BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P17_pT_H08
(661 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF... 30 0.34
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 29 0.45
SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ... 29 0.59
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa... 29 0.78
SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9 |Sch... 27 3.2
SPAC29E6.02 |prp3|SPAC30.06|U4/U6 x U5 tri-snRNP complex subunit... 27 3.2
SPAC1B2.03c |||GNS1/SUR4 family protein|Schizosaccharomyces pomb... 25 9.7
>SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E
4F complex subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 243
Score = 29.9 bits (64), Expect = 0.34
Identities = 17/50 (34%), Positives = 24/50 (48%), Gaps = 2/50 (4%)
Frame = -2
Query: 660 IAIWTADAMKQHATIEIGKKLKEQLGIHGK--IGFQVHRDTMVKHSSATK 517
IA+WT + + +IG + KE LGI K I + H D+ S K
Sbjct: 189 IAVWTRNCNDKDVLEKIGLRFKEVLGISDKETIEYSAHEDSSKAGSMRAK 238
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 29.5 bits (63), Expect = 0.45
Identities = 21/83 (25%), Positives = 36/83 (43%), Gaps = 1/83 (1%)
Frame = +2
Query: 170 LPTSRVAYLTTYNYVTLHTDTRA*V*YKIRLLTFYSSRGLYHVTM*DKYDYKN*TNNTSV 349
+P ++ + Y Y+ L D +K + FY H M + +Y+N T S+
Sbjct: 1092 VPRNKTIFRILYEYIQLSDDHTLDDFWKTPVPIFYGEPDCIHNDMKGELNYENETEGFSI 1151
Query: 350 KYNFIL-LL*LRKFSLWDVLNIF 415
IL LL + + + DV +F
Sbjct: 1152 NIREILDLLSILYYGIRDVHTLF 1174
>SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F
complex subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 218
Score = 29.1 bits (62), Expect = 0.59
Identities = 15/55 (27%), Positives = 25/55 (45%), Gaps = 2/55 (3%)
Frame = -2
Query: 660 IAIWTADAMKQHATIEIGKKLKEQLGI--HGKIGFQVHRDTMVKHSSATKNLYTV 502
+A+WT + +EIG + K+ L + I F H D+ S+ K +V
Sbjct: 164 LAVWTKSCNNREVLMEIGTRFKQVLNLPRSETIEFSAHEDSSKSGSTRAKTRMSV 218
>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 783
Score = 28.7 bits (61), Expect = 0.78
Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
Frame = -1
Query: 583 DTRQDW-FPSTQRH-NG*A*FCDQESIHCLVLAL 488
D Q+W FP TQ++ N FC+Q H L+LAL
Sbjct: 98 DAMQNWIFPQTQQYRNYQKEFCEQALFHNLLLAL 131
>SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 577
Score = 26.6 bits (56), Expect = 3.2
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = -2
Query: 381 RNYNNSIKLYLTEVLFV 331
RN+N ++ L+LT VLFV
Sbjct: 118 RNFNRAVALHLTSVLFV 134
>SPAC29E6.02 |prp3|SPAC30.06|U4/U6 x U5 tri-snRNP complex subunit
Prp3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 542
Score = 26.6 bits (56), Expect = 3.2
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +2
Query: 116 INMSVKHIIPVSSR*FHNLPTSRVAYLTTYNYVTLHTDTRA 238
IN +++H IPV N P+S +LT + TRA
Sbjct: 271 INSAIQHPIPVLPPYAKNQPSSHSVFLTKKEQKKIRRQTRA 311
>SPAC1B2.03c |||GNS1/SUR4 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 334
Score = 25.0 bits (52), Expect = 9.7
Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 4/48 (8%)
Frame = -3
Query: 581 YTARLVSKYTE---TQWLSIVLRPRIYT-LFSFGITYLVCICRFMGRT 450
Y ++KY E T +L + +P + + GIT L+C + +GRT
Sbjct: 137 YYLNYLTKYLELMDTVFLFLKKKPLAFLHCYHHGITALLCFTQLLGRT 184
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,365,094
Number of Sequences: 5004
Number of extensions: 43557
Number of successful extensions: 73
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 73
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 73
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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