Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P17_F_D24
(708 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p... 36 0.004
SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 35 0.013
SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity... 34 0.023
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 32 0.070
SPCC1827.05c |||nucleolar RNA-binding protein NIFK |Schizosaccha... 31 0.21
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 31 0.21
SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual 29 0.49
SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccha... 29 0.65
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 29 0.86
SPBC14C8.04 |||acetolactate synthase regulatory unit|Schizosacch... 28 1.5
SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyc... 27 2.0
SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po... 27 2.0
SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces po... 27 2.6
SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 27 3.5
SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster ... 27 3.5
SPBC13A2.01c |||nuclear cap-binding complex small subunit|Schizo... 26 4.6
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 26 6.1
SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharo... 26 6.1
>SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 517
Score = 36.3 bits (80), Expect = 0.004
Identities = 36/149 (24%), Positives = 63/149 (42%), Gaps = 16/149 (10%)
Frame = +1
Query: 283 WDVPPPGFEHITPLQYK---------AMQAAGQIPANIV--ADTPQAAV----PVVGSTI 417
WD+ PPG+E +T Q K A +AA P ++ A + + ++ P +
Sbjct: 135 WDIKPPGYELVTADQAKMSGVFPLPGAPRAAVTDPEKLLEFARSAEGSIIAPPPPLQPGA 194
Query: 418 TRQARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRS 597
+RQARRL V IP E+ + F + + + + ++NFA LE +
Sbjct: 195 SRQARRLVVTGIPNEFVEDAFVSFIEDLFISTTYHKPETKHFSSVNVCKEENFAILEVAT 254
Query: 598 IDETTQAMAFDGINFKGQ-SLKIRRPHDY 681
++ T ++ LK +R +Y
Sbjct: 255 PEDATFLWGLQSESYSNDVFLKFQRIQNY 283
>SPAC328.05 |||RNA-binding protein involved in export of
mRNAs|Schizosaccharomyces pombe|chr 1|||Manual
Length = 434
Score = 34.7 bits (76), Expect = 0.013
Identities = 25/92 (27%), Positives = 39/92 (42%), Gaps = 1/92 (1%)
Frame = +1
Query: 406 GSTITRQARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFL 585
G T+Q RR+YVGN+ + V E EF Q + +L L K A +
Sbjct: 58 GQKYTQQERRVYVGNLSYQVRWFELKEFMGQ------VGNVLNCEILNLPNGLSKGCAII 111
Query: 586 EFRSIDETTQAM-AFDGINFKGQSLKIRRPHD 678
E+ + +E A+ F G+ + IR +
Sbjct: 112 EYSTAEEARTAIKTLSNQKFMGRLVYIREDRE 143
Score = 28.7 bits (61), Expect = 0.86
Identities = 18/87 (20%), Positives = 40/87 (45%), Gaps = 1/87 (1%)
Frame = +1
Query: 436 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQ 615
+YVGN+P+ ++ ++ F + A+ A P K F ++F + ++
Sbjct: 282 IYVGNLPWATSDRNLLDLFTDIGSVI-RARIAYEPT-----GRSKGFGVVQFENENDAAS 335
Query: 616 AM-AFDGINFKGQSLKIRRPHDYXPMP 693
++ +G + G+ L++ H P+P
Sbjct: 336 SIEKLNGYRYGGRPLQLSYAHYATPLP 362
>SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ctf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 363
Score = 33.9 bits (74), Expect = 0.023
Identities = 23/68 (33%), Positives = 33/68 (48%)
Frame = +1
Query: 436 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQ 615
++VGNIP+ V+E++ E FNQ + VL + K + F EF D T
Sbjct: 9 VFVGNIPYDVSEQQMTEIFNQVGPVKTF-----KLVLDPETGSGKGYGFCEF--FDSETT 61
Query: 616 AMAFDGIN 639
AMA +N
Sbjct: 62 AMAVRKLN 69
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 32.3 bits (70), Expect = 0.070
Identities = 19/75 (25%), Positives = 37/75 (49%)
Frame = +1
Query: 439 YVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQA 618
+VGN+ F +TE + +FF + + L V+ + F ++EF + D + A
Sbjct: 97 HVGNLSFDLTENDLGDFFGEGVTSIRL-------VIDPLTERSRGFGYVEFETADTLSAA 149
Query: 619 MAFDGINFKGQSLKI 663
+A G + G+ ++I
Sbjct: 150 LALSGEDLMGRPVRI 164
>SPCC1827.05c |||nucleolar RNA-binding protein NIFK
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 276
Score = 30.7 bits (66), Expect = 0.21
Identities = 17/56 (30%), Positives = 29/56 (51%)
Frame = +1
Query: 436 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSID 603
LYVG +P G E++ +F+Q + L + + K++AF+EF S+D
Sbjct: 107 LYVGRLPHGFYEKQMRMYFSQFGPVLRLRMSRNR-----KTGSSKHYAFIEFESLD 157
>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 422
Score = 30.7 bits (66), Expect = 0.21
Identities = 21/68 (30%), Positives = 34/68 (50%)
Frame = +1
Query: 436 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQ 615
+YVGNIP+ + EE+ ++ F Q SG ++ V+ + K + F E+ D T
Sbjct: 7 VYVGNIPYEMAEEQVIDIFKQ----SGPVKSF-QLVIDPESGQPKGYGFCEYH--DPATA 59
Query: 616 AMAFDGIN 639
A A +N
Sbjct: 60 ASAVRNLN 67
>SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 29.5 bits (63), Expect = 0.49
Identities = 21/80 (26%), Positives = 34/80 (42%), Gaps = 1/80 (1%)
Frame = +1
Query: 427 ARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQI-NLDKNFAFLEFRSID 603
A+ +YVGN+ + VT EE Q H + +L + K FA++EF
Sbjct: 54 AQSVYVGNVDYSVTPEEL------QSHFASCGSVNRVTILCDKFTGHPKGFAYIEFSEPS 107
Query: 604 ETTQAMAFDGINFKGQSLKI 663
A+ +G + LK+
Sbjct: 108 LVPNALLLNGSMLHERPLKV 127
>SPBC660.15 |||mRNA cleavage factor complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 474
Score = 29.1 bits (62), Expect = 0.65
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +1
Query: 433 RLYVGNIPFGVTEEETMEFFNQ 498
+++VG +P TEEE FFNQ
Sbjct: 248 KMFVGGVPGDCTEEEFRNFFNQ 269
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 28.7 bits (61), Expect = 0.86
Identities = 25/91 (27%), Positives = 47/91 (51%), Gaps = 1/91 (1%)
Frame = +1
Query: 352 QIPANIVADTPQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFF-NQQMHLSGLAQA 528
Q P++ + T + + P V + R L++GNIP +E +F+ ++ + LSG +
Sbjct: 113 QTPSSEKSTTERESRPFVPPNSQQMRRMLFIGNIP-----KELDDFWMDKILRLSG--KL 165
Query: 529 AGNPVLACQINLDKNFAFLEFRSIDETTQAM 621
A +A N +F F EF S ++ ++A+
Sbjct: 166 ASWRRVADADNSMTSFGFAEFESNEQFSRAL 196
>SPBC14C8.04 |||acetolactate synthase regulatory
unit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 289
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +1
Query: 316 TPLQYKAMQAAGQIPANIVADTPQAAVPVVGSTI 417
+P+ YKA+ A +P + + P+A VP S I
Sbjct: 23 SPITYKALHANSPLPRCRIIEPPRATVPEAVSNI 56
>SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 529
Score = 27.5 bits (58), Expect = 2.0
Identities = 21/78 (26%), Positives = 38/78 (48%)
Frame = +1
Query: 430 RRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDET 609
R +++GN+P G+TE+E E + G P+ +I ++N AF+ F +I
Sbjct: 206 RNVFLGNLPNGITEDEIRE----DLEPFG-------PIDQIKIVTERNIAFVHFLNIAAA 254
Query: 610 TQAMAFDGINFKGQSLKI 663
+A+ +N K +I
Sbjct: 255 IKAVQELPLNPKWSKRRI 272
>SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1014
Score = 27.5 bits (58), Expect = 2.0
Identities = 15/45 (33%), Positives = 20/45 (44%)
Frame = +1
Query: 358 PANIVADTPQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFF 492
P + P P G+ + R LYV NI F V E++ FF
Sbjct: 735 PIQVFISDPLRRTPRSGAVY--EGRELYVTNIDFKVNEKDVETFF 777
>SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 365
Score = 27.1 bits (57), Expect = 2.6
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +1
Query: 433 RLYVGNIPFGVTEEETMEFF 492
RL+VG IP T E+ M+FF
Sbjct: 5 RLFVGRIPPQATREDMMDFF 24
>SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 561
Score = 26.6 bits (56), Expect = 3.5
Identities = 17/59 (28%), Positives = 26/59 (44%)
Frame = +1
Query: 328 YKAMQAAGQIPANIVADTPQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFFNQQM 504
+ A ++ I I A TP P + +YVGN+P +EEE F+ Q+
Sbjct: 395 FSAYSSSHGIHQRIPASTPTNTNPADQNP---PCNTIYVGNLPPSTSEEELKVLFSTQV 450
>SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster
type |Schizosaccharomyces pombe|chr 1|||Manual
Length = 782
Score = 26.6 bits (56), Expect = 3.5
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = +2
Query: 392 PCRWWVRL*HVKREGYMSATYPLASP 469
P RWWV H G + A Y L P
Sbjct: 550 PLRWWVFRFHSLNAGIIQAAYALCFP 575
>SPBC13A2.01c |||nuclear cap-binding complex small
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 182
Score = 26.2 bits (55), Expect = 4.6
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +1
Query: 421 RQARRLYVGNIPFGVTEEETMEFFNQ 498
+Q+ +YVGN+ F TEE+ F++
Sbjct: 29 KQSNCVYVGNLSFYTTEEQIYALFSK 54
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 25.8 bits (54), Expect = 6.1
Identities = 19/86 (22%), Positives = 36/86 (41%)
Frame = +1
Query: 406 GSTITRQARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFL 585
G ++ + ++VGN+ F TE++ F G Q+ P Q K F ++
Sbjct: 358 GDQLSEPSDTVFVGNLSFNATEDDLSTAFGG----CGDIQSIRLPT-DPQSGRLKGFGYV 412
Query: 586 EFRSIDETTQAMAFDGINFKGQSLKI 663
F ID + + +G G+ ++
Sbjct: 413 TFSDIDSAKKCVEMNGHFIAGRPCRL 438
>SPAC1687.20c |mis6||inner centromere protein
Mis6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 672
Score = 25.8 bits (54), Expect = 6.1
Identities = 15/44 (34%), Positives = 19/44 (43%)
Frame = +1
Query: 382 PQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFFNQQMHLS 513
PQ + G I Q G+IP +EET F +HLS
Sbjct: 597 PQFFSYINGYQIYLQQTNPATGSIPLKPIQEETFGAFQSNLHLS 640
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,699,457
Number of Sequences: 5004
Number of extensions: 55892
Number of successful extensions: 161
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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