I D  LLQ
Sbjct: 290 PITDIPLLQTPCYYRHPAITDTLLLQTPCYYRHPAITDTLLLQTSRYYRHPAITDTLLLQ 349

Query: 573 LPCFHHYKSV 544
            PC++ + ++
Sbjct: 350 TPCYYRHLAI 359



 Score = 29.1 bits (62), Expect = 4.4
 Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 2/62 (3%)
 Frame = -1

Query: 723 QAPFHYRNTEVATTAVLVHHSLLDSRQVTDIL*LSLQ*EHFGLYILD--LLQLPCFHHYK 550
           Q P +YR+  +  T +L          +TD L L     +  L I D  LLQ PC++ + 
Sbjct: 315 QTPCYYRHPAITDTLLLQTSRYYRHPAITDTLLLQTPCYYRHLAITDTLLLQTPCYYRHP 374

Query: 549 SV 544
           ++
Sbjct: 375 TI 376


>SB_25673| Best HMM Match : MFS_1 (HMM E-Value=0.18)
          Length = 634

 Score = 29.1 bits (62), Expect = 4.4
 Identities = 14/43 (32%), Positives = 22/43 (51%)
 Frame = +3

Query: 162 ISKIKCLCRSLVSIESQKTPQSGASSDGISPPVRIQRGPTDIL 290
           ++ + CL  S    E  K P+S A S G+    + +RGP  I+
Sbjct: 469 VTAVVCLVTSSFDSEIVKRPESPARSLGLDEGTKAERGPYGIM 511


>SB_17493| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 718

 Score = 28.7 bits (61), Expect = 5.8
 Identities = 15/66 (22%), Positives = 35/66 (53%), Gaps = 7/66 (10%)
 Frame = +3

Query: 138 SNIVITRQISKIK-----CLCRSLVSIESQKTPQSGASSDGISP-PV-RIQRGPTDILQA 296
           +NI++ + + K+K      +C +   ++    P+ G  S G++P P+  +   PT+ + +
Sbjct: 216 ANILLAKSLKKVKGVSWSSVCYNCTLVKLANMPRPGNGSGGVTPTPIGTVNVFPTESVSS 275

Query: 297 LSTTVG 314
            + T+G
Sbjct: 276 FNVTIG 281


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,536,095
Number of Sequences: 59808
Number of extensions: 449962
Number of successful extensions: 1017
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 936
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1017
length of database: 16,821,457
effective HSP length: 81
effective length of database: 11,977,009
effective search space used: 2215746665
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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