Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P17_F_J07
(674 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 47 3e-06
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 45 1e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.007
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 32 0.087
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 31 0.15
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 29 0.46
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 29 0.61
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 28 1.1
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 1.9
SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 27 2.5
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 27 3.3
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.3
SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|c... 26 4.3
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 26 4.3
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 5.7
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 25 7.6
SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces pomb... 25 7.6
SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr 2... 25 7.6
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 25 10.0
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 25 10.0
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 10.0
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 46.8 bits (106), Expect = 3e-06
Identities = 25/89 (28%), Positives = 46/89 (51%), Gaps = 1/89 (1%)
Frame = +3
Query: 363 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSES 542
D DG+ + E ++ G +E +++ ++ D D +G+I EFL + M ++
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDT 81
Query: 543 -RRNIVEQAFKKLDKTGDGAITIDDIKGV 626
V +AFK DK G+G IT++++ V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHV 110
Score = 37.1 bits (82), Expect = 0.002
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +3
Query: 351 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEF 509
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 44.8 bits (101), Expect = 1e-05
Identities = 33/144 (22%), Positives = 67/144 (46%), Gaps = 6/144 (4%)
Frame = +3
Query: 234 EELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGI 413
E + ++ + + +++ L + AS L R+F +D+DG ++ +EF+ +
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDEDGGGDVDFQEFINSL 82
Query: 414 KETGLELNKSEAEEL-FSQFDTDNSGSISLDEFLIKIRPPMSESRR-----NIVEQAFKK 575
+ NK E + F +D D G IS E + ++ + + R IV++ +
Sbjct: 83 SVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIME 142
Query: 576 LDKTGDGAITIDDIKGVYSVDSQT 647
+DK DG I+ ++ K + S + T
Sbjct: 143 VDKDRDGKISFEEFKDIVSGSNVT 166
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.5 bits (78), Expect = 0.007
Identities = 18/97 (18%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
Frame = +3
Query: 339 LGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFL-I 515
+ F+ D D ++ E ++ G KSE ++ FD G + +++F+ +
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 516 KIRPPMSESRRNIVEQAFKKLDKTGDGAITIDDIKGV 626
+ +++AF+ D G I++ +++ V
Sbjct: 99 MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRV 135
Score = 33.1 bits (72), Expect = 0.038
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +3
Query: 345 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFL 512
R F DDD + ++ KE ++ E E + +FD D G I+ EF+
Sbjct: 114 RAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
Score = 32.7 bits (71), Expect = 0.050
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = +3
Query: 429 ELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 608
E + + E F FD+D +I E +R + ++ V + + DKTG G + +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 609 DD 614
+D
Sbjct: 93 ED 94
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 31.9 bits (69), Expect = 0.087
Identities = 22/96 (22%), Positives = 40/96 (41%), Gaps = 2/96 (2%)
Frame = +3
Query: 336 GLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLI 515
G+ ++FR + S +LN+ EF + GL + E LF + G ++ + F
Sbjct: 488 GITKVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG-VTYERFTE 546
Query: 516 KIRPPMSESRRNIVEQAFKKLDKTGDGA--ITIDDI 617
+ + + R+ Q DG +T DD+
Sbjct: 547 IVMEELED--RDSARQVLYAFCDVADGKSYVTSDDL 580
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 31.1 bits (67), Expect = 0.15
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 1/79 (1%)
Frame = +3
Query: 384 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVE 560
LNK EF K+ + S AE +F+ FD D +G I EF+ + + +
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLI 102
Query: 561 QAFKKLDKTGDGAITIDDI 617
AF+ D +G I+ D++
Sbjct: 103 WAFQLYDLDNNGLISYDEM 121
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 29.5 bits (63), Expect = 0.46
Identities = 18/74 (24%), Positives = 31/74 (41%)
Frame = +3
Query: 396 EFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKK 575
E+ G+ + + S LF +FD +GS+SL + + I + F+
Sbjct: 564 EWAKGLDAAAINNSSSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISFIFEL 623
Query: 576 LDKTGDGAITIDDI 617
D GDG + D+
Sbjct: 624 YDFNGDGFMDKPDV 637
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 29.1 bits (62), Expect = 0.61
Identities = 23/80 (28%), Positives = 40/80 (50%), Gaps = 2/80 (2%)
Frame = +3
Query: 426 LELNKSEA--EELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGA 599
L LN S EE F + D D+SG +S +EF + + ++R IV+ FK+ +G
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEFQHFV--SLLKTRSEIVD-IFKEY-TSGSDK 381
Query: 600 ITIDDIKGVYSVDSQTRYKS 659
++++ + S + R S
Sbjct: 382 MSLEQFRHFLSTSQKARLDS 401
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 28.3 bits (60), Expect = 1.1
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +1
Query: 439 KVKPKNSSVNSTQTIVAQSVLMNSLLKSVLLCRNRVVT-L*NKHSRSLTRLVTVQSQL 609
K+ KN+ S + + ++ L L L + +++ L NK S TRL +QSQL
Sbjct: 765 KLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQL 822
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 27.5 bits (58), Expect = 1.9
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +3
Query: 531 MSESRRNIVEQAFKKLDKTGDGAITIDDIK 620
++ S+ +++AF LDK GDG I +D+K
Sbjct: 42 LTSSQIQELKEAFALLDKDGDGNIGREDVK 71
>SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 488
Score = 27.1 bits (57), Expect = 2.5
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +3
Query: 171 KPKTSSNIAWHRPMSAGSVQEEELMQK 251
KPK S ++W+ + GS +E EL+ K
Sbjct: 56 KPKKDSLLSWNILLKKGSYKENELLAK 82
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 26.6 bits (56), Expect = 3.3
Identities = 10/34 (29%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -3
Query: 408 HKGIL-LCSVVYFHHHPCDGKFFQVPRSLKHLET 310
H+G++ +C++VY KF + P++++ L T
Sbjct: 686 HRGLVCICNIVYSKDQEIFNKFIKTPKAVETLRT 719
>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 26.2 bits (55), Expect = 4.3
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = +3
Query: 594 GAITIDDIKGVYSVDSQTRYKSGEXTE 674
G I+I D GVYS + T YKS E E
Sbjct: 126 GVISIHDSTGVYSQITTTPYKSLEDYE 152
>SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 521
Score = 26.2 bits (55), Expect = 4.3
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +2
Query: 5 RPMSLSSHPSLMRRLG 52
RP +S+HP ++RRLG
Sbjct: 348 RPQVISNHPEIIRRLG 363
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 26.2 bits (55), Expect = 4.3
Identities = 21/71 (29%), Positives = 32/71 (45%)
Frame = +3
Query: 399 FLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKKL 578
F+ +E+ LE E FSQ ++ + SL+ I P SRR IVEQ ++
Sbjct: 680 FILPNEESLLEKYWINYNESFSQLSRESLFT-SLESPFTDIESPTIVSRRKIVEQRKLRM 738
Query: 579 DKTGDGAITID 611
+K +D
Sbjct: 739 EKESFQETNVD 749
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 5.7
Identities = 10/34 (29%), Positives = 17/34 (50%)
Frame = -1
Query: 602 DCTVTSLVKLLECLFYNVTTRFRHRRTDFNKEFI 501
D TV + +E LF+N+T +H F + +
Sbjct: 699 DATVANRKDFIEFLFHNITVSSKHTAVIFTSDLL 732
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 25.4 bits (53), Expect = 7.6
Identities = 10/31 (32%), Positives = 16/31 (51%)
Frame = +1
Query: 292 NCACFACLEVLQGSWDLEEFSVAWMMMEVNY 384
N F +E + +D EEFSV W + + +
Sbjct: 41 NTTAFWFVEFTESKYDKEEFSVIWNEVSMEF 71
>SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 840
Score = 25.4 bits (53), Expect = 7.6
Identities = 19/60 (31%), Positives = 27/60 (45%), Gaps = 5/60 (8%)
Frame = +3
Query: 444 EAEELFSQFDTDNSGSISLDEF-----LIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 608
E ++F D D S +++LDE I I S V+ A KLD+ G G + I
Sbjct: 396 EINDIFHILDNDYSRTVTLDEMEQFTREISIEFRSISSSLRDVDLALGKLDRVGLGVVGI 455
>SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 497
Score = 25.4 bits (53), Expect = 7.6
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 5/101 (4%)
Frame = +3
Query: 222 SVQEEELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEF 401
S Q E + R M L +L + ++ ILGL R D+G K+ NK+
Sbjct: 182 SYQNLESINSWLRLMASLYKDLSELVPVGITAEGRTILGLKLNGRHPSDNGEKIRNKKVI 241
Query: 402 L-----YGIKETGLELNKSEAEELFSQFDTDNSGSISLDEF 509
+ + + G+ A +L +++D+D LD+F
Sbjct: 242 IIQGGSHAREWIGIPSVCYAAWQLLAKYDSDGHVRKLLDKF 282
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 25.0 bits (52), Expect = 10.0
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +3
Query: 348 IFRRMDDDGSKLLNKEEFLYGIKETGL-ELNKSEAE 452
IFRR+ D S + ++ FL+ G+ E+ +EAE
Sbjct: 377 IFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAE 412
>SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1369
Score = 25.0 bits (52), Expect = 10.0
Identities = 25/100 (25%), Positives = 43/100 (43%), Gaps = 1/100 (1%)
Frame = +3
Query: 279 DPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEEL 458
D E+ + S +S + L I + GS+L E F I G+ + AE
Sbjct: 793 DNYEQAESIISSLSSSALSELSYISESSMNIGSRL--DERF---IDANGVAIRDFSAE-- 845
Query: 459 FSQFDTDNS-GSISLDEFLIKIRPPMSESRRNIVEQAFKK 575
+ +NS G +S+D FL K++ + + + F+K
Sbjct: 846 LTYLTPENSKGKLSIDHFLNKVQSRWHDEEHHYYKTGFRK 885
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 25.0 bits (52), Expect = 10.0
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = +3
Query: 453 ELFSQFDTDNSGSISLDE 506
+LF QFD DN G+++ +E
Sbjct: 311 DLFYQFDRDNDGALNNEE 328
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,423,575
Number of Sequences: 5004
Number of extensions: 44882
Number of successful extensions: 175
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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