Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P17_F_G05
(772 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|... 62 1e-10
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma... 34 0.026
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo... 29 0.56
SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c... 29 0.97
SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 28 1.3
SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|ch... 28 1.3
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 27 3.0
SPAC26F1.08c |||conserved protein|Schizosaccharomyces pombe|chr ... 27 3.9
SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom... 26 6.9
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr... 25 9.1
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 9.1
>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 137
Score = 61.7 bits (143), Expect = 1e-10
Identities = 27/57 (47%), Positives = 38/57 (66%)
Frame = +3
Query: 303 KQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVEEKL 473
+ K+ +SW PD A +K KM+YSSS D L+++ G+ IQATD SE + E V EK+
Sbjct: 78 RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIGTDIQATDFSEVAYETVLEKV 134
Score = 52.4 bits (120), Expect = 7e-08
Identities = 27/71 (38%), Positives = 40/71 (56%)
Frame = +2
Query: 53 SGVTVSDACKXTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 232
SGV VS C ++E+K K RYVVF + D K V + +++ FL DL +
Sbjct: 4 SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPE---K 60
Query: 233 ECRYGLFDFEY 265
+CRY ++DFE+
Sbjct: 61 DCRYAIYDFEF 71
>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 328
Score = 33.9 bits (74), Expect = 0.026
Identities = 15/27 (55%), Positives = 21/27 (77%)
Frame = +3
Query: 297 SKKQKLFLMSWCPDTAKVKKKMLYSSS 377
SKK L L+S+ P+ A V++KMLY+SS
Sbjct: 76 SKKNLLQLISYVPENANVRRKMLYASS 102
Score = 28.7 bits (61), Expect = 0.97
Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 5/56 (8%)
Frame = +3
Query: 309 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 461
K+ + CP A VK +M+YSSS D++K L + + I++ D ++ +++ +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299
>SPAC17H9.11 |||cofilin/tropomyosin family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 141
Score = 29.5 bits (63), Expect = 0.56
Identities = 17/63 (26%), Positives = 30/63 (47%)
Frame = +3
Query: 285 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 464
T++ LF++ W P M+Y+S+ + + V K +A D + + EAV+
Sbjct: 79 TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137
Query: 465 EKL 473
E L
Sbjct: 138 EFL 140
>SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor
complex subunit Rrn5|Schizosaccharomyces pombe|chr
1|||Manual
Length = 556
Score = 28.7 bits (61), Expect = 0.97
Identities = 23/95 (24%), Positives = 42/95 (44%), Gaps = 4/95 (4%)
Frame = -1
Query: 544 VSESGFGFVASSCVNAVYWRSVARSFSSTAS*DASERSVAWMYF*TPTR---DFFRASNE 374
+ E +G ++ SCV+ YW + + A +R + + + P++ R N
Sbjct: 56 LEEEQWGVLSGSCVDGTYWSAEEKELFFQAVARNGKRDLDLIAYSIPSKSAVQIERYINA 115
Query: 373 LEYNIFFLTLAVSGHHDIR-KSFCFLLASDVPLAL 272
LE + +L H D +S C L D+P+A+
Sbjct: 116 LENELRWL----RNHVDASVRSQCLLKYEDIPIAM 146
>SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 314
Score = 28.3 bits (60), Expect = 1.3
Identities = 24/91 (26%), Positives = 34/91 (37%)
Frame = +3
Query: 267 RTSARGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEA 446
RT T A+K + P T K + ++ A KK + G +KY + S
Sbjct: 124 RTQFAFTGRATKSNAGVMDVQSPSTMSTSKNNVRNAERPASKKPVFGSKKYFDVINDSNV 183
Query: 447 SQEAVEEKLRATDRQ*TAFTHELATKPNPLS 539
+ E K R DR + FT LS
Sbjct: 184 ENKE-ETKDRNLDRALSKFTQSREKNEQNLS 213
>SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 28.3 bits (60), Expect = 1.3
Identities = 16/53 (30%), Positives = 23/53 (43%), Gaps = 2/53 (3%)
Frame = -3
Query: 392 FQSVERARVQHLLLDLGGVRAPRHQEELLFLASLRRAPGT--GACIQSQTGHI 240
+Q + RA + H + + Q E +F+ LRR PGT G C I
Sbjct: 113 YQVLTRACITHNIRHICTAHHANDQAETIFMRLLRRKPGTWGGLCAMKPVSQI 165
>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 214
Score = 27.1 bits (57), Expect = 3.0
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = +3
Query: 375 SFDALKKSLVGVQKYIQATDLSEASQ 452
S +AL++ L G + Y+Q+TD++ S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213
>SPAC26F1.08c |||conserved protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 977
Score = 26.6 bits (56), Expect = 3.9
Identities = 16/32 (50%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
Frame = -3
Query: 395 LFQSVERAR-VQHLLLDLGGVRAPRHQEELLF 303
LF E AR V++LLL L G++A RH + LF
Sbjct: 460 LFNLFEVAREVKNLLLLLEGLKAQRHNKPKLF 491
>SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 447
Score = 25.8 bits (54), Expect = 6.9
Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 3/60 (5%)
Frame = +2
Query: 437 LGSVSGGRRREAPRHRSPINSIYTRARDE---TEPALRHSCPDDTRPRHH*PLSIITKEN 607
LG G ++ E+ R + P S + EP +RHS RP+ H S +T+++
Sbjct: 20 LGEEDGRQKGESSRKKRPPLSRKNPSNVSFWSNEPIIRHSSVKTDRPQFHRADSTVTEQS 79
>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 319
Score = 25.4 bits (53), Expect = 9.1
Identities = 14/61 (22%), Positives = 31/61 (50%)
Frame = +2
Query: 80 KXTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 259
K + + ++ + K ++ +D K+ D + + + EY F ++L+ TG+ Y DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166
Query: 260 E 262
+
Sbjct: 167 Q 167
>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 357
Score = 25.4 bits (53), Expect = 9.1
Identities = 13/46 (28%), Positives = 20/46 (43%), Gaps = 2/46 (4%)
Frame = -2
Query: 279 WHWCVYSKSNRPY--LHSPVPPFCRSSRNCSYSALRSPTVSTSICF 148
W++ Y+ S R Y VPP+CR +R+ + CF
Sbjct: 122 WNFVDYALSLRKYGWTDDVVPPYCRKVLTYKSLGIRTAVYVGAFCF 167
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,887,845
Number of Sequences: 5004
Number of extensions: 55511
Number of successful extensions: 183
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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