BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P17_F_O21
(731 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 120 3e-28
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 33 0.042
SPBC776.05 |||membrane transporter |Schizosaccharomyces pombe|ch... 28 1.2
SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces pomb... 27 2.1
SPAC824.08 |gda1|gdp1|guanosine-diphosphatase Gda1|Schizosacchar... 27 2.8
SPCC1620.11 |mug87||nucleoporin Nic96 homolog|Schizosaccharomyce... 27 3.6
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 6.4
SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 26 6.4
>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 538
Score = 120 bits (288), Expect = 3e-28
Identities = 58/111 (52%), Positives = 81/111 (72%), Gaps = 5/111 (4%)
Frame = +2
Query: 413 SSCRKVKEEVSKLLALKAQLV--TEDAGPQK---FTLKTPKGTRDYNPQQMTIRNNVLDK 577
+S + +EV+KLL LK + +E +G +K FTLKTPKGT+D+ + + +R ++
Sbjct: 27 ASKEDIDKEVAKLLQLKNLKLGGSEVSGKKKDTSFTLKTPKGTKDWCDKDVVLREHIFST 86
Query: 578 IITVFRRHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLXYD 730
+ +F+RHG +DTPVFELK++L+GKYGEDSKLIYDLKDQGGE+ SL YD
Sbjct: 87 VTEIFKRHGGVTLDTPVFELKEILSGKYGEDSKLIYDLKDQGGELCSLRYD 137
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 33.1 bits (72), Expect = 0.042
Identities = 19/55 (34%), Positives = 31/55 (56%)
Frame = +2
Query: 566 VLDKIITVFRRHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLXYD 730
V D ++ VFRRHGA+ ++ + K + +Y +D + L D+ G +L L YD
Sbjct: 990 VRDHVVKVFRRHGAKERESHILFPK---SSQYDKDQASV-SLLDKNGTLLQLPYD 1040
>SPBC776.05 |||membrane transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 404
Score = 28.3 bits (60), Expect = 1.2
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = -2
Query: 595 PKNCNNFIQYIITYCHLLWVVVSCSLWCFKSKL 497
P+N F+ II Y + + ++ CSLW + +KL
Sbjct: 318 PQNLQPFVFMIIQYLYSITTMLPCSLW-YNNKL 349
>SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1136
Score = 27.5 bits (58), Expect = 2.1
Identities = 16/60 (26%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Frame = +2
Query: 554 IRNNVLDKIITVFR-RHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLXYD 730
++N VL I++ + ++ +E K +L ++ +KL+ L G EILS+ +D
Sbjct: 466 LKNAVLSSIVSTIQIEPNSDLTGYDYYEYKRLLYNEWERFAKLVAYLDHFGDEILSINFD 525
>SPAC824.08 |gda1|gdp1|guanosine-diphosphatase
Gda1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 556
Score = 27.1 bits (57), Expect = 2.8
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -3
Query: 675 FESSPYFPVSTSFSSKTGVS 616
F P FPV+TS +KT VS
Sbjct: 78 FSEEPLFPVTTSIPTKTAVS 97
>SPCC1620.11 |mug87||nucleoporin Nic96 homolog|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 851
Score = 26.6 bits (56), Expect = 3.6
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +1
Query: 64 CPAICSLTPALARFXLVKSRXF 129
CP+ICS+T F L+ SR F
Sbjct: 418 CPSICSVTEDYIWFQLILSREF 439
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 25.8 bits (54), Expect = 6.4
Identities = 17/55 (30%), Positives = 31/55 (56%), Gaps = 2/55 (3%)
Frame = +2
Query: 524 TRDYNPQQMTIRNNVLDKIITVFRRHGAECIDTPVFELKDV--LTGKYGEDSKLI 682
TRDY+ +Q+ R NV+ +++ F + A+ ++ P +K + L G Y E L+
Sbjct: 2511 TRDYHIKQVNNRPNVISTLVSSFVKCAAK-LELPPHLVKYLGKLYGVYHESVSLL 2564
>SPAPB8E5.07c |||ribosome biogenesis protein
Rrp12|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1163
Score = 25.8 bits (54), Expect = 6.4
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -3
Query: 276 IFITRPFLVPLIGILRCF*FSNK 208
+FI+ PFLV LI L F S+K
Sbjct: 857 VFISEPFLVQLISTLNLFITSSK 879
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,908,645
Number of Sequences: 5004
Number of extensions: 58394
Number of successful extensions: 143
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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