Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P17_pT_N13
(690 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 174 9e-45
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 97 2e-21
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 90 3e-19
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 87 2e-18
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 87 3e-18
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 82 6e-17
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 67 3e-12
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 65 8e-12
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 62 1e-10
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 60 3e-10
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 57 3e-09
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 57 3e-09
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 33 0.029
SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex ... 29 0.48
SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 7.8
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 174 bits (424), Expect = 9e-45
Identities = 81/160 (50%), Positives = 108/160 (67%)
Frame = -1
Query: 600 MIKLFSLKQQKKDEEGSTRGGGSQKKASAAQLRITKDLNELNLPKTCNTEFPDPDDLLNF 421
M K++ LK+++ +E + G S AQ+RI KD+ +L +P T +T +PDP L
Sbjct: 1 MRKIWELKKKEAQKEKNASG------ISPAQIRIQKDVTDLEIPSTMSTSWPDPIKLNVL 54
Query: 420 KLIICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILREDWK 241
L I PDEG+Y+GGKF F ++ NYPH+PPKVKC +YHPNID+EGNVCLNILR+DW
Sbjct: 55 HLEIRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWN 114
Query: 240 PVLTVNSIVYGLQYLFLEPNPEDPLXQGSSGRAPKQQATF 121
PVL +NSI+ GLQ+LFL PN EDPL + ++ K F
Sbjct: 115 PVLNLNSILVGLQFLFLSPNAEDPLNKEAAADLHKDPQGF 154
Score = 29.1 bits (62), Expect = 0.63
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = -2
Query: 170 PSXKEAADELQSNRRLFEQHVQRAMRGGYVGTXXFERCL 54
P KEAA +L + + F V+ AM+GG V F+ +
Sbjct: 138 PLNKEAAADLHKDPQGFASRVRTAMKGGLVNGISFDNVM 176
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 97.5 bits (232), Expect = 2e-21
Identities = 46/116 (39%), Positives = 70/116 (60%), Gaps = 3/116 (2%)
Frame = -1
Query: 504 RITKDLNELNL--PKTCNTEFPDPDDLLNFKLIIC-PDEGFYRGGKFVFSFKVGPNYPHE 334
RI ++L +L P +C+ P DDL +++ I P + Y GG F S +YP +
Sbjct: 5 RINRELADLGKDPPSSCSAG-PVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFK 63
Query: 333 PPKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPL 166
PPKV T +YHPNI+ G++CL+ILR+ W P LT++ ++ + L +PNP+DPL
Sbjct: 64 PPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPL 119
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 89.8 bits (213), Expect = 3e-19
Identities = 43/115 (37%), Positives = 63/115 (54%), Gaps = 2/115 (1%)
Frame = -1
Query: 504 RITKDLNELNL-PKTCNTEFPDPDDLLNFKLII-CPDEGFYRGGKFVFSFKVGPNYPHEP 331
RI K++ L P P D+L FK+ + P + Y GGKF + YP P
Sbjct: 6 RIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMMP 65
Query: 330 PKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPL 166
P V+ T +YHPN+D G +CL+ L++DW P L + +++ +Q L PNP+DPL
Sbjct: 66 PNVRFLTKIYHPNVDKLGRICLSTLKKDWSPALQIRTVLLSIQALMGAPNPDDPL 120
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 87.4 bits (207), Expect = 2e-18
Identities = 38/81 (46%), Positives = 53/81 (65%), Gaps = 1/81 (1%)
Frame = -1
Query: 405 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILRED-WKPVLT 229
P + Y GGKF FS K +YP +PP ++ T +YHPN D EGNVCL IL++ +KP +
Sbjct: 46 PSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIK 105
Query: 228 VNSIVYGLQYLFLEPNPEDPL 166
+ S++ + L EPNP+DPL
Sbjct: 106 LRSVLEQILQLLREPNPDDPL 126
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 86.6 bits (205), Expect = 3e-18
Identities = 44/116 (37%), Positives = 66/116 (56%), Gaps = 3/116 (2%)
Frame = -1
Query: 504 RITKDLNELNLPKTCN-TEFPDPD-DLLNFK-LIICPDEGFYRGGKFVFSFKVGPNYPHE 334
R+ +L L + T + FPD D +LL++ I P + +Y G KF S NYP+
Sbjct: 32 RLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYS 91
Query: 333 PPKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPL 166
PP + + ++HPN+D+ GN+CL+IL++ W V V +I+ LQ L EPN PL
Sbjct: 92 PPTIIFTSPMWHPNVDMSGNICLDILKDKWSAVYNVQTILLSLQSLLGEPNNASPL 147
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 82.2 bits (194), Expect = 6e-17
Identities = 41/115 (35%), Positives = 64/115 (55%), Gaps = 3/115 (2%)
Frame = -1
Query: 504 RITKDLNELNLPKTCNTE-FPDPDDLLNFKLIICPDEGF-YRGGKFVFSFKVGPNYPHEP 331
RI K+L ++ K + + DD+ + K + EG Y GG FV ++ +YP P
Sbjct: 8 RIAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFRP 67
Query: 330 PKVKCETAVYHPNIDLE-GNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDP 169
PK+ +T +YHPN+ + G +CL+IL++ W PV T+ S + LQ L P P +P
Sbjct: 68 PKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYTMKSALISLQSLLCTPEPSNP 122
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 66.9 bits (156), Expect = 3e-12
Identities = 33/92 (35%), Positives = 51/92 (55%), Gaps = 3/92 (3%)
Frame = -1
Query: 435 DLLNFKL-IICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNI 259
DL+N+K+ I + + GG + + YP PPK + ++HPN+ G VCL+I
Sbjct: 37 DLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSI 96
Query: 258 LRED--WKPVLTVNSIVYGLQYLFLEPNPEDP 169
L E+ WKP +T+ I+ G+Q L +PN P
Sbjct: 97 LNEEEGWKPAITIKQILLGIQDLLDDPNIASP 128
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 65.3 bits (152), Expect = 8e-12
Identities = 34/120 (28%), Positives = 61/120 (50%), Gaps = 2/120 (1%)
Frame = -1
Query: 522 ASAAQLRITKDLNELNL-PKTCNTEFPDPDDLLNFK-LIICPDEGFYRGGKFVFSFKVGP 349
++ A+ R+ +D + P + P D+++ + +II P + + G F
Sbjct: 2 STTARRRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDE 61
Query: 348 NYPHEPPKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDP 169
YP++PP VK + ++HPN+ G +CL+IL+ W P V +I+ +Q L +PN P
Sbjct: 62 QYPNKPPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNNASP 121
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 61.7 bits (143), Expect = 1e-10
Identities = 39/134 (29%), Positives = 65/134 (48%), Gaps = 16/134 (11%)
Frame = -1
Query: 522 ASAAQLRITKDLNEL--NLPKTCNTEFPDPDDLLNFK-LIICPDEGFYRGGKFVFSFKVG 352
+SA++ + K L E+ N P+ + D + ++ +II P++ Y GG F +
Sbjct: 3 SSASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFP 62
Query: 351 PNYPHEPPKVKCETAVYHPNIDLEGNVCLNILR-------------EDWKPVLTVNSIVY 211
+YP PPK+K T ++HPN+ G VC++IL E W PV + +I+
Sbjct: 63 QDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPETILI 122
Query: 210 GLQYLFLEPNPEDP 169
+ + PN E P
Sbjct: 123 SVISMLSSPNDESP 136
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 60.1 bits (139), Expect = 3e-10
Identities = 31/92 (33%), Positives = 46/92 (50%), Gaps = 2/92 (2%)
Frame = -1
Query: 435 DLLNFKLII-CPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNID-LEGNVCLN 262
D+ ++K +I P E Y GG++V V YP PP V +T + HPNI G VC++
Sbjct: 33 DMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMD 92
Query: 261 ILREDWKPVLTVNSIVYGLQYLFLEPNPEDPL 166
IL+ W P ++ S + L + PL
Sbjct: 93 ILKTHWSPAWSLQSACLAIISLLSNYDASSPL 124
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 56.8 bits (131), Expect = 3e-09
Identities = 28/82 (34%), Positives = 42/82 (51%), Gaps = 2/82 (2%)
Frame = -1
Query: 405 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNID-LEGNVCLNILREDWKPVL- 232
P E Y GG + ++ YP + P + ++HPNID L G+VCL+++ + W P+
Sbjct: 39 PSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFD 98
Query: 231 TVNSIVYGLQYLFLEPNPEDPL 166
+N L L PN DPL
Sbjct: 99 MINIFEVFLPQLLRYPNASDPL 120
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 56.8 bits (131), Expect = 3e-09
Identities = 41/133 (30%), Positives = 61/133 (45%), Gaps = 16/133 (12%)
Frame = -1
Query: 525 KASAAQLRITKDLNEL--NLPKTCNTEFPDPDDLLNFKLIIC-PDEGFYRGGKFVFSFKV 355
KA A + R+ K+ EL N P + DD + +I PD + GG + + K
Sbjct: 3 KAMALR-RLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKF 61
Query: 354 GPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILR-------------EDWKPVLTVNSIV 214
+YP PP +K E +HPN+ +G VC++IL E W PV +V I+
Sbjct: 62 PSDYPLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKIL 121
Query: 213 YGLQYLFLEPNPE 175
+ + EPN E
Sbjct: 122 LSVMSMLAEPNDE 134
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 33.5 bits (73), Expect = 0.029
Identities = 22/85 (25%), Positives = 40/85 (47%), Gaps = 4/85 (4%)
Frame = -1
Query: 447 PDPDDLLNFKLIIC-PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNV 271
P +++L + II P + Y GG++ + P+YP +PP ++ T +
Sbjct: 29 PATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRL 86
Query: 270 CLNIL---REDWKPVLTVNSIVYGL 205
CL+ + W P V++I+ GL
Sbjct: 87 CLSFSDFHPKSWNPSWMVSTILVGL 111
>SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex
subunit |Schizosaccharomyces pombe|chr 2|||Manual
Length = 509
Score = 29.5 bits (63), Expect = 0.48
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -2
Query: 596 LNFFH*NNRKKMKRVLPEEEDHKKKHQQP 510
+ +F NN K ++R+L E ED KH +P
Sbjct: 235 IRYFKHNNMKDLERILQELEDDFVKHNRP 263
>SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 543
Score = 25.4 bits (53), Expect = 7.8
Identities = 11/43 (25%), Positives = 22/43 (51%)
Frame = +2
Query: 410 IINLKFNRSSGSGNSVLHVFGRFNSFKSFVIRNWAADAFFCDP 538
II+L + +S + N+ ++FG F ++ + W+ F P
Sbjct: 214 IISLAASSTSETRNTGSYIFGNFENYSGWTNMGWSFILCFTTP 256
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,588,129
Number of Sequences: 5004
Number of extensions: 52953
Number of successful extensions: 204
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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